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EC number: 817-187-7 | CAS number: 1803088-15-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-09-16 to 2016-09-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1997
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Version / remarks:
- 1998
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 5,7-Di-t-butyl-3-[3,5-dimethyl-4-[(1,3,7,9-tetra-t-butyl-5-methyl-5H-benzo[d][1,3,2]benzodioxaphosphocin-11-yl)oxy]phenyl]-3H-benzofuran-2-one
- EC Number:
- 817-187-7
- Cas Number:
- 1803088-15-4
- Molecular formula:
- C54 H73 O5 P
- IUPAC Name:
- 5,7-Di-t-butyl-3-[3,5-dimethyl-4-[(1,3,7,9-tetra-t-butyl-5-methyl-5H-benzo[d][1,3,2]benzodioxaphosphocin-11-yl)oxy]phenyl]-3H-benzofuran-2-one
- Details on test material:
- - State of aggregation: Solid / white
- Storage Condition: room temperature under light exclusion
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature; under light exclusion ; no direct sunlight
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- induced rat liver S9 mix
- Test concentrations with justification for top dose:
- 1st experiment and 2nd experiment:
0; 33; 100; 333; 1000; 2500; 5000 µg/plate
Top dose of 5 mg/plate was selected in agreement with the current guidelines. - Vehicle / solvent:
- - Vehicle/solvent used: DMSO
- Justification for choice of solvent/vehicle: DMSO has been demonstrated to be suitable for bacterial reverse mutation tests and historical control data are available.
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- other: 4-nitro-o-phenylenediamine (NOPD) without S9, strain: TA 98; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) without S9, strains: TA 1535 and TA 1537; 2-aminoanthracene (2-AA) with S9 all strains and without S9, strains: TA1537
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation) and preincubation
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 - 72 h
SELECTION AGENT: -his and -trp deficient medium
NUMBER OF REPLICATIONS: 3 plates per dose
DETERMINATION OF CYTOTOXICITY
- decrease in the number of revertants (factor <= 0.6)
- clearing or diminution of the background lawn - Evaluation criteria:
- The test substance was considered positive in this assay if the following criteria were met:
- A dose-related and reproducible increase in the number of revertant colonies, i.e. at least doubling (bacteria strains with high spontaneous mutation rate, like TA 98, TA 100 and E.coli WP2 uvrA) or tripling (bacteria strains with low spontaneous mutation rate, like TA 1535 and TA 1537) of the spontaneous mutation rate in at least one tester strain either without S9 mix or after adding a metabolizing system.
A test substance was generally considered non-mutagenic in this test if:
- The number of revertants for all tester strains were within the range of the historical negative control data under all experimental conditions in at least two experiments carried out independently of each other.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: none
- Effects of osmolality: none
- Evaporation from medium: no
- Water solubility: DMSO was used as vehicle
- Precipitation: was found from 33 µg/plate onward with and without S9 mix
HISTORICAL CONTROL DATA
- Positive historical control data: please refer to "Any other information on results" table 4
- Negative (solvent/vehicle) historical control data: please refer to "Any other information on results" table 4
CYTOTOXICITY
A weak bacteriotoxic effect (slight decrease in the number of his+ or trp+ revertants) was occasionally observed in the standard plate test depending on the strain and test conditions from about 1000 μg/plate onward. In the preincubation assay weak bacteriotoxicity (slight decrease in the number of his+ revertants) was occasionally observed depending on the strain and test conditions from about 33 μg/plate onward. Please also refer to "Any other information on results" table 3.
Any other information on results incl. tables
Table 1. Experiment 1 standard plate incorporation test
Dose (µg/plate) |
Mean number of revertant colonies of 3 replicates (± S.D.) for different strains of S. typhimurium and E. coli |
||||
|
TA 100 |
TA 98 |
TA 1535 |
TA 1537 |
WP2 uvrA |
|
|
||||
|
Results without S9 |
||||
DMSO |
103.0 ± 14.0 |
21.7 ± 4.2 |
8.3 ± 1.5 |
8.0 ± 1.0 |
19.7 ± 1.2 |
33 |
107.0 ± 7.0 |
28.3 ± 6.4 |
12.7 ± 2.1 |
6.0 ± 1.0 |
13.0 ± 4.4 |
100 |
107.3 ± 15.0 |
25.0 ± 9.5 |
9.0 ± 3.5 |
9.0 ± 2.0 |
13.3 ± 3.2 |
333 |
103.3 ± 6.0 |
28.0 ± 11.3 |
9.0 ± 2.6 |
7.0 ± 1.0 |
15.0 ± 1.0 |
1000 |
94.7 ± 8.6 |
24.0 ± 1.0 |
7.3 ± 2.3 |
3.3 ± 1.5 |
12.7 ±4.5 |
2500 |
82.7 ± 15.0 |
10.0 ± 4.0 |
6.0 ± 3.6 |
3.7 ± 2.1 |
15.7 ± 1.5 |
5000 |
85.0 ± 5.6 |
8.3 ± 2.5 |
7.7 ± 1.5 |
4.3 ± 2.5 |
17.7 ± 6.1 |
MNNG (5) |
4149.7 ± 147.0 |
- |
4984.7 ± 175.6 |
- |
- |
NOPD (10) |
- |
909.7 ± 30.1 |
- |
- |
- |
AAC (100) |
- |
- |
- |
1997.7 ± 133.0 |
- |
4-NQO (5) |
- |
- |
- |
- |
1130.3 ± 48.2 |
|
|
||||
|
Results with S9 |
||||
DMSO |
112.0 ± 16.0 |
25.7 ± 5.5 |
9.0 ± 1.7 |
9.7 ± 3.5 |
26.3 ± 8.5 |
33 |
101.7 ± 7.5 |
28.3 ± 9.1 |
7.7 ± 2.3 |
12.0 ± 4.0 |
23.0 ± 9.6 |
100 |
95.3 ± 4.7 |
29.3 ± 6.7 |
8.0 ± 3.6 |
11.0 ± 2.6 |
25.0 ± 4.6 |
333 |
92.0 ± 27.6 |
28.7 ± 1.5 |
10.3 ± 2.1 |
7.7 ± 4.0 |
29.7 ± 8.1 |
1000 |
78.7 ± 7.5 |
22.7 ± 4.5 |
8.7 ± 2.1 |
6.7 ± 2.1 |
25.0 ± 1.7 |
2500 |
68.3 ± 8.4 |
12.7 ± 2.1 |
5.3 ± 0.6 |
6.0 ± 2.6 |
17.0 ± 3.6 |
5000 |
109.0 ± 14.2 |
21.7 ± 7.2 |
8.3 ± 2.1 |
8.3 ± 4.2 |
18.7 ± 3.5 |
2- AA (2.5) |
1467.3 ± 116.7 |
1234.3 ± 41.5 |
149.3 ± 12.4 |
118.7 ± 3.8 |
93.3 ± 21.2 |
Table 2. Experiment 2 Pre-incubation test
Dose (µg/plate) |
Mean number of revertant colonies of 3 replicates (± S.D.) for different strains of S. typhimurium and E. coli |
||||
|
TA 100 |
TA 98 |
TA 1535 |
TA 1537 |
WP2 uvrA |
|
|
||||
|
Results without S9 |
||||
DMSO |
94.7 ± 6.1 |
20.3 ± 6.4 |
15.3 ± 3.8 |
9.7 ± 3.1 |
20.0 ± 7.5 |
33 |
84.0 ± 3.5 |
19.0 ± 4.4 |
7.7 ± 1.2 |
9.0 ± 2.0 |
14.3 ± 2.5 |
100 |
92.0 ± 3.6 |
15.0 ± 3.6 |
7.7 ± 2.9 |
7.7 ± 2.1 |
13.7 ± 3.1 |
333 |
90.7 ± 8.1 |
18.0 ± 2.6 |
10.3 ± 6.5 |
11.0 ± 3.0 |
14.0 ± 2.6 |
1000 |
84.7 ± 12.9 |
13.0 ± 5.3 |
8.7 ± 2.1 |
11.7 ± 4.0 |
15.0 ± 5.6 |
2500 |
90.3 ± 14.6 |
17.7 ± 1.2 |
7.0 ± 2.6 |
11.0 ±4.4 |
13.0 ± 6.1 |
5000 |
76.0 ±8.7 |
13.0 ± 4.0 |
8.7 ± 1.5 |
6.3 ± 2.5 |
13.3 ± 3.1 |
MNNG (5) |
2189.0 ± 191.8 |
- |
2491.7 ± 96.4 |
- |
- |
NOPD (10) |
- |
811.0 ±41.4 |
- |
- |
- |
AAC (100) |
- |
- |
- |
1201.7 ± 148.5 |
- |
4-NQO (5) |
- |
- |
- |
- |
322.0 ± 18.1 |
|
|
||||
|
Results with S9 |
||||
DMSO |
124.0 ±16.7 |
22.3 ± 3.6 |
12.0 ± 3.5 |
11.0 ± 5.3 |
22.0 ± 3.6 |
33 |
71.3 ± 1.2 |
16.7 ± 3.8 |
8.7 ± 1.2 |
6.7 ± 3.8 |
16.7± 3.8 |
100 |
76.0 ± 8.0 |
16.0 ± 1.0 |
9.0 ± 4.4 |
9.0 ± 1.7 |
16.0 ± 1.0 |
333 |
67.0 ± 2.6 |
17.0 ± 2.6 |
8.0 ±4.6 |
10.3 ± 3.1 |
17.0 ± 2.6 |
1000 |
73.3 ± 18.5 |
14.7 ± 4.2 |
9.0 ± 4.6 |
9.0 ± 0.0 |
14.7 ± 4.2 |
2500 |
73.3 ± 8.3 |
16.7 ± 3.1 |
8.3 ± 2.3 |
9.0 ± 1.0 |
16.7 ± 3.1 |
5000 |
82.0 ± 11.3 |
14.7 ± 4.6 |
4.3 ± 0.6 |
5.3 ± 1.5 |
14.7 ± 4.6 |
2- AA (2.5) |
1187.7 ± 57.6 |
111.7 ± 6.4 |
167.7 ± 10.7 |
156.7 ±17.6 |
111.7 ± 6.4 |
Table 3. Decreased revertant numbers (cytotoxicity) observed at following concentrations (µg/plate)
Experiment |
S9 |
TA 1535 |
TA 100 |
TA 1537 |
TA 98 |
E. coli |
1st - SPT |
Without |
- |
- |
1000 - 5000 |
2500 - 5000 |
- |
With |
2500 |
|||||
2nd - PIT |
Without |
1000 - 5000 |
- |
- |
5000 |
- |
With |
5000 |
33 - 2500 |
5000 |
- |
- |
Table 4. Historical control data
|
|
Bacterial strains |
|||||
Historical control data of DMSO control |
-S9 |
|
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
E. coli |
Average |
18 |
95 |
10 |
7 |
22 |
||
SD |
3.1 |
9.9 |
2.2 |
1.5 |
4.0 |
||
Minimum |
12 |
69 |
6 |
5 |
12 |
||
Maximum |
30 |
140 |
17 |
12 |
33 |
||
+S9 |
|
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
E. coli |
|
Average |
24 |
100 |
10 |
8 |
23 |
||
SD |
4.7 |
12.3 |
2.1 |
2.2 |
4.0 |
||
Minimum |
13 |
76 |
6 |
5 |
13 |
||
Maximum |
35 |
150 |
18 |
16 |
35 |
||
Historical control data of positive control |
-S9 |
|
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
E. coli |
Average |
486 |
3331 |
4063 |
975 |
884 |
||
SD |
183.3 |
1021.5 |
1308.8 |
339.9 |
442.4 |
||
Minimum |
271 |
1125 |
1174 |
253 |
133 |
||
Maximum |
431 |
5557 |
6612 |
1858 |
1552 |
||
+S9 (2-AA |
|
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
E. coli |
|
Average |
1427 |
1792 |
217 |
130 |
98 |
||
SD |
361.9 |
448.9 |
50.0 |
33.6 |
26.6 |
||
Minimum |
431 |
361 |
108 |
56 |
54 |
||
Maximum |
2427 |
2819 |
367 |
241 |
169 |
Applicant's summary and conclusion
- Conclusions:
- Under the experimental conditions chosen here, it is concluded that the test article is not a mutagenic test substance in the bacterial reverse mutation test in the absence and the presence of metabolic activation.
- Executive summary:
The test substance was tested for its mutagenic potential based on the ability to induce point mutations in selected loci of several bacterial strains in a reverse mutation assay. Salmonella typhimurium TA 1535, TA 100, TA 1537, TA 98 and E. coli WP2 uvrA were used as tester strains with a dose range of 33 μg - 5000 μg/plate in both standard plate test and preincubation test with and without metabolic activation (liver S9 mix from induced rats). Precipitation of the test substance was found from about 333 μg/plate onward with and without S9 mix. A weak bacteriotoxic effect was observed depending on the strain and test conditions from about 33 μg/plate onward. A relevant increase in the number of his+ or trp+ revertants (factor ≥ 2: TA 100, TA 98 and E.coli WP2 uvrA or factor ≥ 3: TA 1535 and TA 1537) was not observed in the standard plate test or in the preincubation test without S9 mix or after the addition of a metabolizing system. Under the experimental conditions of this study, the test substance GSID 1212253 is not mutagenic in the Salmonella typhimurium/Escherichia coli reverse mutation assay in the absence and the presence of metabolic activation.
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