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Diss Factsheets
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EC number: 918-045-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005-06-03 to 2005-12-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 5-[2-ethoxy-5-(4-methylpiperazin-1-yl)sulfonylphenyl]-1-methyl-3-propyl-4H-pyrazolo[4,3-d]pyrimidin-7-one;2-hydroxypropane-1,2,3-tricarboxylic acid
- Cas Number:
- 171599-83-0
- Molecular formula:
- C28H38N6O11S
- IUPAC Name:
- 5-[2-ethoxy-5-(4-methylpiperazin-1-yl)sulfonylphenyl]-1-methyl-3-propyl-4H-pyrazolo[4,3-d]pyrimidin-7-one;2-hydroxypropane-1,2,3-tricarboxylic acid
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material:
- Expiration date of the lot/batch: November 1, 2005
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient
- Stability under test conditions: Stable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The contents of the first control vessel were transferred to a BOD bottle and dissolved oxygen concentrations were measured every 10 seconds over a 10 minute period, or until dissolved oxygen concentrations fell below 1.0 mg/L, using a YSI Model 50B Dissolved Oxygen Meter. The respiration rate in subsequent vessels was determined in an identical manner at 15 minute intervals.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct weight addition
- Controls: A positive control with a known respiration inhibitor was used.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Water
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)):
The reference group was dosed with 3,5-dichlorophenol, a known inhibitor of respiration, at concentrations of 3, 15 and 50 mg/L.
The test substance was tested at nominal concentrations of 1, 3, 10, 30, 100, 300, and 1000 mg/L.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): The test substance dissolved poorly in the water.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Denton Wastewater Treatment Plant, Denton, Maryland on June 27, 2005.
- Preparation of inoculum for exposure: The sludge was sieved using a 2 mm screen and allowed to settle for approximately 30 minutes. The supernatant above the settled solids was removed.
- Pretreatment: Total suspended solids in the settled sludge were adjusted to by dilution with municipal water. 50 mL of synthetic sewage was added to each liter of adjusted sludge.
- Initial biomass concentration: A nominal concentration of approximately 4000 mg/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- Control, reference, and treatment test mixtures were incubated at 20 ± 2ºC and aerated for three hours at a rate sufficient to provide aerobic conditions and maintain solids in suspension.
- Post exposure observation period:
- No post exposure observation period was conducted.
Test conditions
- Test temperature:
- 20 ± 2ºC
- pH:
- The measured initial pH of the sludge on the day of testing was 8.3 and was adjusted to 7.6 prior to use in the test system.
- Nominal and measured concentrations:
- Nominal concentrations:
- Concentration of Inncoulum: 4000 mg/L
- Concentrations of Positive Control: 3, 15 and 50 mg/L
- Concentrations of Test Substance: 1, 3, 10, 30, 100, 300, and 1000 mg/L
Measured concentrations:
- Concentration of Inncoulum: 4393 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 ml plastic Erlenmeyer flask
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 300 mL was filed
- Aeration: Yes, at a rate sufficient to provide aerobic conditions and maintain solids in suspension.
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Sludge concentration (weight of dry solids per volume): 4393 mg/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water
OTHER TEST CONDITIONS
- Adjustment of pH: The measured initial pH of the sludge on the day of testing was 8.3 and was adjusted to 7.6 prior to use in the test system
- Details on termination of incubation: Upon terminitation, smaples weretransferred into 300 mL biochemical oxygen demand (BOD) bottles to conduct dissolved oxygen (DO) measurements
EFFECT PARAMETERS MEASURED:
DO measurements were measured every 10 seconds over a 10 minute period for the first control vessel.
The respiration rate in subsequent vessels was determined in an identical manner at 15 minute intervals.
TEST CONCENTRATIONS
- Concentrations of Positive Control: 3, 15 and 50 mg/L
- Concentrations of Test Substance: 1, 3, 10, 30, 100, 300, and 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol at 99.1% purity at concentration of 3, 15 and 50 mg/L.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Details on results:
- The observed percent inhibitions for sildenafil citrate ranged from –53.6 to 15.7% and were not concentration dependent.
- Results with reference substance (positive control):
- The positive control EC50 had 95 percent confidence limits of 23 and 29 and was within the 5 to 30 mg/L range considered acceptable for the test. This makes the result of this test valid.
- Reported statistics and error estimates:
- The EC50 was calculated using the moving average method.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- This study accurately assesed the EC50 and NOEC for respiration inhibition in activated sludge per guidelines.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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