Bromo(hexahydro-2H-azepin-2-onato-N)magnesium

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01/1999 to 02/1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Bromo(hexahydro-2H-azepin-2-onato-N)magnesium

EC Number:

241-158-2

EC Name:

Bromo(hexahydro-2H-azepin-2-onato-N)magnesium

Cas Number:

17091-31-5

Molecular formula:

C6H10BrMgNO

IUPAC Name:

magnesium(2+) ion 2-oxoazepan-1-ide bromide

additive 1

Reference substance name:

ε-caprolactam

EC Number:

203-313-2

EC Name:

ε-caprolactam

Cas Number:

105-60-2

Molecular formula:

C6H11NO

IUPAC Name:

azepan-2-one

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 181298

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

not required

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS strain Berlin

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Hardness:

calcium 28.9 mq/L
magnesium 4.9 mg/L

Test temperature:

20 ± 2 ·c

pH:

8.19 to 9.78

Dissolved oxygen:

9.36 to 9.46 mg/l

Salinity:

not specified

Conductivity:

not specified

Details on test conditions:

Using a glass tube, the daphnia were caught and lifted from the 250 ml beaker.
They were put on a small sieve, and the medium surrounding the animals was
sucked off using absorbent paper. Immediately after that, the animals were put into
the respective test solution.
The test vessels were left to stand for 24 hours without feeding or lighting. After 24
hours, the immobilised daphnia were counted. Daphnia are considered immobilised if
they perform no own movements or are only able to move their antennae when the
beaker is gently agitated.
The pH and the concentration of dissolved oxygen in the test vessels were measured
at the beginning and at the end of the test.
All dates and values were documented in the raw data.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

3.63 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

LOEC

Effect conc.:

2.5 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

2 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

8 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The 24 h EC50 value for the test item was determined to be 3.63 g/l in a short-term toxicity test to daphnia magna.