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EC number: 230-589-1 | CAS number: 7209-38-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 1979 - January 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- N,N'-bis(3-aminopropyl)piperazine
- EC Number:
- 230-589-1
- EC Name:
- N,N'-bis(3-aminopropyl)piperazine
- Cas Number:
- 7209-38-3
- Molecular formula:
- C10H24N4
- IUPAC Name:
- 3-[4-(3-aminopropyl)piperazin-1-yl]propan-1-amine
- Test material form:
- liquid
- Details on test material:
- - name (as stated in the report): N,N'-Bis-aminopropyl-piperazin
- chemical name: 1,4-Bis-(aminopropyl)-piperazin
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- - Fasting period before study: 15 - 20 hours
- Diet: Herilan MRH-Haltung, Eggersmann KG
- Weight at study initiation: males: 140 - 210 g, females: 160 - 190 g
Administration / exposure
- Route of administration:
- oral: unspecified
- Vehicle:
- water
- Details on oral exposure:
- Substance was diluted in water
- Doses:
- 5000 mg/kg bw (50 % dilution); 3160 mg/kg bw (31.6 %); 2150 mg/kg bw (21.5 %); 1470 mg/kg bw (14,7 %); 1000 mg/kg bw (10 %)
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- The test compound was administered orally to 5 groups of 10 rats (5 male, 5 female) fasted for 15 - 20 hours prior to dosing. The animals were observed during the day of dosing and daily thereafter for 14 days. Decents during the study were examined for gross lession.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 1 980 mg/kg bw
- 95% CL:
- >= 1 694 - <= 2 330
- Mortality:
- 1000 mg/kg bw: 0/5 (females) and 0/5 (males)
1470 mg/kg bw: 1/5 (females) and 0/5 (males), The decedent was found within 1 day post-treatment.
2150 mg/kg bw: 4/5 (females) and 2/5 (males), The male decedents were found within 1 day post-treatment. 3 female decedents were found within 1 day post-treatment, the other one on the second day.
3160 mg/kg bw: 5/5 (females) and 5/5 (males), The decedents were found within 1 day post-treatment.
5000 mg/kg bw: 5/5 (females) and 5/5 (males), 2 female decedents were found after the first hour post-treatment. The rest were found within 1 day post-treatment. - Clinical signs:
- other: 1000 mg/kg bw: no clinical signs 1470 mg/kg bw: dyspnea, apathy, hunched posture, uncoordinated movements, atony, disrupted nociceptive reflex, coma, spastic gait, strongly yellow urine, shaggy fur, cyanose, bad general condition 2150 mg/kg bw: dyspnea, a
- Gross pathology:
- dead rats:
heart: acute dilatation, hyperaemia
stomach: diffuse redness of gastric mucosa
intestine: diffuse redness of intestinal mucosa
liver: enlarged liver, confluent clay-coloured lobular pattern
killed rats: no findings
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- LD50: 1980 mg/kg/bw
EU CLP / UN GHS: Acute toxicity category 4: harmful if swallowed - Executive summary:
N,N'-bis-(3 -aminopropyl)piperazine was administered orally to 5 groups of 10 rats (5 male, 5 female). The substance was delivered as formulation in water in 5 different concentrations 50 % (5000 mg/kg bw), 31.6 % (3160 mg/kg bw), 21.5 % (2150 mg/kg bw), 14.7 % (1470 mg/kg bw), 10 % (1000 mg/kg bw). All animals were subjected to daily observations and weekly determination of body weight. Macroscopic examination was performed on the day of death or after terminal sacrifice (Day 14).
The body weight gain shown by the surviving animals over the study period was considered to be normal.
Macroscopic post mortem examination of the animals found dead during the study revealed abnormalities in the heart (acute dilatation, hyperaemia), stomach (diffuse redness of gastric mucosa), intestine (diffuse redness of intestinal mucosa), liver (enlarged liver, confluent clay-coloured lobular pattern. No abnormalities were revealed in the surviving animals.
The oral LD50 value of N,N'-bis(3 -aminopropyl)piperazine in rats was established to be 1980 mg/kg/bw.
Based on these results:
- according to the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) of the United Nations, N,N'-bis(3 -aminopropyl)piperazine should be classified as: harmful if swallowed (Category 4) for acute toxicity by the oral route.
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