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EC number: 200-837-3 | CAS number: 75-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study is classified as reliable with restrictions because it was well conducted and documented and generally follows OECD Guideline 420.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 958
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 420 (Acute Oral Toxicity - Fixed Dose Method)
- Deviations:
- yes
- Remarks:
- maximum dose exceeded guideline recommended dose; animal fasting was not reported; animals were not weighed; pathology and clinical observations were generalized across dose levels of all chemicals tested
- GLP compliance:
- no
- Test type:
- fixed dose procedure
- Limit test:
- no
Test material
- Reference substance name:
- Ethanethiol
- EC Number:
- 200-837-3
- EC Name:
- Ethanethiol
- Cas Number:
- 75-08-1
- Molecular formula:
- C2H6S
- IUPAC Name:
- ethanethiol
- Details on test material:
- - Name of test material (as cited in study report): Ethanethiol (ethyl mercaptan)
- Analytical purity: gas chromatographic analysis showed the chemical to be essentially pure
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: local commercial breeder
- Weight at study initiation: 180 to 220 grams
- Diet (e.g. ad libitum): Rockland Rat Diet ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: one week
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- VEHICLE : No vehicle used
MAXIMUM DOSE VOLUME APPLIED: 3360 mg/kg bw
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: doses were at geometric progression ( factor 1.26 to 2.0) - Doses:
- 210, 420, 840, 1680, and 3360 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 15 days
- Frequency of observations and weighing: Observed on days 1, 2, 3, 5, 10, and 15 no weighing was conducted
- Necropsy of survivors performed: yes
- Other examinations performed: pathology - Statistics:
- LD50 calculated by the method of Weil (Weil, C.S.: Tables for Convenient Calculation of Median-Effective Dose (LD50 or ED50) and Instruction in Their Use. Biometrics, 8: 249-304 (1954).)
Results and discussion
- Preliminary study:
- No data reported
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 682 mg/kg bw
- 95% CL:
- 517 - 900
- Mortality:
- Mortality occurred in all animals by 7 hours of administration for the 3360 mg/kg dose and day 5 for the 1680 mg/kg dose. Four of the five animals died in the 840 mg/kg dose level; and no mortality occurred at the 420 and 210 dose levels.
- Clinical signs:
- Toxicity signs included sedative action, and maximal sublethal doses resulted in deep comatose sleep for approximately 48 hours. Diarrhea was also pronounced for the highest doses.
- Body weight:
- No data reported
- Gross pathology:
- Gross pathology generally did not show significant gross or microscopic tissue changes. All animals that survived near lethal doses and were sacrificed within 20 days post-treatment, frequently showed pathologic changes which, although inconsistent, were indicative of liver and kidney damage. Microscopic examination revealed occasional marked changes in the kidneys of rats which included: degeneration with swelling and some necrosis of the tubular epithelium, thickening of Bowman’s capsule, and hyaline deposition in glomerular tufts. More often only minor lesions with varying degrees of cloudy swelling of the tubules and hyaline casts in the lumina were present. In general, liver changes were characterized by lymphocytic infiltration, occasional necrotic foci with small hemorrhages, and varying degrees of fatty degeneration. Only rarely did tissue studies show significant pathologic conditions as the result of relatively small doses of the chemical.
- Other findings:
- No data reported
Any other information on results incl. tables
Toxicity Data for Rats Following Single Oral Dose of Ethanethiol
Dose (mg/kg) |
Cumulative Mortality following Administration for the Day |
|||||
1 |
2 |
3 |
5 |
10 |
15 |
|
210 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
420 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
840 |
2/5 |
2/5 |
2/5 |
2/5 |
3/5 (7th) |
4/5 (11th) |
1680 |
4/5 |
4/5 |
4/5 |
5/5 |
|
|
3360 |
5/5 (all dead 4 to 7 hrs) |
|
|
|
|
|
LD50(mg/kg) |
1034 |
|
|
|
|
682 |
Confidence Limits |
667-1603 |
|
|
|
|
517-900 |
Applicant's summary and conclusion
- Interpretation of results:
- Toxicity Category IV
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- In an acute oral toxicity study, groups of Wistar male rats (5/sex) were given a single oral dose of ethanethiol undiluted at doses of 210, 420, 840, 1680, or 3360 mg/kg bw and observed for 15 days (Fairchild & Stokinger, 1958). The oral LD50 was determined to be 682 mg/kg bw in males. This study is classified as Toxicity Category IV by EU classification.
- Executive summary:
In an acute oral toxicity study, groups of Wistar male rats (5/sex) were given a single oral dose of ethanethiol undiluted at doses of 210, 420, 840, 1680, or 3360 mg/kg bw and observed for 15 days (Fairchild & Stokinger, 1958).
Toxicity signs included sedative action, and maximal sublethal doses resulted in deep comatose sleep for approximately 48 hours. Diarrhea was also pronounced for the highest doses. Gross pathology generally did not show significant gross or microscopic tissue changes. Survivors of near lethal doses showed changes which, although inconsistent, were indicative of liver and kidney damage. Body weights were not measured. The oral LD50 was determined to be 682 mg/kg bw in males. This study is classified as Toxicity Category IV by EU classification.
This study received a Klimisch score of 2 and is classified as reliable with restrictions because it was well conducted and documented and generally follows OECD Guideline 420.
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