4,7-dichloroquinoline

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of test chemicals

Justification for type of information:

data from handbook or collection of data

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE was prepared for the determination of effect of test chemical on the growth rate of microorganisms.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: WoE - 2: Tetrahymena thermophila, WoE - 3: Photobacterium phosphoreum

Details on inoculum:

WoE - 2:
Species Latin Name: Tetrahymena thermophila
Species Common Name: Ciliate Protozoa
Organism Life Stage: Stationary growth phase
Organism Characteristics: 10000 CELLS/ML

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

15 minutes exposure in WoE 3

Test temperature:

WoE - 2: 32 °C

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

25.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Effects were observed on population growth rate inhibition

Remarks:

WoE - 2

Duration:

15 min

Dose descriptor:

EC50

Effect conc.:

3.29 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Inhibition of luminescence

Remarks on result:

other: WoE - 3

Validity criteria fulfilled:

not specified

Conclusions:

As the study was conducted on two different organisms, effects were measured on the basis of different parameter. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l. Also based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical
exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l. Thus on the basis of above all studies, the EC50 value ranges from 3.29 to 25.5 mg/l.

Executive summary:

Based on the various experimental studies from different sources, studies were reviewed for the determination of effects of test chemical on the growth of microorganisms and the studies are as mention below:

Principle of this study from secondary source was to determine the effect of test chemical on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa). Test conducted under the static system for 48 hours. After the exposure period of 48 hours, effects on the population growth was observed and noted. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l.

Above study further supported by the weight of evidence data from secondary sources. Principle of this study was to determine the effect of test chemical on the luminescence of Photobacterium phosphoreum. Test conducted for 15 minutes. Based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l.

As the study was conducted on two different organisms, effects were measured on the basis of different parameter. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l. Also based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l. Thus on the basis of above all studies, the EC50 value ranges from 3.29 to 25.5 mg/l.

Description of key information

As the study was conducted on two different organisms, effects were measured on the basis of different parameter. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l. Also based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l. Thus on the basis of above all studies, the EC50 value ranges from 3.29 to 25.5 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

25.5 mg/L

Additional information

Based on the various experimental studies from different sources, studies were reviewed for the determination of effects of test chemical and structually and functionally similar read across chemicals on the growth of microorganisms and the studies are as mention below:

Principle of this study from secondary source was to determine the effect of test chemical on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa). Test conducted under the static system for 48 hours. After the exposure period of 48 hours, effects on the population growth was observed and noted. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l.

Above study further supported by the weight of evidence data from secondary sources. Principle of this study was to determine the effect of test chemical on the luminescence of Photobacterium phosphoreum. Test conducted for 15 minutes. Based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l.

As the study was conducted on two different organisms, effects were measured on the basis of different parameter. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l. Also based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l. Thus on the basis of above all studies, the EC50 value ranges from 3.29 to 25.5 mg/l.