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EC number: 426-840-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Start of Experiments: November 12, 1996 - Completion of Experiments: December 19, 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted April 04, 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- dated December 29, 1992
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- For the analysis of the actual test item concentrations the following samples were taken:
Just before test start:
- duplicate samples from the test medium (without daphnids)
- duplicate samples from the control (without daphnids}
After 48 hours (stability samples) :
- duplicate samples from the test medium
- duplicate samples from the control
For the 48-hour stability samples the contents of the two respective test beakers were combined.
The 0-hour samples were deep-frozen (at about -20 °C) immediately after sampling. Based on pre-experiments for investigation of the storage stability (without GLP) the test item is sufficiently stable in the test water under these storage conditions. The 48-hour samples were analyzed immediately after sampling.
The concentration of the test item SCARLET RN 1165 was analyzed in the duplicate test medium samples of the single test concentration for both sampling times (0 and 48 hours).
From the control only one of the duplicate samples was analyzed from each of both sampling times. - Vehicle:
- no
- Details on test solutions:
- The test medium with the test substance concentration of nominal 100 mg/l was prepared by dissolving the test substance homogeneously in test water by intense stirring. The test medium was freshly prepared just before the start of the test(= introduction of Daphnia).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: supplied by the Ruhrverband, Essen, F.R.G. in 1989
- Feeding during test:
- Food type:
- Amount:
- Frequency:
ACCLIMATION
- Acclimation conditions (same as test or not): The clone was bred in the laboratories of RCC under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: - Test type:
- static
- Water media type:
- other: Reconstituted test water
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- None
- Hardness:
- 2.5 mmol/l (= 250.0 mg/I) as CaC03.
- Test temperature:
- 20.7 - 20.8°C during the test period.
- pH:
- During the test period, the pH-values ranged from pH 7.7 to pH 8.0.
- Dissolved oxygen:
- During the test period, the dissolved oxygen concentrations in the test media were at least 8.0 mg/l or higher.
- Salinity:
- CaCl2 x2H20 : 2.0 mmol/L (= 294.0 mg/L)
MgS04 x 7H20 : 0.5 mmol/L (= 123.0 mg/L)
NaHC03 : 0.75 mmol/L (= 65.0 mg/L)
KCI : 0.075 mmol/L (= 5.8 mg/L) - Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal: 100 mg/L
The analytical determined test substance concentrations in the test medium of nominal 100 mg test substance/I varied in the range of 91 % to 94 % of the nominal value during the test period. The mean measured concentration (calculated as the average over all measurements per concentration) was 92 % of nominal.
Therefore, all reported results are related to the nominal concentration of the test substance. - Details on test conditions:
- TEST SYSTEM
In each treatment, the test concentration and the control, 20 Daphnia were tested, divided into four groups of five animals, each group in 50 ml test medium in a 100-ml-glassbeaker.
The test was performed in an air-temperated room.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Reconstituted water: in deionized water analytical grade salts were added to following nominal concentrations
CaCl2 x2H20 : 2.0 mmol/L (= 294.0 mg/L)
MgS04 x 7H20 : 0.5 mmol/L (= 123.0 mg/L)
NaHC03 : 0.75 mmol/L (= 65.0 mg/L)
KCI : 0.075 mmol/L (= 5.8 mg/L)
Water Hardness : 2.5 mmol/L (=250.0 mg/L) as CaC03
Alkalinity : 0.8 mmol/L
Ratio of Ca : Mg = 4 : 1 (based on molarity)
Na : K = 1 O : 1 (based on molarity)
The test water was aerated to oxygen saturation.
OTHER TEST CONDITIONS
- Photoperiod: A 16-hour light to 8-hour darkness photoperiod
- Light intensity: light intensity at light period approximatively 400-550 Lux
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- In the control and in the test concentration of nominal 100 mg test substance/I no immobility or mortality of the test animals was observed during the test period of 48 hours.
Therefore, the 48-hour NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours), and the 48-hour ECO of SCARLET RN 1165 to Daphnia magna were determined to be at least 100 mg test substance/I. The NOEC and the EC0 might even be higher than this concentration, but concentrations in excess of 100 mg test substance/I have not been tested according to the EEC Commission Directive. The 48-hour LOEC (lowest concentration with toxic effects) and the 48-hour EC 50 were clearly higher than 100 mg test substance/l. These values could not be quantified due to the absence of toxicity of SCARLET RN 1165 up to this test concentration. - Results with reference substance (positive control):
- Positive control not used.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test item BLUE GS 5664.80 to Daphnia magna was determined in a 48-hour static test according to the EU Commission Directive 92/69/EEC, Part C.2 (1992), and the OECD Guideline for Testing of Chemicals No. 202, (1984), resulting in a 48-hour EC50 > 100 mg/L.
- Executive summary:
The acute toxicity of the test substance SCARLET RN 1165 to Daphnia magna was determined in a 48-hour static test according to the Commission Directive 92/69/EEC, Annex Part C.2, dated December 29, 1992, and the OECD Guideline for Testing of Chemicals, Section 2, No. 202, Part I, adopted April 04, 1984.
A limit test was performed in accordance with the Commission Directive 92/69/EEC to demonstrate that the test substance has no toxic effect on the test animals up to the concentration of nominal 100 mg test substance/l. Thus the only concentration tested was nominal 100 mg test substance/l and a control.
The analytical determined test substance concentrations in the test medium of nominal 100 mg test substance/l varied in the range of 91 % to 94 % of the nominal value during the test period. The mean measured concentration (calculated as the average over all measurements per concentration) was 92 % of nominal. Therefore, all reported results are related to the nominal concentration of the test substance.
The 48-hour NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours), and the 48-hour EC0 of SCARLET RN 1165 to Daphnia magna were determined to be at least 100 mg test substance/I. The NOEC and the EC0 might even be higher than this concentration, but concentrations in excess of 100 mg test substance/l have not been tested. The 48-hour LOEC (lowest concentration with toxic effects) and the 48- hour EC 50 were clearly higher than 100 mg test substance/I. These values could not be quantified due to the absence of toxicity of SCARLET RN 1165 up to this test concentration.
Reference
Description of key information
The acute toxicity of the test item BLUE GS 5664.80 to Daphnia magna was determined in a 48-hour static test according to the EU Commission Directive 92/69/EEC, Part C.2 (1992), and the OECD Guideline for Testing of Chemicals No. 202, (1984), resulting in a 48-hour EC50 > 100 mg/L.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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