α,α,α-trichlorotoluene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The authors tested acute fish toxicity on Leuciscus idus melanotus with the Golden Orfe test according to DIN 38412 Part 15. No GLP standards are specified but sufficient data are given concerning the materials and methods and the results. Therefore, the study should be considered as reliable with restrictions, a Klimisch 2d study, a test procedure in accordance with national standards with acceptable restrictions.

Data source

Reference

Reference Type:

publication

Title:

Ergebnisse der Untersuchung von 200 chemischen Verbindingen auf akute Fischtoxizität mit dem Goldorfentest.

Author:

Juhnke I. and Lüdemann D.

Year:

1978

Bibliographic source:

Z. Wasser- und Abwasser-Forschung 11: 161-164

Materials and methods

Principles of method if other than guideline:

Test performed according to DIN 38412 Part 15.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

α,α,α-trichlorotoluene

EC Number:

202-634-5

EC Name:

α,α,α-trichlorotoluene

Cas Number:

98-07-7

Molecular formula:

C7H5Cl3

IUPAC Name:

α,α,α-trichlorotoluene

Constituent 2

Reference substance name:

trichloromethylbenzene

IUPAC Name:

trichloromethylbenzene

Details on test material:

- Name of test material (as cited in study report): benzotrichlorid

No more data available

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

2 480 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

4 140 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

8 280 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

The authors tested acute fish toxicity of benzotrichloride on Leuciscus idus melanotus with the Golden Orfe test according to DIN 38412 Part 15. Under the test conditions, a LC50 after 48h was reported to be 4140 mg/L.
At this level of information, the test substance can not be classified according to the CLP regulation (EC) n° 1272/2008 since the basis of classification is the LC50 value after 96h.

Executive summary:

The authors tested acute fish toxicity of benzotrichloride (CAS n° 98-07-7) on Leuciscus idus melanotus with the Golden Orfe test according to DIN 38412 Part 15. Few details are given concerning the materials and methods.

Under the test conditions, a LC50 after 48h was reported to be 4140 mg/L. At this level of information, the test substance can not be classified according to the CLP regulation (EC) n° 1272/2008 since the basis of this classification is the LC50 value after 96h.

Besides, even if it is not clearly stated whether the incubation solutions were neutralized before incubation, the concentration reached at LC0 would involve such big acidification of the test medium (pH 1 -2 ), it would kill all fishes. Hence, as no mortality was observed at this level, it can be assumed that the test solutions were neutralized before start of incubation as required by the standard. Other details on the requirements of the standard can not be controlled since only main elements of the experiments are reported.

Hence, no GLP standards are specified and sufficient data are given concerning the materials and methods and the results. Therefore, the study should be considered as reliable with restrictions, a test procedure in accordance with national standards with acceptable restrictions.