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EC number: 946-009-1 | CAS number: 91722-69-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-06-12 to
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP study performed according to OECD Guideline No. 201. All validity criteria were fulfilled.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- signed on January 10, 2017
- Specific details on test material used for the study:
- Not applicable
- Analytical monitoring:
- yes
- Details on sampling:
- Single samples for analysis were taken from the control and all test concentrations (from a replicate of each test concentration without algae dedicated exclusively to chemical analyses) at the start and the end of the test. Concentration of dissolved organic material was checked by analysis of Total Organic Carbon (TOC) in the control medium and the WAFs. TOC analysis was not performed in compliance with the OECD GLP principles but was adapted to fit the specific parameters of the test item, in accordance with ISO 17025.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The study was carried out using WAFs (Water Accommodated Fractions). The WAFs were prepared under closed conditions and by slow-stirring. The mixing vessels were 1 L cylindrical glass bottles sealed with screw caps and fitted with a drain port near the bottom for drawing off the WAFs. A magnetic stirring bar was placed in each mixing vessel completely filled with test water (with a minimum of headspace). The loading rates of the test item were weighed in glass flasks (approximate volume: 100 mL) filled with minimum headspace with test water (from the mixing vessel) and were immediately sealed with screw caps after weighing. Each glass flask was placed in a water bath for 10 minutes at 50°C, followed by sonication for approx. 10 minutes. Based on experience on similar substances, these steps (heating and sonication) were essential to remove the paste fragments stuck to the glass of the flasks and to extract the soluble fraction of the test item as much as possible. Then the mixing vessels were carefully filled with the contents of the glass flasks and thereafter were closed immediately. The mixing was initiated with the vortex in the centre extending maximally around 10% vessel depth from the top to the bottom of the vessel. After 23 hours of gentle stirring in the dark at room temperature, the WAFs were allowed to stand for at least 1 hour before use. The first 100 mL were removed via the drain port. Then the WAFs were directly added into test flasks containing a fixed amount of inoculum (5.103 cells.mL-1 per vessel) that were immediately sealed after filling with a minimum of headspace. At the start of the test, the solution was observed to be clear and colourless.
- Controls: Test water without test substance but treated in the same way as the test substance solutions (WAFs). - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Museum National d’Histoire Naturelle - 12, rue Buffon, Case N°19 -75005 PARIS, bred in the Laboratoires des Pyrénées et des Landes under standardised conditions according to the test guidelines.
- Stock culture: Algae stock cultures were started by inoculating growth medium (=test water) with algal cells from a pure culture on agar. The suspensions were continuously aerated and exposed to light in a climate room at a temperature of 23 ± 2 °C.
- Pre-culture: 2 to 4 days before the start of the test, cells from the algal stock culture were inoculated in test water at a cell density of 1 x 10^4 cells/mL. The pre-culture was maintained under the same conditions as used in the test. The cell density was measured immediately before use. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- None
- Hardness:
- No data
- Test temperature:
- 22.8-23.0 °C throughout the test (average value: 22.9 °C)
- pH:
- Start(t=0 h): 8.30 - 8.44
End (t=72 h): 8.88 - 10.42 - Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations: 10.0; 17.7; 31.6; 56.2 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, all-glass closed flasks with ground glass stopper, completely filled with test solution with minimum headspace.
Each test vessel was uniquely identified with study code, replicate number, date of experimentation and treatment group.
- Initial cells density: An initial cell density of 5.10^3 cells.mL-1 using the exponentially growing pre-culture.
- Replicates: 6 controls and 3 replicates of each loading rate for counting. Moreover, a replicate of each treatment without algae was prepared for chemical analyses.
GROWTH MEDIUM
- Standard medium used: Yes; Original medium of OECD TG 201. Since the test was performed in sealed conditions, additional sodium bicarbonate was added to test water (for all treatments and inoculum suspension): 7 mL of NaHCO3 was added to the sterilised water during test water reconstitution (instead of 1 mL) to obtain a final concentration of 350 mg.L-1, according to the study plan.
OTHER TEST CONDITIONS
- Sterile test conditions: Yes
- Photoperiod: Continuous illumination
- Light intensity and quality: Continuous illumination with a light intensity of 4,440-8,880 lux and did not vary more than ± 15% from the average light intensity over the incubation area.
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Cell numbers were counted daily by microscope using a counting chamber.
- Other:
pH: At the start and the end of the test in one vessel per concentration and the control (same vessel at t=0h and t=72h).
Temperature of Medium: Measured continuously in the growth chamber, over the study period.
Light Intensity: Light intensity was measured once (t=0 h) during the test at 5 positions distributed over the experimental area at the surface of the test media.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.78
- Results used to determine the conditions for the definitive study: Based on the results of a range-finding test, test solutions used in the definitive test were prepared to obtain the following loading rates: 10.0; 17.7; 31.6; 56.2 and 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 54.883 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% conf. limits: 51.375 - 57.932 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 39.764 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% conf. limits: 31.099 - 44.620 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EL20
- Effect conc.:
- 66.537 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% conf. limits: 63.595 - 69.110 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EL20
- Effect conc.:
- 45.605 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% conf. limits: 38.657 - 49.559 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 96.174 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% conf. limits: 93.787 - 98.785 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 59.277 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% conf. limits: 55.773 - 63.667 mg/L
- Results with reference substance (positive control):
- On February 14, 2017 (most recent test), the 72h-EC50 was 1.438 mg.L-1 for the parameter growth rate. Hence, the sensitivity of this batch of Pseudokirchneriella subcapitata was consistent with the level proposed by the ISO 8692 (expected 72h-EC50: 0.92 mg.L-1 to 1.46 mg.L-1).
- Reported statistics and error estimates:
- The evaluation of the effects was based on the nominal WAFs concentrations (nominal loading values). The software ToxRat® Professional was used for the determination of the effective concentrations.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the experimental conditions and based on nominal concentrations, the 72-hour EL50 for the parameters growth rate and yield were determined to be 96.174 mg.L-1 and 59.277 mg.L-1, respectively. The 72-hour EL10 for growth rate was 54.883 mg L-1 and for yield was 39.764 mg.L-1.
- Executive summary:
A study was performed according to OECD Guideline 201 added by WAF protocol according to OECD 23 on complex substances, to assess the test item, LAVANDIN CONCRETE for its ability to generate toxic effects in Pseudokirchneriella subcapitata, under static conditions.
Following a preliminary range-finding test, algal cells were exposed toWater Accommodated Fractions (WAFs)of the test item over a range of nominal loading values of 10.0, 17.7, 31.6, 56.2 and 100.0 mg.L-1and to a control. The inhibition of growth in relation to control cultures was determined over a test period of 72 hours, and thus over several algal generations.Concentration of dissolved organic material in the control and the WAFs was checked by TOC analysis at the start and the end of the test.
Overall, the analytical results of this test showed that WAFs concentrations were stable throughout the experiment.
Under the experimental conditions and based on nominal concentrations, the 72-hour EL50 for the parameters growth rate and yield were determined to be 96.174 mg.L-1 and 59.277 mg.L-1, respectively. The 72-hour EL10 for growth rate was 54.883 mg L-1 and for yield was 39.764 mg.L-1.
Reference
Table 6.1.5/1: pH-values during the final test
|
Nominal concentration(mg test item.L-1)* |
|||||
Control |
10.0 |
17.7 |
31.6 |
56.2 |
100.0 |
|
Start t=0h |
8.37 |
8.32 |
8.30 |
8.44 |
8.43 |
8.32 |
End t=72h |
10.15 |
9.96 |
10.30 |
10.42 |
9.96 |
8.88 |
* WAF prepared at the given loading rate.
Table 6.1.5/2: Algal cell densities during the final test (expressed as density of algal cells/mL x 104)
|
Replicate |
Nominal concentration (mg test item.L-1)*
|
|||||
Control |
10.0 |
17.7 |
31.6 |
56.2 |
100.0 |
||
t=24h |
1 |
7.2 |
4.8 |
6.0 |
5.2 |
4.4 |
2.4 |
2 |
8.4 |
5.2 |
5.2 |
6.0 |
3.6 |
2.4 |
|
3 |
6.4 |
6.4 |
4.4 |
7.6 |
4.4 |
3.2 |
|
4 |
6.0 |
|
|
|
|
|
|
5 |
6.0 |
|
|
|
|
|
|
6 |
5.6 |
|
|
|
|
|
|
Mean |
6.6 |
5.5 |
5.2 |
6.3 |
4.1 |
2.7 |
|
Std. Dev. |
1.04 |
0.83 |
0.80 |
1.22 |
0.46 |
0.46 |
|
t=48h |
1 |
29.6 |
24.4 |
27.6 |
25.2 |
24.0 |
4.0 |
2 |
27.2 |
24.8 |
31.6 |
25.2 |
18.0 |
3.6 |
|
3 |
28.0 |
27.6 |
23.6 |
26.0 |
20.0 |
4.0 |
|
4 |
24.4 |
|
|
|
|
|
|
5 |
30.0 |
|
|
|
|
|
|
6 |
30.8 |
|
|
|
|
|
|
Mean |
28.3 |
25.6 |
27.6 |
25.5 |
20.7 |
3.9 |
|
Std. Dev. |
2.34 |
1.74 |
4.00 |
0.46 |
3.06 |
0.23 |
|
t=72h |
1 |
84.0 |
81.6 |
100.0 |
94.0 |
56.0 |
6.8 |
2 |
90.0 |
83.2 |
98.0 |
96.0 |
46.0 |
5.2 |
|
3 |
94.8 |
94.0 |
72.8 |
96.0 |
48.0 |
4.8 |
|
4 |
81.6 |
|
|
|
|
|
|
5 |
84.0 |
|
|
|
|
|
|
6 |
96.8 |
|
|
|
|
|
|
Mean |
88.5 |
86.3 |
90.3 |
95.3 |
50.0 |
5.6 |
|
Std. Dev. |
6.31 |
6.74 |
15.16 |
1.15 |
5.29 |
1.06 |
* WAF prepared at the given loading rate.
At test start 5000 algal cells/mL were incubated; 6 replicates of the controls and 3 replicates of each test loading rate.
Std. Dev.: standard deviation.
Validity criteria of the study
- Cell density in Controls: 177-fold increase within 72 hours. The specific growth rate was determined at 1.73 day-1, so greater than 0.92 day-1.
- Coefficient of Variation:
1. The mean coefficient of variation for section-by-section specific growth rates in the control cultures was of 9%.
2. The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures was of 1.4%.
Thus the validity criteria were respected in this study.
Analytical results
Concentration of dissolved organic material in the control and the WAFs was checked by TOC analysis at the start and at the end of the test.
Due to the complex nature of the substance, it was difficult to well solubilize the substance in the water medium(heating, sonication, mixingwithout headspace …). Even if the TOCanalysisindicated that organic compounds were found at low value in the loading rates, analytical resultsshowed thatWAFs concentrations were overall stablebetween the start and the end of the exposure period. Besides, it would be difficult to determine the specific cause of the high TOC content at 10 mg.L-1(higher than expected in comparison with other loading rates). However, this was considered not to affect the results and the integrity of the study regardingthe biological results.
It should be noted that aWAF is by definition a complex mixture for which the individual concentration of each constituent differs due to its properties (e.g. solubility, adsorption, volatilisation, bioaccumulation…). Due to these differences, interactions between certain constituents of the mixture may occur and affect the behaviour of a given constituent which consequently would not react in the same way that if it was alone in the mixture.Therefore, and since the test item was a UVCB substance, the results were based on thenominaltest loading rates.
Table 6.1.5/3: Concentrations of the test item in test water - Results of the determination of TOC analysis (mg.L-1) - Final test.
Nominal concentration* (mg test item.L-1) |
Start (t=0h) |
End (t=72h) |
Control |
0.61 |
0.92 |
10.0 |
6.17 |
5.51 |
17.7 |
1.44 |
1.61 |
31.6 |
4.41 |
4.60 |
56.2 |
17.3 |
19.4 |
100.0 |
25.5 |
23.8 |
* WAF prepared at the given loading rate.
Physico-chemical parameter values throughout the test
Although the pH level in the control varied more than 1.5 units at the end of the test (1.78 units of difference) this was not considered to have affected the integrity of the study. The cause of the pH increases in the controls and test concentrations was certainly due to the substantial algal growth in conjunction with closed conditions used in the test, despite the addition of moresodium bicarbonate.
The mean light intensity was of 5963 lux (range: 5463-6460 lux), which remainedwithin the rangesprescribed by the study plan (range: 4440-8880 lux and shall not vary more than ± 15% from the average light intensity over the incubation area).
The temperature in the incubator was situated between 22.8 and 23.0°C throughout the test (average value: 22.9°C), and complied with the requirements as laid down in the study plan (23°C ± 2°C, constant within 2°C).
Description of key information
Under the experimental conditions and based on nominal concentrations, the 72-hour EL50 for the parameters growth rate and yield were determined to be 96.174 mg.L-1 and 59.277 mg.L-1, respectively. The 72-hour EL10 for growth rate was 54.883 mg L-1 and for yield was 39.764 mg.L-1.
Based on the result of this study, the substance LAVANDIN CONCRETE would not be acutely toxic to aquatic organisms in accordance with the classification of the CLP.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 96.174 mg/L
- EC10 or NOEC for freshwater algae:
- 54.883 mg/L
Additional information
A reliable study was available to assess the test item, LAVANDIN CONCRETE for its ability to generate toxic effects in Pseudokirchneriella subcapitata, under static conditions.
Following a preliminary range-finding test, algal cells were exposed to Water Accommodated Fractions (WAFs) of the test itemover a range of nominal loading values of 10.0, 17.7, 31.6, 56.2 and 100.0mg.L-1and to a control. The inhibition of growth in relation to control cultures was determined over a test period of 72 hours, and thus over several algal generations.Concentration of dissolved organic material in the control and the WAFs was checked by TOC analysisat the start and the end of the test.
Overall, the analytical results of this test showed that WAFs concentrations were stable throughout the experiment.
This toxicity study was considered acceptable for that endpoint.
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