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EC number: 205-771-9 | CAS number: 150-78-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No GLP conditions
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella mutagenicity test results for 250 chemicals
- Author:
- HAWORTH S, LAWLOR T, MORTELMANS K, SPECK W, ZEIGER E
- Year:
- 1 983
- Bibliographic source:
- Environ. Mutagenesis Supplement vol. 5, sup. 1, p : 3-142
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: NTP protocols
- Deviations:
- no
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,4-dimethoxybenzene
- EC Number:
- 205-771-9
- EC Name:
- 1,4-dimethoxybenzene
- Cas Number:
- 150-78-7
- Molecular formula:
- C8H10O2
- IUPAC Name:
- 1,4-dimethoxybenzene
- Details on test material:
- Test material identity: From Aldrich
lot/batch No: MC 022287.
Analytical purity: 98%
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Induced S9 mix with Aroclor 1254, from rat or hamster liver cells.
- Test concentrations with justification for top dose:
- 0; 10; 33; 100; 333; 666; 750; 900; 1000; 2000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: solubility of the test material
Controls
- Untreated negative controls:
- yes
- Remarks:
- choline chloride, glycerol, glycine, mannitol and sodium phosphate.
- Negative solvent / vehicle controls:
- yes
- Remarks:
- culture medium
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene on all strains with rat (1.5 µg/plate) and hamster (0.75 µg/plate) S9; 4-Nitro-o-phenylenediamine on TA98 without S9 (12µg/plate); sodium azide on TA100 and TA1535 without S9 (2.5 µg/plate); 9-aminoacridine on TA1537 (80 µg/plate)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation with S9 mix or buffer
DURATION
- pre-incubation period: 20 min at 37°C
- Exposure duration of incubation: 48 h at 37°C - Evaluation criteria:
- Although procedures for the statistical analysis of Salmonella plate test data have been developed [Margolin et al, 1981], they were not incorporated
into the initial data evaluations. The data were evaluated in an ad hoc manner by each testing laboratory and by NTP personnel. Prior to statistical
analysis no formal rules were used; however, a positive response was indicated by a reproducible, dose-related increase, whether it be twofold over
background or not. The matrix of test strains and activation systems used allowed the investigators to detect trends or patterns that might not be
as evident if only one strain and activation system were examined. In addition to the standard "positive" and "negative" categories, there is also
"questionable" (or "inconclusive"). This applied to low-level responses that were not reproducible within the laboratory or to results that showed a
definite trend but with which the investigator did not feel comfortable in making a " +" or " -" decision. It also included tests in which an elevated
revertant colony yield occurred at only a single dose level. After a decision on the mutagenicity of a sample was made, a request to decode the
sample was sent to the repository, and the code was broken. - Statistics:
- The data were subsequently evaluated using an analysis based on the models presented by Margolin et al [1981]. As a result of these statistical
analyses, a number of calls were changed from the original "negative" to "equivocal." The statistical analysis did not result in any "positive" or
"equivocal" calls being called "negative".
Because the criteria for "positive" or "questionable" decisions have evolved during the course of the study and because the recent use of statistical
analysis, some of the results differ from the initial evaluations published in the NTP Technical Bulletins (1980 to 1983).
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- yes, slight to toxic for all strains at high concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Strain: TA1535
Dose |
No Activation |
No Activation |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||||
Dose units |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
23 |
2 |
13 |
1.8 |
26 |
1.2 |
11 |
2.6 |
9 |
1.8 |
7 |
1.2 |
11 |
0.9 |
11 |
0.9 |
10 |
|
|
20 |
1.7 |
|
|
|
|
|
|
6 |
0.9 |
|
|
6 |
0.6 |
33 |
33 |
1.7 |
21 |
2.6 |
21 |
4.2 |
17 |
1 |
13 |
1.3 |
7 |
2 |
10 |
1 |
9 |
2.5 |
100 |
27 |
2 |
23 |
0.7 |
18 |
3.8 |
10 |
0.7 |
11 |
0.6 |
7 |
1.2 |
14 |
3.5 |
9 |
2.7 |
333 |
24 |
6.4 |
41 |
1.5 |
21 |
1.8 |
15 |
1.8 |
9 |
2 |
8 |
1.5 |
10 |
1.8 |
6 |
1.2 |
666 |
|
|
106 |
3 |
23s |
0.6 |
14s |
1 |
|
|
7 |
1.2 |
|
|
7 |
1.2 |
750 |
|
|
|
|
18s |
1.5 |
13s |
1.9 |
|
|
|
|
|
|
|
|
900 |
|
|
|
|
14s |
5.8 |
8s |
4.1 |
|
|
|
|
|
|
|
|
1000 |
T |
|
|
|
|
|
|
11s |
0.5 |
|
|
5s |
1 |
|
|
|
2000 |
T |
|
|
|
|
|
|
T |
|
|
T |
|
|
|||
Positive Control |
1408 |
20.8 |
1446 |
11.7 |
2002 |
67.1 |
1413 |
91.8 |
148 |
6.8 |
85 |
5.9 |
77 |
1 |
53 |
8. |
Strain: TA100
Dose |
No Activation |
No Activation |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||||
Dose units |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
97 |
2.1 |
82 |
5 |
103 |
11.9 |
108 |
6.7 |
85 |
6.9 |
82 |
6.1 |
92 |
13.2 |
81 |
6.2 |
10 |
|
|
88 |
12.9 |
|
|
|
|
|
|
83 |
8.6 |
|
|
70 |
8.1 |
33 |
108 |
9.8 |
91 |
4.3 |
99 |
4.5 |
103 |
15.9 |
91 |
3.8 |
75 |
2.2 |
98 |
6.2 |
74 |
2.9 |
100 |
124 |
2 |
99 |
9.5 |
105 |
8.5 |
107 |
7.6 |
84 |
6.5 |
106 |
8.4 |
98 |
4.3 |
82 |
8.4 |
333 |
109 |
5.4 |
141 |
2.3 |
96 |
4.7 |
94 |
8.7 |
92 |
5.2 |
83 |
0.9 |
99 |
9 |
81 |
8.9 |
666 |
|
|
246 |
17.2 |
101 |
3.8 |
114s |
12.7 |
|
|
75 |
5 |
|
|
84 |
3.5 |
750 |
|
|
|
|
105s |
8.1 |
116s |
10.1 |
|
|
|
|
|
|
|
|
900 |
|
|
|
|
74s |
33.5 |
62s |
27 |
|
|
|
|
|
|
|
|
1000 |
T |
|
|
|
|
|
|
69s |
9.5 |
|
|
T |
|
|
||
2000 |
T |
|
|
|
|
|
|
T |
|
|
T |
|
|
|||
Positive Control |
1919 |
39.3 |
2152 |
99.5 |
2173 |
58.7 |
1590 |
94.7 |
1634 |
59.6 |
861 |
16.2 |
1303 |
116.4 |
457 |
35.9 |
Strain: TA98
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
Dose units |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
19 |
2.5 |
18 |
5.1 |
29 |
3.3 |
23 |
0.9 |
20 |
3.8 |
22 |
2.9 |
10 |
|
|
16 |
3.6 |
|
|
16 |
1.7 |
|
|
21 |
2.4 |
33 |
14 |
0.9 |
17 |
3.8 |
27 |
2.3 |
22 |
3.2 |
22 |
1.2 |
24 |
0.7 |
100 |
17 |
2.3 |
20 |
4.1 |
29 |
2.4 |
22 |
3 |
19 |
4.1 |
22 |
3.1 |
333 |
16 |
1.5 |
15 |
3.3 |
23 |
0.9 |
15 |
1.9 |
33 |
1.7 |
21 |
1.2 |
666 |
|
|
14 |
3.3 |
|
|
21 |
1.5 |
|
|
20 |
1.2 |
1000 |
T |
|
|
27s |
6.5 |
|
|
T |
|
|
||
2000 |
T |
|
|
T |
|
|
T |
|
|
|||
Positive Control |
1174 |
17.5 |
1352 |
55.2 |
983 |
53.9 |
808 |
31.9 |
529 |
73.4 |
532 |
18.2 |
Strain: TA1537
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
Dose units |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
8 |
1.9 |
3 |
0.6 |
10 |
1.7 |
5 |
0.7 |
10 |
1 |
6 |
2.6 |
10 |
|
|
5 |
1.2 |
|
|
6 |
0.3 |
|
|
5 |
0.6 |
33 |
6 |
0.7 |
4 |
1.2 |
9 |
0.3 |
4 |
1.2 |
7 |
1.5 |
3 |
0.3 |
100 |
6 |
0.9 |
3 |
0.9 |
7 |
0.7 |
3 |
0.7 |
10 |
3.7 |
4 |
0.6 |
333 |
8 |
1.3 |
4 |
1.2 |
10 |
2.3 |
5 |
0.7 |
9 |
0.9 |
8 |
2.5 |
666 |
|
|
5 |
0.7 |
|
|
5 |
1.2 |
|
|
7 |
3.7 |
1000 |
T |
|
|
8s |
1 |
|
|
9s |
2.6 |
|
|
|
2000 |
T |
|
|
T |
|
|
T |
|
|
|||
Positive Control |
383 |
200.3 |
518 |
52.7 |
206 |
29.5 |
105 |
14.5 |
73 |
4.7 |
58 |
3.5 |
Applicant's summary and conclusion
- Conclusions:
Negative with and without metabolic acivation.- Executive summary:
In a reverse gene mutation assay in bacteria (Haworth, 1983), strains TA98, TA100, TA1535 and TA1537 of S. typhimurium were exposed to paradimethoxybenzene at concentrations of 0 to 2000 µg/plate in the presence and absence of mammalian metabolic activation (rat or hamster liver cells).
Paradimethoxybenzene was tested up to cytotoxic concentrations.
The positive controls induced the appropriate responses in the corresponding strains. There was no evidence of induced mutant colonies over background.
This study is classified as acceptable. This study satisfies the requirement for Test Guideline OECD 471 for in vitro mutagenicity bacterial reverse gene mutation data.
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