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EC number: 616-628-9 | CAS number: 78543-37-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 1996-01-21
Test material
- Reference substance name:
- potassium dibenzyl phosphate
- EC Number:
- 616-628-9
- Cas Number:
- 78543-37-0
- Molecular formula:
- C14-H14-O4-P.K
- IUPAC Name:
- potassium dibenzyl phosphate
- Test material form:
- solid
- Details on test material:
- Batch 97/8
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test material was prepared by a direct dispersion in water.
An amount of 100mg was dispersed in reconstituted water and the volume adjust to 1 litre to give the 100 mg/L test concentration.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Justification for species other than prescribed by test guideline: common species
- Source: Daphnia magnawere maintained in a laboratory culture originating from a strain supplied by the Institut National de Recherche Chilique Appliquée (IRCHA), France.
- Feeding during test: No
ACCLIMATION
- No acclimatation: internal culture
- Type and amount of food: feed daily with a suspension of mixed algae (Predominantly Chlorella spp)
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Adult Daphnia were maintained in polypropylene vessels containing approximately 2 litres of reconstituted water in a temperature controlled room at 21 °C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle. Each culture was fed daily with a suspension of mixed algae (predominantly Chlore/la spp.).
Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated 24 hours prior to the initiation of the test; the young daphnids produced overnight were then removed for testing. The diet and diluent water are considered not to contain any contaminant that would affect the integrity or outcome of the study.
Study design
- Test type:
- static
- Water media type:
- other: Reconstituted water
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- approximately 270 mg/L CaCO3
- Test temperature:
- 21 °C
- pH:
- 7.8 to 7.9
- Dissolved oxygen:
- 7.6 to 8.1 mg O2/L
- Salinity:
- Not applicable: Fresh water
- Conductivity:
- < 5 µS
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass jars
- Material, size, headspace, fill volume: 200 mL
- Aeration: Covered to prevent evaporation
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
Reconstituted water
- CaCl2.2H2O: 11.76 g/L
- MgSO4.7H2O: 4.93 g/L
- NaHCO3: 2.59 g/L
- HCl: 0.23 g/L
OTHER TEST CONDITIONS
- Adjustment of pH: if neccessary
- Photoperiod: 16h light and 8h darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mobility
RANGE-FINDING STUDY
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: No immobilisation duriong the range finding test at 100 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Any other information on results incl. tables
Cumulative immobilization data in the range-finding study:
Nominal concentration (mg/L) |
Cumulative immobilized Daphnia Initial Population: 10 per replicate |
|
Control |
0 |
0 |
0.10 |
0 |
0 |
1.0 |
0 |
0 |
10 |
0 |
0 |
100 |
0 |
0 |
Cumulative immobilization data in the definitive study:
Nominal concentration (mg/L) |
Cumulative immobilized Daphnia Initial Population: 10 per replicate |
||||||
24 hours |
48 hours |
||||||
Number per replicate |
Total |
% |
Number per replicate |
Total |
% |
||
Control |
R1 |
0 |
0 |
0 |
0 |
0 |
0 |
R2 |
0 |
0 |
|||||
100 |
R1 |
0 |
0 |
0 |
0 |
0 |
0 |
R2 |
0 |
0 |
|||||
R3 |
0 |
0 |
|||||
R4 |
0 |
0 |
R1 – R4: Replicates 1 - 4
Physico-chemical measurments:
Nominal concentration (mg/L) |
0 Hours |
24 hours |
48 hours |
|||||
pH |
mg O2/L |
T° (°C) |
T° (°C) |
pH |
mg O2/L |
T° (°C) |
||
Control |
R1 |
7.9 |
8.1 |
21 |
21 |
7.8 |
7.6 |
21 |
R2 |
7.9 |
8.1 |
21 |
21 |
7.8 |
7.7 |
21 |
|
100 |
R1 |
7.9 |
8.1 |
21 |
21 |
7.8 |
7.7 |
21 |
R2 |
7.9 |
8.1 |
21 |
21 |
7.8 |
7.7 |
21 |
|
R3 |
7.9 |
8.1 |
21 |
21 |
7.9 |
7.7 |
21 |
|
R4 |
7.9 |
8.1 |
21 |
21 |
7.8 |
7.6 |
21 |
R1 – R4: Replicates 1 - 4
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 of the Dibenzyl Potassium Phosphate on Daphnia Magna has been determinated to be greater than 100 mg/L
- Executive summary:
The acute toxicty on aquatic invertebrates of Dibenzyl Potassium Phosphate has been determinated according to OECD 202 test guideline in compliance with GLP.
The 48h-EC50 was determinated to be greater than 100 mg/L.
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