Registration Dossier
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EC number: 267-636-0 | CAS number: 67905-17-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from experimental study report
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- - Pre-treatment of test chemical: The in-house water solubility for the test chemical is 0.22 mg/L. Therefore, the test chemical will be prepared by dissolving 1000 mg of test chemical in 1000 mL of M7 media with 48 hours stirring to get the final concentration of 1000 mg/L. This stock solution will be filtered by using whatman filter paper no. 42, which will then analytically determine. The final solubility value obtained in media was used to prepare the remaining test concentrations from the above stock solution.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Test chemical was weighed and about 1000mg in 1000 ml of test chemical kept for stirring for 48 hours, which was then filtered and the concentration was anlytically determined, and which was obtained to be 0.1 mg/L
- Controls: Test media M7 was directly used as the control.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehilce was used during study
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): no applicable - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Age at study initiation (mean and range, SD): < 24 hr old
- Length at study initiation (length definition, mean, range and SD): 0.45 cm
- Source: Eggs of Daphnia magna were obtained from Denmark Technical University and maintained at Unique Ecotox Research Laboratory, Nagpur
ACCLIMATION
- Type and amount of food: Living cells of Selenestrum capricornutum
- Feeding frequency: Feeding of the Daphnia with living cells of Selenestrum capricornutum was done thrice a week.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
- Hardness:
- 140 mg/l as CaCO3
- Test temperature:
- 18-22°C
- pH:
- 7.4-7.9
- Dissolved oxygen:
- 7.4-8.1mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- 0.1 mg/l ( nominal concentration)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beaker
- Material, size, headspace, fill volume: 50 ml
- Aeration: Test vessel were not aerated during the study.
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
TEST MEDIUM / WATER PARAMETERS: M7 medium
OTHER TEST CONDITIONS
- Photoperiod: 16:8 light:dark conditions
- Light intensity: Not exceeding 1000-1500 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The evaluation of the NOEC was determined based on the number of offspring’s produced per living parent Daphnia.
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Behavioural abnormalities:
no behavioural abnormalities were observed
- Observations on body length and weight: no significant difference was observed in the body length was observed in both parents and offsprings
- Other biological observations: first brood with visible eggs in parents was observed on day 11
- Mortality of control: no moratality was observed in control parents and offsprings
- Other adverse effects control: no adverse effects were observed
- Immobilisation of control: no immobilisation - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- The statistical comparision was done by student T test method using Sys stat software
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the effect on reproduction of the test daphnids, the 21 d NOEC value was determined to be 0.1 mg/l.
- Executive summary:
A chronic study was conducted for assesing the effect of test chemical. The study was performed in accordance with the principles of the OECD Guideline 211 (Daphnia magna Reproduction Test). Daphnia magna of length 0.45 cm was used as a test organism. Eggs of Daphnia magna were obtained from Denmark Technical University. Test daphnids were fed with living cells of Selenestrum capricornutum and it was done thrice a week. The in-house water solubility for the test chemical is 0.22 mg/L. Therefore, the test chemical will be prepared by dissolving 1000 mg of test chemical in 1000 mL of M7 media with 48 hours stirring to get the final concentration of 1000 mg/L. This stock solution will be filtered by using whatman filter paper no. 42, which will then analytically determine. The final solubility value obtained in media was used to prepare the remaining test concentrations from the above stock solution. The analytical determinations were performed by UV-VIS spectrophotometer. The pre-treated stock solution was then diluted with media in order to get the required test solutions ranging from concentrations, 0.1, 0.25, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, and 7.0 mg/L. The absorbance of resulting solution was measured using UV-VIS spectrophotometer against corresponding blank at lambda max (λmax). Standard curve was plotted against concentration verses absorbance and the maximum solubility was determined from the below standard curve. Analytical assessments were performed for selected test concentration at 1st, 2nd and 3rd week. The concentration of the test chemical being tested has been satisfactorily maintained within ± 20 % of the nominal concentration throughout the test. Therefore, the analysis of the results was based on nominal concentration. Test chemical conc. used in the defiinite study was 0.1 mg/l. Thus, limit test was performed at 0.1 mg/l conc. Test daphnids were exposed to test chemical conc. in a glass beaker for an exposure period of 21 days. Vessels were not aerated during the study. M7 medium was used as a test medium. Test conditions involve a temperature range of 18-22°C, 16:8 light:dark conditions and light intensity not exceeding 1000-1500 lux, respectively. The evaluation of the NOEC was determined based on the number of offspring’s produced per living parent Daphnia. Defined concentrations of the test chemical led to a certain percentage reduction of the parthenogenetic reproduction rate at the end of the 21 day study period. The living offspring was counted daily along with the renewal of the test medium. However, test vessels were inspected daily for the occurrence of juveniles and marked accordingly. On the basis of the effect on reproduction of the test daphnids, the 21 d NOEC value was determined to be 0.1 mg/l. Thus based on the outcomes chemical could be classified into aquatic chronic category 1 as per CLP classification criteria.
Reference
Defined concentrations of the test chemical led to a certain percentage reduction of the parthenogenetic reproduction rate at the end of the 21 day study period. The living offspring was counted daily along with the renewal of the test medium. However, test vessels were inspected daily for the occurrence of juveniles and marked accordingly.
Control
Day |
0 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
|
Medium renewal |
- |
- |
Y |
- |
Y |
- |
Y |
- |
Y |
- |
- |
Y |
- |
Y |
- |
Y |
- |
- |
Y |
- |
Y |
- |
|
pH (new) |
7.4 |
- |
7.9 |
- |
7.3 |
- |
7.8 |
- |
7.8 |
- |
- |
7.4 |
- |
7.3 |
- |
7.5 |
- |
- |
7.4 |
- |
7.5 |
- |
|
O2 (mg/l) (new) |
7.5 |
- |
8.0 |
- |
7.8 |
- |
7.9 |
- |
7.8 |
- |
- |
7.8 |
- |
7.9 |
- |
7.6 |
- |
- |
7.9 |
- |
7.7 |
- |
|
Temp (°C) (new) |
18 |
- |
21 |
- |
20.7 |
- |
21.0 |
- |
21.6 |
- |
- |
21.8 |
- |
21.3 |
- |
21.5 |
- |
- |
21.9 |
- |
21.5 |
- |
|
Food provided |
Y |
- |
Y |
- |
Y |
- |
Y |
- |
Y |
- |
- |
Y |
- |
Y |
- |
Y |
- |
- |
Y |
- |
Y |
- |
|
Replicates |
Number of living offspring per replicate |
Total no. of live offsprings per vessel |
|||||||||||||||||||||
V1 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
15 |
- |
- |
15 |
- |
11 |
16 |
- |
- |
57 |
V2 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
10 |
- |
- |
26 |
- |
- |
9 |
- |
- |
- |
45 |
V3 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
10 |
- |
- |
- |
10 |
- |
11 |
26 |
57 |
V4 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
#25 |
- |
15 |
16 |
4 |
- |
- |
7 |
- |
13 |
- |
80 |
V5 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
7 |
- |
20 |
- |
- |
21 |
- |
12 |
7 |
- |
- |
67 |
V6 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
15 |
- |
25 |
- |
- |
- |
- |
16 |
- |
14 |
- |
70 |
V7 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
15 |
- |
6 |
- |
19 |
- |
21 |
- |
21 |
- |
82 |
V8 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
11 |
- |
- |
15 |
- |
12 |
15 |
- |
- |
53 |
V9 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
18 |
- |
- |
- |
13 |
- |
7 |
11 |
- |
- |
49 |
V10 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
10 |
- |
5 |
- |
- |
- |
21 |
- |
- |
16 |
52 |
Cumulative Reproductivity |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
612 |
No. of Days |
0 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
|
V1 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V2 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V3 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V4 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V5 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V6 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V7 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V8 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V9 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V10 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Nominal Concentration: 0.10 mg/L
Day |
0 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
|
Medium renewal |
- |
- |
Y |
- |
Y |
- |
Y |
- |
Y |
- |
- |
Y |
- |
Y |
- |
Y |
- |
- |
Y |
- |
Y |
- |
|
pH (new) |
7.5 |
- |
7.9 |
- |
7.5 |
- |
7.9 |
- |
7.8 |
- |
- |
7.5 |
- |
7.4 |
- |
7.5 |
- |
- |
7.4 |
- |
7.8 |
- |
|
O2 (mg/l) (new) |
8.1 |
- |
8.0 |
- |
7.9 |
- |
8.0 |
- |
7.4 |
- |
- |
7.9 |
- |
7.9 |
- |
8.0 |
- |
- |
7.7 |
- |
7.4 |
- |
|
Temp (°C) (new) |
18 |
- |
21 |
- |
21.2 |
- |
21.0 |
- |
21.8 |
- |
- |
21.8 |
- |
21.0 |
- |
21.5 |
- |
- |
21.9 |
- |
21.7 |
- |
|
Food provided |
Y |
- |
Y |
- |
Y |
- |
Y |
- |
Y |
- |
- |
Y |
- |
Y |
- |
Y |
- |
- |
Y |
- |
Y |
- |
|
Replicates |
Number of living offspring per replicate |
Total no. of live offspring’s per vessel |
|||||||||||||||||||||
V1 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
#5 |
- |
1 |
10 |
- |
- |
12 |
10 |
2 |
15 |
- |
55 |
V2 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
5 |
1 |
12 |
5 |
- |
- |
12 |
5 |
5 |
- |
45 |
V3 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
11 |
- |
- |
8 |
- |
9 |
- |
15 |
- |
- |
- |
43 |
V4 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
1 |
- |
- |
6 |
12 |
- |
- |
5 |
- |
14 |
16 |
54 |
V5 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
4 |
- |
13 |
- |
2 |
- |
- |
2 |
17 |
- |
10 |
48 |
V6 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
10 |
- |
- |
25 |
- |
- |
- |
7 |
- |
13 |
12 |
67 |
V7 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
10 |
- |
15 |
- |
- |
- |
21 |
- |
- |
46 |
V8 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
8 |
- |
2 |
- |
- |
9 |
25 |
2 |
15 |
61 |
V9 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
21 |
10 |
- |
- |
4 |
- |
- |
- |
14 |
- |
- |
49 |
V10 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
3 |
- |
14 |
- |
- |
- |
10 |
- |
21 |
48 |
Cumulative Reproductivity |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
516 |
No. of Days |
0 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
|
V1 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V2 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V3 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V4 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V5 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V6 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V7 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V8 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V9 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
V10 |
Dead P |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Dead O |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Description of key information
Long term toxicity to aquatic invertebrate:
A chronic study was conducted for assesing the effect of test chemical. The study was performed in accordance with the principles of the OECD Guideline 211 (Daphnia magna Reproduction Test). Daphnia magna of length 0.45 cm was used as a test organism. Eggs of Daphnia magna were obtained from Denmark Technical University. Test daphnids were fed with living cells of Selenestrum capricornutum and it was done thrice a week. DMSO or acetone was used as a vehicle. The in-house water solubility for the test chemical is 0.22 mg/L. Therefore, the test chemical will be prepared by dissolving 1000 mg of test chemical in 1000 mL of M7 media with 48 hours stirring to get the final concentration of 1000 mg/L. This stock solution will be filtered by using whatman filter paper no. 42, which will then analytically determine. The final solubility value obtained in media was used to prepare the remaining test concentrations from the above stock solution. The analytical determinations were performed by UV-VIS spectrophotometer. The pre-treated stock solution was then diluted with media in order to get the required test solutions ranging from concentrations, 0.1, 0.25, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, and 7.0 mg/L. The absorbance of resulting solution was measured using UV-VIS spectrophotometer against corresponding blank at lambda max (λmax). Standard curve was plotted against concentration verses absorbance and the maximum solubility was determined from the below standard curve. Analytical assessments were performed for selected test concentration at 1st, 2nd and 3rd week. The concentration of the test chemical being tested has been satisfactorily maintained within ± 20 % of the nominal concentration throughout the test. Therefore, the analysis of the results was based on nominal concentration. Test chemical conc. used in the defiinite study was 0.1 mg/l. Thus, limit test was performed at 0.1 mg/l conc. Test daphnids were exposed to test chemical conc. in a glass beaker for an exposure period of 21 days. Vessels were not aerated during the study. M7 medium was used as a test medium. Test conditions involve a temperature range of 18-22°C, 16:8 light:dark conditions and light intensity not exceeding 1000-1500 lux, respectively. The evaluation of the NOEC was determined based on the number of offspring’s produced per living parent Daphnia. Defined concentrations of the test chemical led to a certain percentage reduction of the parthenogenetic reproduction rate at the end of the 21 day study period. The living offspring was counted daily along with the renewal of the test medium. However, test vessels were inspected daily for the occurrence of juveniles and marked accordingly. On the basis of the effect on reproduction of the test daphnids, the 21 d NOEC value was determined to be 0.1 mg/l. Thus based on the outcomes chemical could be classified into aquatic chronic category 1 as per CLP classification criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.1 mg/L
Additional information
Long term toxicity to aquatic invertebrate:
A chronic study was conducted for assesing the effect of test chemical. The study was performed in accordance with the principles of the OECD Guideline 211 (Daphnia magna Reproduction Test). Daphnia magna of length 0.45 cm was used as a test organism. Eggs of Daphnia magna were obtained from Denmark Technical University. Test daphnids were fed with living cells of Selenestrum capricornutum and it was done thrice a week. DMSO or acetone was used as a vehicle. The in-house water solubility for the test chemical is 0.22 mg/L. Therefore, the test chemical will be prepared by dissolving 1000 mg of test chemical in 1000 mL of M7 media with 48 hours stirring to get the final concentration of 1000 mg/L. This stock solution will be filtered by using whatman filter paper no. 42, which will then analytically determine. The final solubility value obtained in media was used to prepare the remaining test concentrations from the above stock solution. The analytical determinations were performed by UV-VIS spectrophotometer. The pre-treated stock solution was then diluted with media in order to get the required test solutions ranging from concentrations, 0.1, 0.25, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, and 7.0 mg/L. The absorbance of resulting solution was measured using UV-VIS spectrophotometer against corresponding blank at lambda max (λmax). Standard curve was plotted against concentration verses absorbance and the maximum solubility was determined from the below standard curve. Analytical assessments were performed for selected test concentration at 1st, 2nd and 3rd week. The concentration of the test chemical being tested has been satisfactorily maintained within ± 20 % of the nominal concentration throughout the test. Therefore, the analysis of the results was based on nominal concentration. Test chemical conc. used in the defiinite study was 0.1 mg/l. Thus, limit test was performed at 0.1 mg/l conc. Test daphnids were exposed to test chemical conc. in a glass beaker for an exposure period of 21 days. Vessels were not aerated during the study. M7 medium was used as a test medium. Test conditions involve a temperature range of 18-22°C, 16:8 light:dark conditions and light intensity not exceeding 1000-1500 lux, respectively. The evaluation of the NOEC was determined based on the number of offspring’s produced per living parent Daphnia. Defined concentrations of the test chemical led to a certain percentage reduction of the parthenogenetic reproduction rate at the end of the 21 day study period. The living offspring was counted daily along with the renewal of the test medium. However, test vessels were inspected daily for the occurrence of juveniles and marked accordingly. On the basis of the effect on reproduction of the test daphnids, the 21 d NOEC value was determined to be 0.1 mg/l. Thus based on the outcomes chemical could be classified into aquatic chronic category 1 as per CLP classification criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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