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EC number: 264-859-5 | CAS number: 64381-99-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from secondary source
Data source
Reference
- Reference Type:
- other: Secondary source
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- USEPA
- Year:
- 1 992
- Bibliographic source:
- NTRL report, OTS0535826, 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- To determine the Acute oral toxicity study of test chemical in Rats.
- GLP compliance:
- not specified
- Test type:
- fixed dose procedure
- Limit test:
- yes
Test material
- Reference substance name:
- 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
- EC Number:
- 201-383-9
- EC Name:
- 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
- Cas Number:
- 81-88-9
- Molecular formula:
- C28H31N2O3.Cl
- IUPAC Name:
- 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthenium chloride
- Test material form:
- solid: particulate/powder
- Details on test material:
- IUPAC name:9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
Mol. formula: C28H31ClN2O3
Molecular Weight: 479.017 gm/mol
Smiles: c1(c2c(oc3c1ccc(\c3)=[N+](/CC)CC)cc(N(CC)CC)cc2)c1c(cccc1)C(O)=O.[ClH-]
InChI: 1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
Constituent 1
- Specific details on test material used for the study:
- No data
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: No data
- Females (if applicable) nulliparous and non-pregnant: [yes/no] No data
- Age at study initiation: No data
- Weight at study initiation: No data
- Fasting period before study: No data
- Housing: No data
- Diet (e.g. ad libitum): Rat pellets
- Water : Distilled water ad libitum
- Acclimation period: No data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data
- Humidity (%): No data
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): No data
IN-LIFE DATES: From: To:No data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Details on oral exposure:
- Maximum dose volume applied: 5000 mg/kg bw
- Doses:
- 5000 mg/kg bw
- No. of animals per sex per dose:
- 5000 mg/kg bw: 10 rats
Control: 10 rats - Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The animals were examined after 2 hours and daily for 14 days.
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight, mortality. - Statistics:
- No data available
Results and discussion
- Preliminary study:
- No data available
Effect levels
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other:
- Remarks:
- Mortality observed
- Mortality:
- Death was observed in one animal at 48 hours and in a second animal at 72 hours.
- Clinical signs:
- other: All animals exhibited a mild to moderate lethargic response accompanied with ataxia, dyspnea, abnormal righting reflex, muscular weakness, piloerection and diarrhea. This condition persisted for approximately seventy-two (72) hours.
- Gross pathology:
- Gross pathology of survivors revealed that all target organs (heart. lungs, spleen. kidneys, liver and gastro-intestinal tract) appeared normal with the exception of a mild renal vascularization.
- Other findings:
- Autopsies of expired animals revealed hyperemic lungs, nephritic kidneys, peripheral lobular hemorrhage of the liver and hemorrhagic gastro-intestinal tract.
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Conclusions:
- In a acute oral toxicity study, the LD50 value was considered to be >5000 mg/kg bw when rats was treated with test chemical.
- Executive summary:
The acute oral toxicity study was performed on rats to determine the toxic nature of the test chemical. 10 Sprague-Dawley rats were treated with test chemical at a concentration of 5000 mg/kg bw by oral intubation route. All animals exhibited a mild to moderate lethargic response accompanied with ataxia, dyspnea, abnormal righting reflex, muscular weakness, piloerection and diarrhea. This condition persisted for approximately seventy-two (72) hours. The average weight gain of the surviving test animals was 67.3 grams and that of control animals was 70.4 grams and the average weight loss of expired test animals was 19.0 grams. Gross pathology of survivors revealed that all target organs (heart. lungs, spleen, kidneys, liver and gastro-intestinal tract) appeared normal with the exception of a mild renal vascularization. Death was observed in one animal at 48 hours and in a second animal at 72 hours. Autopsies of expired animals revealed hyperemic lungs, nephritic kidneys, and peripheral lobular hemorrhage of the liver and hemorrhagic gastro-intestinal tract. Therefore based on the above observations, LD50 was considered to be >5000 mg/kg bw when 10 Sprague-Dawley rats were treated with test chemical orally.
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