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EC number: 271-114-8 | CAS number: 68515-88-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from secondary literature
Data source
Reference
- Reference Type:
- secondary source
- Title:
- Robust summary alkyl sulfide catetgory CAS # 68515-88-8 Genetic Toxicity Elements: Genetic toxicity in vitro
- Author:
- US Environmental Protection Agency
- Year:
- 2 000
- Bibliographic source:
- US Environmental Protection Agency, AR201-12549bZ, 2000
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- In vitro gene toxicity study of Pentene, 2, 4, 4-trimethyl-, sulfurized was studied in Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, TA1538
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Pentene, 2,4,4-trimethyl-, sulfurized
- EC Number:
- 271-114-8
- EC Name:
- Pentene, 2,4,4-trimethyl-, sulfurized
- Cas Number:
- 68515-88-8
- Molecular formula:
- C24H50S8
- IUPAC Name:
- 1-(2,4,4-trimethyl-1-{[(2,4,4-trimethylpentan-2-yl)disulfanyl]disulfanyl}pentan-2-yl)-4-(2,4,4-trimethylpentan-2-yl)tetrasulfane
- Test material form:
- liquid: viscous
- Details on test material:
- - Name of test material (as cited in study report): Pentene, 2,4,4-trimethyl-, sulfurized
- Molecular formula :C24H50S8
- Molecular weight: 595.144 g/mol
- Substance type:Organic
- Physical state:Liquid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material: Pentene, 2, 4, 4-trimethyl-, sulfurized
- EC name: Pentene, 2,4,4-trimethyl-, sulfurized
- Molecular formula: C24H50S8
- Molecular weight: 594.141 g/mole (As in Chem exper)
- Substance type: Organic
- Physical state: No data available
- Purity: 97 %
- Impurities: 3 %
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538
- Details on mammalian cell type (if applicable):
- No aata
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- No data
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 microsomal fraction was isolated from Aroclor 1254-treated rat livers
- Test concentrations with justification for top dose:
- 0, 0.01, 0.03, 0.1, 0.3 and 1 µL/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: The chemical was soluble in DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: 2-anthramine (With S9)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation);
DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: Three replicates
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available
OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available
OTHER: No data available - Rationale for test conditions:
- No data
- Evaluation criteria:
- The plates were observed for a dose dependent increase in the number of revertant colonies
- Statistics:
- The mean number of revertants/plate for three replicate assay plates was calculated for each concentration and strain.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- No data
Applicant's summary and conclusion
- Conclusions:
- Pentene, 2, 4, 4-trimethyl-, sulfurized is non mutagenic at a concentration of 0, 0.01, 0.03, 0.1, 0.3 and 1 µL/plate in Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, TA1538 and hence is negative for gene mutation in vitro.
- Executive summary:
In an in-vitro gene toxicity test, Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538 bacterial cells were exposed to Pentene, 2, 4, 4-trimethyl-, sulfurized in the concentration of 0, 0.01, 0.03, 0.1, 0.3 and 1 µL/plate with and without metabolic activation. Plate incorporation assay was performed with three replicate assay plates. The mean number of his- revertants/plate was calculated for each concentration and strain.The results showed that there was no significant evidence of mutation in any of the Salmonella strains in the quantitative mutagenesis assay in the presence or absence of metabolic activation. Pentene, 2, 4, 4-trimethyl-, sulfurized is non mutagenic at a concentration of 0, 0.01, 0.03, 0.1, 0.3 and 1 µL/plate in Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, TA1538.
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