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EC number: 701-371-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Substance type: dark blue powder
- Physical state: solid
- Impurities (identity and concentrations): moisture and water soluble matter < 1 %
Constituent 1
- Specific details on test material used for the study:
- -Test item name: Blue 10 GN
- Other name in report: Copper, [29H, 31H-phthalocyaninato-(2-)-N29, N30 , N31 , N32]-chlorinated (n=8-13)
- CAS 68987-63-3
-Batch no. 21993
-Purity: 99%
-Description: blue powder
-Storage conditions: Room temperature
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver S9 mix from rats treated with Arochlor 1254
- Test concentrations with justification for top dose:
- 0, 312.5, 625, 1250, 2500 and 5000 μg/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- N-ethyl-N-nitro-N-nitrosoguanidine
- benzo(a)pyrene
- other: 2-Aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
- Evaluation criteria:
- For a substance to be considered positive in this test system, it should
have induced a dose-related and statistically(S) significant increase in
mutation rate (of at least twice the spontaneous reversion rate) in one
or more strains of bacteria in the presence and/or absence of the 59
microsomal enzymes in both experiments at sub-toxic dose levels. If the
two experiments give conflicting results then a third experiment may be
used to confir~· the correct response. Tob~ considered negative the
number of .induced revertants compared to spontaneous revertants should
be less than twofold at each dose level amployed, the intervals of which
should be between 2 and 5 fold and extend to the limits imposed by
toxicity or solubility or up to the maximum recommended dose of 5000
μg/plate. In this case the limiting factor was the maximum recommended
dose. - Statistics:
- not needed
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- No toxicity was exhibited to any of the strains of bacteria used. A precipitate was observed at and above 2500 μg/plate, this did not interfere
with the scoring of revertant colonies. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Experiment 1, without S9 mix | ||||||||||
Test substance concentration per plate | TA100 | TA1535 | WP2uvrA· | TA98 | TA1537 | |||||
0 | 103 | _(113) | 19 | (17) | 24 | (34) | 22 | (22) | 1 1 | (14) |
129 | 12 | 40 | 19 | 1 5 | ||||||
108 | 13.8 | 20 | 4.4 | 37 | 8.5 | 25 | 3.0 | 15 | 2.3 | |
8 | 106 94 |
(104) | 11 20 |
(1 5) | 34 41 |
(37) | 16 20 |
(16) | 1 3 13 |
(14) |
113 | 9.6 | 1 5 | 4.5 | 37 | 3.5 | 1 1 | 4.5 | 16 | 1.7 | |
40 | 117 104 |
(108) | 17 16 |
(15) | 38 41 |
(38) | 25 19 |
(21 ) | 9 1 1 |
(10) |
102 | 8.1 | 13 | 2.1 | 34 | 3.5 | 19 | 3.5 | 1 1 | 1.2 | |
200 | 123 1 12 |
(110) | 20 13 |
(15) | 50 28 |
(40) | 1 5 17 |
(18) | 1 5 12 |
(14) |
95 | 14.1 | 13 | 42 | 11.1 | 22 | 3.6 | 16 | 2.1 | ||
1000 | 107 | (105) | 11 | (10) | 32 | (30) | 22 | (21 ) | 16 | (13) |
1 10 | 6 | 28 | 24 | 13 | ||||||
98 | 6-2 | 13 | 3.6 | 29 | 2.1 | 16 | 4.2 | 9 | 3.5 | |
5000 | 99P 124P |
(107) | 13P 13P |
(14) | 25P 24P |
(28) | 13P 19P |
(17) | 8P 1 1p |
(10) |
99P | 14.4 | 17P | 2.3 | 36P | 6.7 | 19P | 3.5 | 1 2P | 2.1 | |
Positive control | ENNG | ENNG | ENNG | 4NQO | 9AA | |||||
Concentration (ug/plate) |
3 | 5 | 2 | 0.2 | 80 | |||||
579 | 197 | 203 | 142 | 416 | ||||||
594 | (550) | 173 | (182) | 190 | (192) | 123 | (132) | 342 | (378) | |
476 | 64.2 | 176 | 13.1 | 184 | 9.7 | 130 | 9.6 | 375 | 37.1 |
P = precipitation
Applicant's summary and conclusion
- Conclusions:
- non mutagenic in the Ames test
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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