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EC number: 617-081-9
CAS number: 80473-92-3
The purpose of this test was to evaluate the corrosivity potential of
the test item using the SkinEthic in vitro Reconstructed Human Epidermal
(RHE, SkinEthic Laboratories, Lyon, France) model after treatment
periods of 3 and 60 minutes. Corrosive test items are able to penetrate
the stratum corneum and are sufficiently cytotoxic to cause cell death
in the underlying cell layers. Toxicity is determined by the metabolic
reduction of the yellow tetrazolium salt
(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) to a
blue formazan dye by mitochondrial succinate dehydrogenase by viable
cells in the test item treated tissues relative to the negative control
Duplicate tissues were treated with the test item for exposure periods
of 3 and 60 minutes. At the end of the exposure period the test item was
rinsed from each tissue before each tissue was taken for MTT-loading.
After MTT loading each tissue was placed in 1.5 mL Isopropanol for MTT
extraction. At the end of the formazan extraction period the tissues
were mixed thoroughly and triplicate 200 μL samples were transferred to
the appropriate wells of a pre-labeled 96-well plate. The optical
density (OD) was measured at 562 nm (OD562). Data are presented in the
form of percentage viability (MTT reduction in the test item treated
tissues relative to negative control tissues).
The relative mean viabilities of the test item treated tissues were: 3
minutes exposure : 93.1% 60 minutes exposure : 98.3%
Quality criteria: The quality criteria required for acceptance of
results in the test were satisfied.
The test item was considered to be non-corrosive to the skin.
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