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EC number: 202-058-4 | CAS number: 91-30-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- calculation (if not (Q)SAR)
- Remarks:
- Migrated phrase: estimated by calculation
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from computational model developed by USEPA
- Qualifier:
- according to guideline
- Guideline:
- other: Modeling database
- Principles of method if other than guideline:
- The Biodegradation Probability Program (BIOWIN) estimates the probability for the rapid aerobic biodegradation of an organic chemical in the presence of mixed populations of environmental microorganisms .The model is part of the EpiSuite program of the US-EPA. Estimations are made with BIOWIN version 4.10Estimates are based upon fragment constants that were developed using multiple linear and non-linear regression analyses. Experimental biodegradation data for the multiple linear and non-linear regressions were obtained from Syracuse Research Corporation's (SRC) data base of evaluated biodegradation data (Howard et. al., 1987). This version (v4.10) designates the models as follows (see also Boethling et al. 2003):
Biowin1 = linear probability model
Biowin2 = nonlinear probability model
Biowin3 = expert survey ultimate biodegradation model
Biowin4 = expert survey primary biodegradation model
Biowin5 = MITI linear model
Biowin6 = MITI nonlinear model
Biowin7 = anaerobic biodegradation model - GLP compliance:
- not specified
- Oxygen conditions:
- other: aerobic (Biowin 1-6) and anaerobic (Biowin 7)
- Inoculum or test system:
- other: mixed populations of environmental microorganisms
- Duration of test (contact time):
- 2.509 mo
- Details on study design:
- Using the computer tool BIOWIN v4.10 by US-EPA (EPIWIN) the aerobic as well as the anaerobic biodegradability of the test material can be estimated. The follwoing seven different models are used by the tool: Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (calles Biowin 1-7, respectively). Due to this results the overall prediction of readily biodegradability is done for the desired chemical.
- Parameter:
- other: Half-life
- Value:
- 50
- Sampling time:
- 2.509 mo
- Remarks on result:
- other: Other details not known
- Details on results:
- Biowin1 (Linear Model Prediction) : 0.0585: Does Not Biodegrade Fast
Biowin2 (Non-Linear Model Prediction): 0.0221: Does Not Biodegrade Fast
Biowin3 (Ultimate Biodegradation Timeframe): 2.5094: Weeks-Months
Biowin4 (Primary Biodegradation Timeframe): 3.2762: Days-Weeks
Biowin5 (MITI Linear Model Prediction) : -0.3017: Does Not Biodegrade Fast
Biowin6 (MITI Non-Linear Model Prediction): 0.0027: Does Not Biodegrade Fast
Biowin7 (Anaerobic Model Prediction): -0.6416: Does Not Biodegrade Fast
Ready Biodegradability Prediction: NO - Validity criteria fulfilled:
- not specified
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The biodegradability of the substance was calculated using seven different Biowin 1-7 models of the BIOWIN v4.10 software. The results indicate that Benzenesulfonic acid, 5-amino-2-(phenylamino)- is not expected to be readily biodegradable.
- Executive summary:
Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound Benzenesulfonic acid, 5-amino-2-(phenylamino)- (CAS no. 91 -30 -5) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that Benzenesulfonic acid, 5-amino-2-(phenylamino)- is not expected to be readily biodegradable.
Reference
BIOWIN (v4.10) Program Results:
==============================
SMILES : O=S(=O)(O)c(c(Nc(cccc1)c1)ccc2N)c2
CHEM : Benzenesulfonic acid, 5-amino-2-(phenylamino)-
MOL FOR: C12 H12 N2 O3 S1
MOL WT : 264.30
--------------------------- BIOWIN v4.10 Results ----------------------------
Biowin1 (Linear Model Prediction) : Does Not Biodegrade Fast
Biowin2 (Non-Linear Model Prediction): Does Not Biodegrade Fast
Biowin3 (Ultimate Biodegradation Timeframe): Weeks-Months
Biowin4 (Primary Biodegradation Timeframe): Days-Weeks
Biowin5 (MITI Linear Model Prediction) : Does Not Biodegrade Fast
Biowin6 (MITI Non-Linear Model Prediction): Does Not Biodegrade Fast
Biowin7 (Anaerobic Model Prediction): Does Not Biodegrade Fast
Ready Biodegradability Prediction: NO
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin1 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2338 | -0.4675
Frag | 1 | Sulfonic acid / salt -> aromatic attach | -0.2238 | -0.2238
Frag | 1 | Unsubstituted phenyl group (C6H5-) | 0.1281 | 0.1281
MolWt| * | Molecular Weight Parameter | | -0.1258
Const| * | Equation Constant | | 0.7475
============+============================================+=========+=========
RESULT | Biowin1 (Linear Biodeg Probability) | | 0.0585
============+============================================+=========+=========
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin2 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.9070 | -3.8140
Frag | 1 | Sulfonic acid / salt -> aromatic attach | -1.0283 | -1.0283
Frag | 1 | Unsubstituted phenyl group (C6H5-) | 1.7991 | 1.7991
MolWt| * | Molecular Weight Parameter | | -3.7531
============+============================================+=========+=========
RESULT | Biowin2 (Non-Linear Biodeg Probability) | | 0.0221
============+============================================+=========+=========
A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast
A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin3 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1349 | -0.2699
Frag | 1 | Sulfonic acid / salt -> aromatic attach | 0.1422 | 0.1422
Frag | 1 | Unsubstituted phenyl group (C6H5-) | 0.0220 | 0.0220
MolWt| * | Molecular Weight Parameter | | -0.5841
Const| * | Equation Constant | | 3.1992
============+============================================+=========+=========
RESULT | Biowin3 (Survey Model - Ultimate Biodeg) | | 2.5094
============+============================================+=========+=========
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin4 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1084 | -0.2168
Frag | 1 | Sulfonic acid / salt -> aromatic attach | 0.0216 | 0.0216
Frag | 1 | Unsubstituted phenyl group (C6H5-) | 0.0049 | 0.0049
MolWt| * | Molecular Weight Parameter | | -0.3813
Const| * | Equation Constant | | 3.8477
============+============================================+=========+=========
RESULT | Biowin4 (Survey Model - Primary Biodeg) | | 3.2762
============+============================================+=========+=========
Result Classification: 5.00 -> hours 4.00 -> days 3.00 -> weeks
(Primary & Ultimate) 2.00 -> months 1.00 -> longer
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin5 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1577 | -0.3154
Frag | 1 | Sulfonic acid / salt -> aromatic attach | 0.0221 | 0.0221
Frag | 8 | Aromatic-H | 0.0082 | 0.0657
MolWt| * | Molecular Weight Parameter | | -0.7863
Const| * | Equation Constant | | 0.7121
============+============================================+=========+=========
RESULT | Biowin5 (MITI Linear Biodeg Probability) | | -0.3017
============+============================================+=========+=========
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin6 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.2264 | -2.4527
Frag | 1 | Sulfonic acid / salt -> aromatic attach | 0.6780 | 0.6780
Frag | 8 | Aromatic-H | 0.1201 | 0.9611
MolWt| * | Molecular Weight Parameter | | -7.6300
============+============================================+=========+=========
RESULT |Biowin6 (MITI Non-Linear Biodeg Probability)| | 0.0027
============+============================================+=========+=========
A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable
A Probability Less Than 0.5 indicates --> NOT Readily Degradable
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin7 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2778 | -0.5556
Frag | 1 | Sulfonic acid / salt -> aromatic attach | -0.3768 | -0.3768
Frag | 1 | Unsubstituted phenyl group (C6H5-) | 0.2182 | 0.2182
Frag | 8 | Aromatic-H | -0.0954 | -0.7634
Const| * | Equation Constant | | 0.8361
============+============================================+=========+=========
RESULT | Biowin7 (Anaerobic Linear Biodeg Prob) | | -0.6416
============+============================================+=========+=========
A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast
A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast
Ready Biodegradability Prediction: (YES or NO)
----------------------------------------------
Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey
model) result is "weeks" or faster (i.e. "days", "days to weeks", or
"weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then
the prediction is YES (readily biodegradable). If this condition is not
satisfied, the prediction is NO (not readily biodegradable). This method
is based on application of Bayesian analysis to ready biodegradation data
(see Help). Biowin5 and 6 also predict ready biodegradability, but for
degradation in the OECD301C test only; using data from the Chemicals
Evaluation and Research Institute Japan (CERIJ) database.
Description of key information
Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound Benzenesulfonic acid, 5-amino-2-(phenylamino)- (CAS no. 91 -30 -5) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that Benzenesulfonic acid, 5-amino-2-(phenylamino)- is not expected to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Various predicted data for the test compound Benzenesulfonic acid, 5 -amino-2 -(phenylamino)- (CAS No. 91-30-5) and for its read across substance were reviewed for the biodegradation end point which are summarized as below:
Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound Benzenesulfonic acid, 5-amino-2-(phenylamino)- (CAS no. 91 -30 -5) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that Benzenesulfonic acid, 5-amino-2-(phenylamino)- is not expected to be readily biodegradable.
Biodegradation study of read across substance 4-Amino-1,1’-azobenzene-3,4'-disulfonic acid (acid yellow 9) (CAS no. 101-50-8) was performed using 15 different Streptomyces spp. for 14 days (A. Paszcyzynski, et. al; 1991) . The test was performed using 15 different Streptomyces spp. as an inoculum. The read across substance conc. used is 50 mg/l. Each Streptomyces spp. was grown in a cotton-plugged 250 ml flask containing 25 ml of the following medium: 0.2 M Tris buffer (pH 7.6) 100 ml, 1.0g vitamin-free Casamino acids, 100 µg thiamine, 100 µg biotin, 2 g D-glucose, 900 ml deionized wate. Thiamine, biotin, and D-glucose were filter sterilized and added to the autoclaved medium. The dye was filter sterilized and added at 0.005% (w/v) to the autoclaved basal medium. Cultures were incubated at 37ᵒC for 14 days with shaking (200 rev min1). Three replicates for each strain grown in only the basal medium were incubated as well.Degradation of read across substance was determined by spectrophotometrically and by using HPLC.
The percentage biodegradation of read across substance was found to be 0% i.e; no degradation of test substance was observed by the 15 different Streptomyces spp. Thus, the substance 4-Amino-1,1’-azobenzene-3,4'-disulfonic acid was determined to be non-biodegradable.
Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of read across substance 2-Amino-8-naphthol-6-sulfonic acid (CAS no. 90-51-7) (J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 30 mg/l and initial substance conc. used in the study was 100 mg/l. The read across substance is determined to be 5.3 % biodegraded in water after 2 wk (14 days) on the basis of O2 consumption, BOD. 0.4% by TOC removal and <0 % by (UVVIS) parameters. Hence, the substance 2-Amino-8-naphthol-6-sulfonic acid is considered to be not readily biodegradable in water.
A closed bottle test according to OECD 301D guidelines was performed to determine the ready biodegradability of 7-Amino-4 hydroxy- 2 – naphthalenesulphonic acid (CAS no. 87-02-5) (GREIM H.; et. al, 1994). No degradation of read across chemical 7-Amino-4 hydroxy- 2 – naphthalenesulphonic acid was observed in 28 days. Therefore, the chemical was considered to be not readily biodegradable.
Biodegradation screening test was conducted for 14 days (2 weeks) for evaluating the percentage biodegradability of the read across substance 7-Amino-4 hydroxy- 2 - naphthalenesulphonic acid (CAS no. 87-20 -5) (J-CHECK, 2016). Concentration of inoculum i.e, sludge is 30 mg/l and initial substance conc. used in the study is 100 mg/l. The percentage degradation of read across substance is determined to be 6.3% by BOD, 8.4% by TOC and 0% by UV-VIS Thus, the substance 7-Amino-4 hydroxy- 2 - naphthalenesulphonic acid was considered to be not readily biodegradable in water.
A batch test in an open system was conducted for evaluating the biodegradability of 1-Naphthylamine-6-sulphonic acid (CAS no. 119-79-9) (P. Pitter, 1976). The compound was the sole source of organic carbon for the microbes of the inoculum. Degradation was studied by determining COD (organic carbon). The pH of the biological medium was 7.2. Initial concentration; 125 - 200mg/l COD. Inoculum; Activated sludge, adapted for 20 days, fed by glucose and peptone with simultaneous addition of the substance tested in the concentration up to 125 mg/l. The sludge age was 5 days. Concentration of the inoculum; 100mg/l. Average temperature during the experiment; 20°C. Under the test conditions the compound was evaluated to be biologically hard to decompose(non-biodegradable).
Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of read across substance 2-Amino-1,5-naphthalenedisulfonic acid (CAS no. 117-62-4) (J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 30 mg/l and initial substance conc. used in the study was 100 mg/l. The percentage degradation of read across substance was determined to be 1% by BOD, 0% by HPLC and 5% by TOC removal in 28 days. Thus, the substance 2-Amino-1,5-naphthalenedisulfonic acid is not expected to be readily biodegradable in water.
Biodegradation study was conducted for 30 days for evaluating the percentage biodegradability of read across substance1,3-Naphthalenedisulfonic acid, 6-amino- (CAS no. 118-33-2) (GREIM H.; et. al, 1994). No degradation of read across substance was determined by BOD in 30 days. Thus, the substance1,3-Naphthalenedisulfonic acid, 6-amino- is not expected to be readily biodegradable in water.
On the basis of above results for target and read across substance, it can be concluded that the test substance Benzenesulfonic acid, 5-amino-2-(phenylamino)- is likely to be not readily biodegradable in nature.
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