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EC number: 258-751-7 | CAS number: 53767-93-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- TA102 strain is missing
- Justification for type of information:
- In addition to the Ames test on Dihydromyrcenyl acetate, Ames information from Dihdyromyrcenol is added to make up for the missing TA102 strain. The read across rationale is presented in the Genetic toxicity Endpoint summary, the accompanying files are also attached there.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Ames test method et al. (1975)
- Version / remarks:
- Ames test method et al. (1975)
- GLP compliance:
- no
- Remarks:
- conduected prior to the GLP guideline
- Type of assay:
- bacterial forward mutation assay
Test material
- Reference substance name:
- 2,6-dimethyloct-7-en-2-yl acetate
- EC Number:
- 258-751-7
- EC Name:
- 2,6-dimethyloct-7-en-2-yl acetate
- Cas Number:
- 53767-93-4
- Molecular formula:
- C12H22O2
- IUPAC Name:
- 2,6-dimethyloct-7-en-2-yl acetate
- Test material form:
- liquid
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- 0, 0.008, 0.04, 0.2, 1.0 and 5.0 microL/plate
- Vehicle / solvent:
- Appropriate dilutions in DMSO were prepared immediately before use.
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- 2 ml molten soft agar were added to 0.1 mL of a fully grown culture of one of the tester strains and 0.1 mL of the appropriate dilution /suspension of the test compound (and 0.5 mL of the S-9 mix if indicated). The ingredients were thoroughly mixed and immediately poured into minimal glucose agar plates. After the top agar had been allowed to harden, the plates were incubated at 37°C for two days. Then the colonies (revertants which are histidine-independant) were counted, and the background lawn of baterial growth examined microscopically. All determination were made in triplicate and appropriate controls were included in each assay.
Results and discussion
Test results
- Key result
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 1.0 and 5.0 microL/plate DHMA was clearly toxic to strains TA1537, TA1538, TA98 and TA100 in the absence of S9
- Vehicle controls validity:
- not valid
- Positive controls validity:
- valid
- Additional information on results:
- Incorporation of 0.008 up to 0.5 - 0.5 microL of the test liquid par plate did not induce an increase in the number of his +revertants with any of the five tester strains either in the presence ot absence of S-9 mix. At 1.0 and 5.0 microL/plate DHMA was clearly toxic to strains TA1537, TA1538, TA98 and TA100 in the basence of S9 mix as revealed by a less dense background lawn of bacterial growth.
Applicant's summary and conclusion
- Conclusions:
- From the present results it can be concluded that the substance up to non- inhibitory levels did not reveal any mutagenic activity in the plate incorporation assay with S.Typhimurium TA1535, TA1537, TA1538, TA98 or TA100 in the presence or absence of the liver microsome activation system under the test condition employed in this evaluation.
- Executive summary:
The mutagenic activity of the substance was examined in the Salmonella/microsome mutagenicity test, using a set of five histidine requiring mutants of S. Typhimurium (TA1535, TA1537, TA1538, TA98 and TA100) and liver homogenate of Aroclor induced rats. Incorporation of the test liquid up to non-inhibitory levels i.e. 0.2 - 5.0 microl/plate did not increase the number of his +revertants in any of the five tester strains either in the presence or in the absence of the liver microsome activation system.
It was concluded that the present results did not reveal any mutagenic activity of the test material in the Salmonella/microsome metagenicity test.
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