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EC number: 203-851-8 | CAS number: 111-26-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of the Genetic Toxicity of Synthetic Chemicals (XIV)-in vitro Chromosomal Aberration Assay with 11 Chemicals in Chinese Hamster Lung Cells.
- Author:
- Youn-Jung Kim & Jae-Chun Ryu
- Year:
- 2 006
- Bibliographic source:
- MOLECULAR & CELLULAR TOXICOLOGY, Vol. 2, No. 2, 89-96, June 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Principles of method if other than guideline:
- Evaluation of the Genetic Toxicity of Synthetic Chemicals (XIV)-in vitro Chromosomal Aberration Assay with 11 Chemicals in Chinese Hamster Lung Cells. One of the chemicals studied include is n-Hexylamine
- GLP compliance:
- not specified
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- Hexylamine
- EC Number:
- 203-851-8
- EC Name:
- Hexylamine
- Cas Number:
- 111-26-2
- Molecular formula:
- C6H15N
- IUPAC Name:
- hexan-1-amine
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material: Hexylamine
- Molecular formula: C6H15N
- Molecular weight:101.19 g/mol
- Smiles notation (if other than submission substance): C(CCC)CCN
- InChl: 1S/C6H15N/c1-2-3-4-5-6-7/h2-7H2,1H3
- Substance type: Organic
- Physical state: liquid
Constituent 1
Method
- Target gene:
- Chinese Hamster Lung Cells
Species / strain
- Species / strain / cell type:
- other: Chinese Hamster Lung Cells
- Details on mammalian cell type (if applicable):
- - Type and identity of media: Monolayer media with EMEM supplemented with 10% FBS, 50 units/mL penicillin and 50 μg/mL streptomycin.
- Properly maintained: Yes, maintained at 370 C in humidified 5% CO2 atmosphere. - Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 activation
- Test concentrations with justification for top dose:
- 36.3, 72.5, 144.9, 160.3, 320.5 and 640.9 μg/mL
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Dimethylsulfoxide (DMSO) were used.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: Cyclophosphamide and mitomycin C (MMC)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar (plate incorporation); preincubation; in suspension; as impregnation on paper disk: Dose were prepared and separately added to 3-day-old Eagles minimum essential medium (EMEM) cultures (approximately 105 cells/60 mm dish).
DURATION
- Preincubation period: No data available
- Exposure duration: 6 hr and 24 hr
- Expression time (cells in growth medium):
- Selection time (if incubation with a selection agent): 22 hr followed by addition of medium containing colcemid
- Fixation time (start of exposure up to fixation or harvest of cells): 2hr
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: No data available
NUMBER OF CELLS EVALUATED: few drop of cell were evaluated for chromosomal aberrations.
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: Yes,
For dose range-finding study, study performed in the absence of a rat liver S9 activation system. For the growth inhibition assay, CHL cells were seeded at the density of 5×104 cells/mL into 96 well plates. 24 hr after seeding, several different doses of sample were separately added and incubated for 6 hr in the presence of S9 activation system and 24 hr in the absence of S9 system. And then the 50% inhibition concentration (IC50) values were calculated by MTT assay.
OTHER EXAMINATIONS:
- Determination of polyploidy: Yes
- Determination of endoreplication: Yes
- Other: No data available
OTHER: No data available - Evaluation criteria:
- Absence and presence of chromosome aberrations
- Statistics:
- Data were analyzed by using Fishers exact test48 with Dunnetts adjustment and compared with results from the solvent controls.
Data from count up well-spread 200 metaphase cells were expressed as percentages, and then dose-dependent responses and the statistical significance in p-value will be considered as positive results.
Results and discussion
Test results
- Species / strain:
- other: Chinese Hamster Lung Cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: Dose finding study, result not mention
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: Yes, performed in the absence of a rat liver S9 activation system. For the growth inhibition assay, CHL cells were seeded at the density of 5×104 cells/mL into 96 well plates. 24 hr after seeding, several different doses of sample were separately added and incubated for 6 hr in the presence of S9 activation system and 24 hr in the absence of S9 system. And then the 50% inhibition concentration (IC50) values were calculated by MTT assay.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without
n-Hexylamine in the concentration of 36.3, 72.5, 144.9, 160.3, 320.5 and 640.9 μg/mL did not show any evidence of gene toxicity when CHL cells were exposed to the test chemical and hence is not likely to classify as a gene mutant in vitro. - Executive summary:
In a gene toxicity test, Chinese Hamster Lung Cells (CHL) cells were exposed to n-Hexylamine at a concentration of 36.3, 72.5, 144.9, 160.3, 320.5 and 640.9 μg/mL with and without metabolic activation for 6 and 24 hours. The results showed that there was no significant evidence of chromosome aberrations after treatment. Independently of tested n-Hexylamine concentration, the results showed no evidence of gene toxicity. Therefore, it is considered that n-Hexylamine in the concentration of 144.9 μg/mL without S9 and 640.9 μg/mL with S9 does not cause genetic chromosome aberrations when CHL cells are exposed to the test chemical in the absence and inpresence of S9 activation system of S9 system for 6 and 24 hr. Hence the test chemical is not liekly to classify as a gene mutant in vitro.
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