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EC number: 281-064-9 | CAS number: 83846-85-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to generally accepted guidelines and in accordance with GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- Room temperature dropped below 19.5oC, to as low as 18oC. This deviation is not considered to have had an advserse effect on study given that all validity criteria were met.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Severn Trent Water sewage treatment plant, Loughborough, UK
- Storage conditions: Continuous aeration at ambient laboratory temperature, approximately 21oC
- Storage length: Used on day of collection
- Preparation of inoculum for exposure: Seage sludge washed twice by settlement and resuspension in mineral medium. Washed sample maintained on continuous aeration at approximately 21oC.
- Concentration of sludge: Suspended solids concentration equal to 4.6 g/L - Duration of test (contact time):
- ca. 56 d
- Initial conc.:
- ca. 12.7 mg/L
- Based on:
- ThOD/L
- Details on study design:
- TEST CONDITIONS
- Composition of medium: as recommended in OECD guidelines
- Test temperature: 22 +/-2oC
- pH: 7.5
- pH adjusted: Yes
- Aeration of dilution water: CO2-free air bubbled through solutions at 30-100 mL/min
- Suspended solids concentration: 30 mg/L (nominal)
- Continuous darkness: Yes
TEST SYSTEM
- Culturing apparatus: 5L culture vessels
- Number of culture flasks/concentration: Duplicate test vessels
- Method used to create aerobic conditions: CO2-free air bubbled through solutions at 30-100 mL/min
- Test performed in open system: No
- Details of trap for CO2 and volatile organics if used: Two 500 mL Dreschel bottles containing 0.05 M NaOH in purified water.
SAMPLING
- Sampling frequency: 0,2,6,8,10,14,21,28,35,42,49,56 and 57 days
- Sampling method: Samples taken from first CO2 absorber vessel. Absorber vessel 2 sampled days 0 and 57
- Sample storage before analysis: None
CONTROL AND BLANK SYSTEM
- Inoculum blank: Mineral medium only
- Procedure control: Sodium benzoate (17.1 mg/L), equivalent to 10 mg C/L
- Toxicity control: Test substance (12.7 mg/L) plus sodium benzoate (17.1 mg/L), equivalent to 20 mg C/L in total - Reference substance:
- other: Sodium benzoate
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 10
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 36
- Sampling time:
- 56 d
- Results with reference substance:
- The reference substance 90 and 93% degradation after 28 and 56 days, respectively.
The toxicity control showed 47 and 48% degradation after 28 and 56 days, respectively. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the test substance is considered not to be readily biodegradable
- Executive summary:
A study was performed to assess the ready biodgradability of the test substance in accordance with the OECD guideline 301B and EU method C.4 -C, in compliance with GLP.
The test substance in a mineral medium was inoculated with domestic sewage effluent and incubated for 56 days under aerobic conditions.
The test substance showed 10 and 36% degradation after 28 and 56 days, respectively. Therefore, the test substance is classified as not readily biodegradable under the conditions of the study.
The reference substance 90 and 93% degradation after 28 and 56 days, respectively, confirming the suitability of the inoculum and test conditions.
The toxicity control showed 47 and 48% degradation after 28 and 56 days, respectively, confirming the test substance did not have an inhibitory effect on the sewage treatment micro-organisms used in the study.
This study can be classified as an acceptable key study and satisfies the guideline requirement for a biodegradation in water screening test.
Reference
Day |
% biodegradation |
||
Test substance |
Procedure control |
Toxicity control |
|
8 |
0 |
84 |
41 |
14 |
0 |
92 |
37 |
21 |
2 |
91 |
38 |
28 |
10 |
90 |
47 |
35 |
12 |
83 |
44 |
42 |
18 |
88 |
44 |
49 |
22 |
80 |
43 |
56 |
33 |
101 |
46 |
57* |
36 |
93 |
48 |
* Day 57 values corrected to include any carry-over of CO2detected in Absorber 2 |
The concentration of the test substance did not show toxic effects to the seage micro-organisms used in the test. The toxicity control had 47 and 48% degradation after 28 and 56 days, respectively.
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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