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EC number: 287-466-0 | CAS number: 85508-41-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- 15 min of sonication of diluted solution from a stock solution of 0.1 g/L
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 261 mg/L CaCO3
- Test temperature:
- 20 °C
- pH:
- 8.3
- Dissolved oxygen:
- 97%
- Nominal and measured concentrations:
- Nominal: 0, 0.9, 1.9, 4.3, 9.4, 21, 45 and 100 mg/L
- Details on test conditions:
- 10 Daphnids for each concentration in duplicate
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 47 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- ca. 4.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 47 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 48 h EC0, 50 and 100 for Daphnia magna were determined to be 4.3, >100 and >100 mg/L (nominal), respectively, equivalent to 2.0, >47 and >47 mg a.i./L, respectively.
- Executive summary:
A study was conducted to determine the toxicity of the test substance (46.9% purity) to aquatic invertebrates (Daphnia magna) according to OECD Guideline 202. Groups of 10 Daphnia were exposed for 48 h under static conditions at nominal concentrations of 0, 0.9, 1.9, 4.3, 9.4, 21, 45 and 100 mg/L, equivalent to 0, 0.4, 0.9, 2.0, 4.4, 10, 21 and 47 mg a.i./L. The swimming mobility was assessed after 24 and 48 h. No analytical monitoring of test concentrations was conducted. Test material was observed to fall out of solution as concentrations increased, due to the hardness of the test medium. Under the study conditions, the 48 h EC0, 50 and 100 were determined to be 4.3, >100 and >100 mg/L (nominal), respectively, equivalent to 2.0, >47 and >47 mg a.i./L, respectively (Böttcher, 1997).
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 08-05-1990 to 22-06-1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- Cremophor
- Details on test solutions:
- The test substance was dissolved in cremophore and the diluted with deionised water. After the dispersion, the solution is stirred mechanically to produce the stock solution. The stock solution contains 500 mg/L of the test substance.
A series of dilution was prepared with the diltuion water. 10 mL of each corresponding dilution was added per test bottle. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test strain of Daphnia magna Straus was obtained from the Institute National de Recherche Chimique Appliquee, France. The strain is being cultured since 1978 in the Laboratory for Environmental Analysis and Ecology, BASF AG Ludwigshafen.
Age of the animals: 2-24 h - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.82 mmol/L
- Test temperature:
- 19-21°C
- pH:
- 7.96-8.2
- Dissolved oxygen:
- 8.01-8.94
- Conductivity:
- 600-700 µSiemens/cm
- Nominal and measured concentrations:
- - Nominal: 0, 0.07, 0.2, 0.4, 0.8, 1.6, 3.1, 6.2, 13, 25, 50 and 100 mg test substance/L
- Details on test conditions:
- TEST SYSTEM
- Aeration: The test water is aerated with oxygen-free air until oxygen saturation and then left to stand for at least 24 h.
- No. of organisms per vessel: five
- No. of vessels per concentration (replicates): four
- No. of vessels per control (replicates): four
- No. of vessels per vehicle control (replicates): four
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water
- Ca/mg ratio: 4:1
- Na/K ratio: 10:1
- Conductivity: 600-700 µSiemens/cm
OTHER TEST CONDITIONS
- Photoperiod: 16 h light: 8 h dark - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 1.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14.36 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 40 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 48 h EC0, 50, and 100 (in terms of swimming mobility) of the test substance were determined to be 1.56, 14.36 and 100 mg test substance/L (nominal), respectively.
- Executive summary:
A study was conducted to determine the toxicity of the test substance (40% purity) to aquatic invertebrates (Daphnia magna Straus) according to Method C.2. The Daphnia were exposed for 48 h at nominal concentrations up to 100 mg/L under static conditions. The swimming mobility was assessed after 0, 3, 24 and 48 h. No analytical monitoring of test concentrations was conducted. The average water pH was 7.78 and the water hardness 2.83 mmol/L. Under the study conditions, the nominal 48 h EC0, 50 and 100 based on mobility were determined to be 1.6, 14 and 100 mg test substance/L (nominal), equivalent to 0.6, 5.7 and 40 mg a.i./L (Jatzek, 1990).
Referenceopen allclose all
Replicate | Replicate | Concentration mg/l | 24h | 48h | % immobility | ||
1 | 2 | blank | 0 | 0 | 0 | 0 | 0 |
29 | 30 | 0.9 | 0 | 0 | 0 | 0 | 0 |
31 | 32 | 1.9 | 0 | 0 | 0 | 0 | 0 |
33 | 34 | 4.3 | 0 | 0 | 0 | 0 | 0 |
35 | 36 | 9.4 | 0 | 2 | 4 | 5 | 45 |
37 | 38 | 21 | 0 | 0 | 3 | 2 | 25 |
39 | 40 | 45 | 0 | 1 | 4 | 3 | 35 |
100 | 0 | 0 | 1 | 2 | 20 |
Description of key information
The structural analogue substance Disperse Blue 79:1 Br was used in two tests to determine the short-term toxicity to aquatic invertebrates. The test conducted with the highest substance purity was selected as key study: 48-h EC50 to Daphnia magna > 100 mg/L, nominal (OECD 202). The EC50 is far above the water solubility limit of both source and target chemical.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Remarks:
- 48 h
- Effect concentration:
- > 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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