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EC number: 445-630-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-11-21 - 2003-05-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline Study (OECD)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- -
- EC Number:
- 445-630-0
- EC Name:
- -
- Molecular formula:
- Unspecified
- IUPAC Name:
- 1-(acetyloxy)but-3-en-2-yl acetate; 2-hydroxybut-3-en-1-yl acetate
- Details on test material:
- - Name of test material (as cited in study report): Vinylglykoldiacetat 75% ig
- Test substance No.: 02/0526-1
- Date of manufacture: October 21, 2001
- Physical state: Colorless liquid
- Analytical purity: 74.8 area %
- Lot/batch No.: Kanister 1, 2, 4, 5
- Storage condition of test material: Room temperature (N2 conditions)
Constituent 1
Method
- Target gene:
- Histidine operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S9-mix
- Test concentrations with justification for top dose:
- Standard plate test: 0, 27, 135, 675, 3375, 6750 µg/plate
Preincubation test: 0, 5.4, 27, 135, 675, 3.375 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene; 2.5 µg/plate, dissolved in DMSO for TA 1535, TA 100, TA 1537, TA 98; 60 µg/plate, dissolved in DMSO for E. coli WP2 uvrA
- Remarks:
- with metabolic activation
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N'-nitro-N-nitrosoguanidine (MNNG); 5 µg/plate, dissolved in DMSO for strains TA 1535, TA 100
- Remarks:
- without metabolic activation
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylendiamine (NOPD); 10 µg/plate, dissolved in DMSO for strain TA 98
- Remarks:
- without metabolic activation
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without metabolic activation
Migrated to IUCLID6: 100 µg/plate, dissolved in DMSO for strain TA 1537
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- without metabolic activation
Migrated to IUCLID6: 5 µg/plate, dissolved in DMSO for strain E. coli WP2 uvrA
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation); preincubation
DURATION
- Preincubation period: 20 min, 37°C
- Exposure duration: 48 - 72 h, 37°C
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: decrease in the number of revertants, clearing or diminution of the background lawn, reduction in the titer - Evaluation criteria:
- - The test chemical is considered positive in this assay if the following criteria are met:
A dose-related and reproducible increase in the number of revertant colonies, i.e. about doubling of the spontaneous mutation rate in at least one tester strain either without S-9 mix or after adding a metabolizing system.
- A test substance is generally considered nonmutagenic in this test if:
The number of revertants for all tester strains were within the historical negative control range under all experimental conditions in two experiments carried out independently of each other.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No test substance precipitation was found
RANGE-FINDING/SCREENING STUDIES:
The experimental procedure was based on the method described by Yahagi et al. (1977) and
Matsushima et al . (1980).
0.1 ml test solution or vehicle, 0.1 ml bacterial suspension and 0 .5 ml S-9 mix or phosphate buffer were incubated at 37'C for the duration of about 20 minutes using a shaker. Subsequently, 2 ml of soft agar was added and, after mixing, the samples are poured onto the agar plates within approx. 30 seconds. After incubation at 37'C for 48 - 72 hours in the dark, the bacterial colonies were counted.
ADDITIONAL INFORMATION ON CYTOTOXICITY:
A weak bacteriotoxic effect (slight decrease in the number of revertants, slight reduction
in the titer) was observed in the standard plate test depending on the strain and test
conditions at doses > 3,375 pg/plate .
In the preincubation assay bacteriotoxicity (reduced background growth, slight decrease in the number of revertants, slight reduction in the titer) was observed depending on the strain and test conditions at doses > 675 pg/plate. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Results standard plate experiment:
Dose µg/plate | metabolic activation | TA98 | TA100 | TA1535 | TA1537 | E.coli WP2 uvrA | ||||||||||
Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | ||
0 | + | 44 | 40 | 34 | 102 | 111 | 106 | 16 | 14 | 19 | 12 | 8 | 12 | 28 | 32 | 34 |
27 | + | 41 | 37 | 37 | 110 | 105 | 100 | 17 | 14 | 21 | 16 | 14 | 11 | 30 | 32 | 30 |
135 | + | 39 | 39 | 36 | 106 | 104 | 110 | 23 | 19 | 20 | 15 | 11 | 11 | 20 | 31 | 26 |
675 | + | 30 | 33 | 36 | 102 | 91 | 103 | 18 | 15 | 17 | 7 | 7 | 7 | 29 | 24 | 31 |
3375 | + | 26 | 37 | 14 | 95 | 90 | 94 | 14 | 18 | 14 | 11 | 12 | 10 | 20 | c | 24 |
6750 | + | 17 | 16 | 16 | 70 | 53 | 49 | 8 | 11 | 8 | 6 | 7 | 4 | 17 | 16 | 19 |
2.5 µg 2-Aminoanthracene | + | 864 | 899 | 838 | 902 | 931 | 874 | 190 | 134 | 146 | 111 | 124 | 131 | |||
60 µg 2-Aminoanthracene | + | 250 | 264 | 134 | ||||||||||||
0 | - | 28 | 25 | 26 | 100 | 107 | 103 | 18 | 14 | 17 | 9 | 9 | 10 | 25 | 35 | 30 |
27 | - | 20 | 28 | 27 | 92 | 107 | 101 | 16 | 17 | 13 | 8 | 3 | 7 | 34 | 26 | 26 |
135 | - | 26 | 27 | 26 | 109 | 104 | 106 | 16 | 19 | 19 | 10 | 4 | 7 | 28 | 34 | 31 |
675 | - | 26 | 31 | 21 | 104 | 107 | 95 | 20 | 12 | 14 | 5 | 8 | 6 | 24 | 25 | 26 |
3375 | - | 17 | 12 | 15 | 102 | 100 | 91 | 17 | 16 | 12 | 10 | 10 | 7 | 26 | 20 | 26 |
6750 | - | 1 | 1 | 1 | 26 | 17 | 25 | 4 | 4 | 8 | 2 | 4 | 1 | 17 | 19 | 19 |
5 µg MNNG | - | 742 | 713 | 792 | 612 | 621 | 658 | |||||||||
10 µg 4-Nitro-o-phenylendiamin | - | 613 | 609 | 572 | ||||||||||||
100 µg 9-Aminoacridiniumchloride monohydrate | - | 431 | 381 | 417 | ||||||||||||
5 µg 4-nitroquinoline-N-oxide | - | 250 | 264 | 134 |
Results preincubation experiment:
Dose µg/plate | metabolic activation | TA98 | TA100 | TA1535 | TA1537 | E.coli WP2 uvrA | ||||||||||
Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | ||
0 | + | 38 | 40 | 34 | 110 | 102 | 112 | 15 | 16 | 19 | 12 | 10 | 7 | 29 | 35 | 35 |
5.4 | + | 39 | 43 | 36 | 101 | 90 | 114 | 14 | 11 | 13 | 11 | 8 | 8 | 28 | 21 | 33 |
27 | + | 23 | 24 | 36 | 109 | 103 | 77 | 20 | 14 | 12 | 11 | 18 | 12 | 36 | 24 | 21 |
135 | + | 33 | 31 | 45 | 85 | 109 | 84 | 14 | 18 | 13 | 10 | 9 | 6 | 28 | 22 | 17 |
675 | + | 33 | 31 | 29 | 68 | 97 | 91 | 9 | 7 | 8 | 11 | 6 | 11 | 26 | 21 | 14 |
3375 | + | 10b | 14b | 8b | 86b | 72b | 68b | 11b | 6b | 8b | 5b | 5b | 7b | 12b | 26b | 28b |
2.5 µg 2-Aminoanthracene | + | 882 | 890 | 813 | 688 | 763 | 734 | 102 | 97 | 90 | 131 | 140 | 128 | |||
60 µg 2-Aminoanthracene | + | 255 | 211 | 270 | ||||||||||||
0 | - | 22 | 34 | 34 | 102 | 103 | 107 | 17 | 16 | 12 | 9 | 13 | 10 | 36 | 32 | 30 |
5.4 | - | 30 | 31 | 27 | 94 | 105 | 115 | 13 | 19 | 13 | 10 | 7 | 9 | 37 | 31 | 20 |
27 | - | 30 | 22 | 26 | 92 | 102 | 108 | 15 | 19 | 19 | 7 | 11 | 10 | 27 | 34 | 29 |
135 | - | 22 | 19 | 27 | 105 | 101 | 108 | 17 | 17 | 12 | 7 | 6 | 10 | 22 | 32 | 30 |
675 | - | 20 | 20 | 23 | 104 | 110 | 120 | 13 | 13 | 19 | 9 | 8 | 9 | 30 | 31 | 29 |
3375 | - | 0b | 0b | 0b | 13b | 25b | 30b | 0b | 0b | 0b | 0b | 0b | 0b | 20b | 12b | 14b |
5 µg MNNG | - | 1024 | 887 | 1149 | 820 | 917 | 754 | |||||||||
10 µg 4-Nitro-o-phenylendiamin | - | 954 | 930 | 892 | 497 | 382 | 426 | |||||||||
100 µg 9-Aminoacridiniumchloride monohydrate | - | |||||||||||||||
5 µg 4-nitroquinoline-N-oxide | - | 544 | 632 | 512 |
c: contamination
b: reduced backround growth
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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