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EC number: 276-649-0 | CAS number: 72403-66-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From September 28 to October 12, 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- not specified
- GLP compliance:
- no
- Test type:
- fixed dose procedure
- Limit test:
- yes
Test material
- Reference substance name:
- Disodium [N-(2-chlorophenyl)-2-[(2-hydroxy-5-nitrophenyl)azo]-3-oxobutyramidato(2-)][3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(2-)
- EC Number:
- 276-649-0
- EC Name:
- Disodium [N-(2-chlorophenyl)-2-[(2-hydroxy-5-nitrophenyl)azo]-3-oxobutyramidato(2-)][3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(2-)
- Cas Number:
- 72403-66-8
- Molecular formula:
- C36H21ClCrN7Na2O12S
- IUPAC Name:
- disodium [N-(2-chlorophenyl)-2-[(2-hydroxy-5-nitrophenyl)azo]-3-oxobutyramidato(2-)][3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(2-)
- Test material form:
- not specified
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Perfection Breeders, Ace Animals, Nicholas Hel f
- Age at study initiation: 9 weeks
- Weight at study initiation: from 2.2 to 3.7 kg
- Housing: housed 1/cage in suspended wire mesh cages (30" x 18" x 18")
- Diet (e.g. ad libitum): fresh Purina rabbit chow ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 1 week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 21°C
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- not specified
- Details on dermal exposure:
- TEST SITE
- Area of exposure: 200 square cm
- % coverage: ca. 10%
REMOVAL OF TEST SUBSTANCE
- Washing: warm tap water
TEST MATERIAL
- Amount(s) applied: rabbit #1 applied 6.7 cc; rabbit #2 applied 4.3 cc; rabbit #3 applied 4.1 cc; rabbit #4 applied 4.0 cc
- Constant volume or concentration used: no - Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg
- No. of animals per sex per dose:
- 2 animals per sex per dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: dermal reactions were scored at 25 hours, 7 and 14 days. 14 days for signs of toxicity, pharmacological effects and mortality. Body weights were recorded pretest and in the survivors at 7 and 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight and gross pathology
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortalities observed.
- Clinical signs:
- other: One animal had lethargy, ptosis, emaciation, diarrhea, adipsia, anorexia and few feces. See table 1.
- Gross pathology:
- At necropsy, three animals were normal and one animal had white nodules on the liver
- Other findings:
- - Other observations: 24 Hour erythema scores could not be calculated due to the color of the material. All other dermal scores at all other times were zero.
Any other information on results incl. tables
Table 1 – Toxic Signs
ANIMAL # & SEX |
HOUR |
DAY |
|||||||||||||
0 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
|
1-M |
|
|
|
|
|
|
|
|
|
|
|
|
2 |
2 |
2 |
2-M |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
3-F |
|
|
|
BQD |
BD |
BX |
BX |
BXQ |
BXQ |
BXQ |
BDQ |
BDQ |
BDQW |
BQW USX |
BQW USX |
4-F |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
CODE:
B = lethargy
D = diarrhea
Q = ptosis
S = adipsia
U = anorexia
W = emaciation
X = few feces
2 = white nasal discharge
Applicant's summary and conclusion
- Interpretation of results:
- other: CLP criteria not met
- Conclusions:
- LD50 > 2000 g/kg bw.
- Executive summary:
Method
The study was performed following a procedure similar to OECD 402.
New Zealand White rabbits, at least 8 weeks old, were equilibrated for at least one week. The animals were housed 1 per cage in suspended wire mesh cages (30" x 18" x 18"). The animal room was maintained at 20 - 21 °C and was kept clean in accordance with the standards of AAALAC.
24 hours prior to dosing, the fur was clipped from the backs of the animals. The clipped area was 200 square cm, approximately 10 %
of the body surface. Just prior to dosing, abrasions were made in one half of the rabbits. The abrasions, extending the length of the exposure site, scratched the stratum corneum but did not reach the derma or produce bleeding.
Two male and two female rabbits were dosed at 2000 mg/kg. The test material was applied once dermally to the prepared site under occlusive gauze patches. The test material was kept in contact with the skin for 24 hours, at which time the wrappings were removed. An estimate of the amount of material remaining was recorded. The exposure site was washed with warm tap water to remove excess material.
Dermal reactions were scored at 25 hours, 7 and 14 days by the Draize scoring system. The rabbits were observed daily for 14 days for signs of toxicity, pharmacological effects and mortality. Body weights were recorded pretest and in the survivors at 7 and 14 days.
Observations
All animals survived the test. At 14th day, the survivors were sacrificed. All animals were examined for gross pathology.
Results
LD50 > 2000 g/kg bw.
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