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EC number: 203-837-1 | CAS number: 111-13-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Octan-2-one
- EC Number:
- 203-837-1
- EC Name:
- Octan-2-one
- Cas Number:
- 111-13-7
- Molecular formula:
- C8H16O
- IUPAC Name:
- octan-2-one
- Test material form:
- liquid
Constituent 1
Method
- Target gene:
- Histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: G46, TA1535, TA100, C3076, TA1537, D3052, TA1538, TA98
- Species / strain / cell type:
- E. coli, other: WP2, WP2 uvrA-
- Metabolic activation:
- with and without
- Metabolic activation system:
- Mixture of rat liver enzymes
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- other: streptozotocin (without met. act.)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
10 mL of minimal agar medium are poured into a square Petri dish and allowed to solidify intno a wedge-shaped layer. 1000 µg/mL mixture of test compound in agar is prepared by adding 10 mL of minimal agar to 0.1 mL of a 100 mg/mL solution of test compound in dimethyl sulfoxide. The solution is poured into the plate. A concentration gradient (100 to 1000 µg/mL) is produced by allowing the compound in the upper wedge to diffuse into the lower layer for 2 hr at room temperature. Three additional plates with concentration ranges of 10 to 100 µg, 1 to 10 µg/mL and 0.1 to 1 µg/mL are prepared. A streaking device consisting of 10 sterile 50 µL pipets is next dipped into suspension of the 10 test strains and allowed to fill by capillary action. The pipets are then touched to the upper edge of the gradient and drawn accoss the plate.
DURATION
- Exposure duration: 48h (37 ºC)
MUTAGENIC EFFECT:
If no mutagenic events occur, a very pale streak of bacterial growth is seen along the inoculation streak. When non-lethal mutagenic events occur, discrete colonies appear in this pale background lawn. The frequency of colony appearance increases along the increasing gradient to a concentration at which maximal mutation occurs. Conversely, frequency decreases along the decreasing gradient to a concentration below which only background lawn is observable. These upper and lower concentrations are reported as the concentration range in which the compound is mutagenic under test conditions.
DETERMINATION OF CYTOTOXICITY
The concentration range over which the test compound is too toxic to permit growth of the auxotroph can be observed by the absence of growth along the application streak (i.e., no background lawn is observable). - Evaluation criteria:
- The concentration range over which chemically induced mutant colonies are present is recorded.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium, other: G46, TA1535, TA100, C3076, TA1537, D3052, TA1538, TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli, other: WP2, WP2 uvrA-
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- The susbstance was determined to be non-mutagenic.
- Executive summary:
An in-vitro gene mutation test in bacteria was performed according to a modified Ames test. S. typimurium G46, TA1535, TA100, C3076, TA1537, D3052, TA1538 and TA98, and E.coli WP2 and WP2 uvrA- strains were exposed to concentration gradients of the test item with and without metabolic activation. A plate incorporation method (in agar) was used. A concentration gradient (100 to 1000 µg/mL) was produced by allowing the compound in the upper wedge to diffuse into the lower layer for 2 hr at room temperature. Three additional plates with concentration ranges of 10 to 100 µg, 1 to 10 µg/mL and 0.1 to 1 µg/mL were prepared. The plates were incubated for 48 hr at 37 ºC. The concentration range over which chemically induced mutant colonies were recorded. The test item was determined to be non-mutagenic both with and without metabolic activation under test conditions.
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