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EC number: 609-124-5 | CAS number: 3541-81-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study performed according to OECD and GLP guidelines
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Phosphonium, (3-methyl-4-oxo-2-butenyl)triphenyl-, chloride
- EC Number:
- 609-124-5
- Cas Number:
- 3541-81-9
- Molecular formula:
- C23 H22 O P . Cl
- IUPAC Name:
- Phosphonium, (3-methyl-4-oxo-2-butenyl)triphenyl-, chloride
Constituent 1
Method
- Target gene:
- TA97a: hisD6610, TA 98 TA1537, TA1538: hisD3052; TA 100 and TA 1535 hisG46, TA102: hisG428
Species / strain
- Species / strain / cell type:
- other: TA1535, TA1537, TA1538, TA97, TA98, TA100 and TA102
- Additional strain / cell type characteristics:
- other: rfa, delta-uvrB, pKM101
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- 0, 1, 3, 10, 33, 100, 333, 500, 1000 ug/plate
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- sodium azide
- other: 2-Aminoanthracene
Results and discussion
Test results
- Species / strain:
- other: TA1535, TA1537, TA1538, TA97, TA98, TA100 and TA102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- starting at 3333 ug/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
-
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
substance is not mutagenic - Executive summary:
Wittigaldehydechlorid was tested for mutagenic activity in the Ames test. A standard plate incorporation as well
as a preincubation modification assay were performed. All seven currently used standard tester strains (TA1535, TA1537, TA1538, TA97, TA98, TA100 and TA102) were evaluated in absence and in presence of a phenobarbital/p-naphthoflavone
induced rat liver homogenate fraction (S9-mix). Responsiveness of the tester strains and activity of the S9-mix were demonstrated by using appropriate positive controls for each specific strain.
The substance was dissolved in dimethylsulfoxide (DMSO). In a preliminary toxicity experiment the compound showed bacteriotoxicity starting at 3’333 ug/plate. Therefore for the main experiments the dose range 10 to 1’000 ug/plate was selected. Due to an even stronger toxic action in the preincubation assay which became apparent at 33 ug/plate a repeat experiment in the dose
range 0.33 to 33 ug/plate was performed. No precipitation of the compound was observed up to the chosen maximum test concentration. No increase in the number of his+ revertant colonies was observed for any of the seven tester strains investigated in this study.
Therefore it can be concluded, that neither Wittigaldehydechlorid per se, nor any of its metabolites formed under the described experimental conditions is mutagenic in the Salmonella/mammalian microsome assay (Ames test).
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