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EC number: 406-080-7 | CAS number: 83016-70-0 JEFFCAT ZF-10; TEXACAT ZF-10
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-09-09 to 1997-09-12
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: EC Directive 87/302, 'Biodegradation - Activated Sludge Respiration Inhibition test'
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): JEFFCAT ZF-10
- Physica state: Clear liquid
- Lot/batch No.: 186-2-05/95 - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance, synthetic sewage, water, and inoculum
- Controls: Yes (Syntheic sewage, water, and inoculum) - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Laboratory culture: A sample of activated sludge was obtained a day before the start of the test from Oakley sewage treatment works, a sewage plant treating predominantly domestic waste. In the laboratory, the sample was maintained under aerobic conditions until required.
- Preparation of inoculum for exposure: Aliquots (25 mL) of the activated sludge were filtered through dried and preweighed Whatman's GFC filter papers which were then dried at 110 °C for at least one hour, allowed to cool in a desiccator and reweighed. The mixed liquor suspended solids (MLSS) content of the activated sludge was then calculated. Synthetic sewage (50 mL/L) was added and the mixture aerated overnight. MLSS content was determined and adjusted to 4 g/L by the addition of dechlorinated tap water.
- Pretreatment: The concentration of suspended solids in a homogenised sample was determined on the day of collection and immediately before the start of the test. - Test type:
- other: aerobic
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 18.7 - 20.0 °C (preliminary test)
18.7 - 20.4 °C (final test) - pH:
- 7.3 - 7.9 (preliminary test)
7.3 - 8.1 (final test) - Nominal and measured concentrations:
- Preliminary test : 1, 10, 100 mg/L (nominal)
Final limit test with triplicate vessel containing test substance at a nominal concentration of 100 mg/L - Details on test conditions:
- Mixtures were placed into one-litre glass beakers and aerated using a Pasteur pipette connected to a laboratory supply of oil-free compressed air for three hours. Following the exposure period, a well-mixed sample of each mixture was transferred to a biochemical oxygen demand (BOD) bottle (capacity; 270 mL) and its rate of oxygen consumption over a period of approximately ten minutes was measured using a Yellow Springs Instruments (YSI) dissolved oxygen meter, with temperature probe and self-stirring bottle probe, connected to a chart recorder. The pH and temperature of the samples were measured at the start and end of the test.
- Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol (3.0, 10.0 and 32.0 mg/L)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- In the preliminary test, the respiration rate of the sludge was decreased by 2% at a nominal concentration of 10 mg/L. In the final limit test, the respiration rates of sludges in three replicates at a nominal concentration of 100 mg/L were increased by 3, 7 and 11%. These effects are not thought to be biologically significant.
- Results with reference substance (positive control):
- Results show that the tests were valid and that the samples of activated sludge employed were sensitive to inhibition.
- Validity criteria fulfilled:
- yes
- Remarks:
- The three-hour EC50s for 3,5-dichlorophenol fulfilled the validity criterion relating to sensitivity to inhibition.
- Conclusions:
- The test substance had no significant inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the tests, including at a concentration of 100 mg/L which was the highest concentration tested.
Reference
Description of key information
The Activated sludge, respiration inhibition test of test substance was conducted according to OECD guideline 209 and GLP (Huntsman 1997).Test substance had no significant inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the tests, including at a concentration of 100 mg/L which was the highest concentration tested. IC50 (3h) was determined was greater than 100 mg a.i /L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
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