N-(4-amino-9,10-dihydro-3-methoxy-9,10-dioxo-1-anthryl)benzenesulphonamide

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

OECD Guideline 106 (Adsorption - Desorption Using a Batch Equilibrium Method)

Principles of method if other than guideline:

None

GLP compliance:

yes

Type of method:

HPLC estimation method

Test temperature:

None

Details on study design: HPLC method:

The determination was carried out using the HPLC screening method, designed to be compatible with Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001.
The test system utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.

The sample, dead time and reference standard solutions were injected in duplicate using the following HPLC parameters:
HPLC System: Agilent Technologies 1200 series
Column: Luna Cyano 5 μm 100 Å (150 x 4.6 mm id)
Column temperature: 30 ºC
Mobile phase:
Methanol: purified water (55:45 v/v)
pH of mobile phase:7.0
Flow-rate: 1.0 mL/min
Injection volume: 10 μL
UV detector wavelength: 210 nm dead time and standards & 520 nm sample

Analytical monitoring:

yes

Details on sampling:

None

Type:

Koc

Value:

2.17

Type:

log Koc

Value:

4.34

Validity criteria fulfilled:

not specified

Conclusions:

The adsorption coefficient (Koc) of the test item has been determined to be 2.17 x 10E4, log10 Koc 4.34. These values indicate that the test item will demonstrate immobility in the environment.

Executive summary:

A study was performed to determine the adsorption of FAT 36034/H according to OECD Guideline. The test item has no dissociation constants relevant to this test, therefore manipulation of the mobile phase pH was unnecessary. Testing performed at approximately neutral pH with the test item in a non-ionized form. As the slope of the calibration curve for the reference standards showed good first order correlation and as the retention times between duplicate injections for each solution were consistent, the HPLC method was considered valid for the determination of adsorption coefficient. The adsorption coefficient (Koc) of the test item has been determined to be 2.17 x 10E4, log10 Koc 4.34. These values indicate that the test item will demonstrate immobility in the environment.

Description of key information

A study was performed to determine the adsorption of FAT 36034/H according to OECD Guideline. The test item has no dissociation constants relevant to this test, therefore manipulation of the mobile phase pH was unnecessary. Testing performed at approximately neutral pH with the test item in a non-ionized form. As the slope of the calibration curve for the reference standards showed good first order correlation and as the retention times between duplicate injections for each solution were consistent, the HPLC method was considered valid for the determination of adsorption coefficient. The adsorption coefficient (Koc) of the test item has been determined to be 2.17 x E4, log10 Koc 4.34. These values indicate that the test item will demonstrate immobility in the environment.

Key value for chemical safety assessment

Koc at 20 °C:

21 700