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EC number: 201-963-1 | CAS number: 90-04-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- From 27 APR 1989 to 15 JUN 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.2 (Acute Toxicity (Inhalation))
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- o-anisidine
- EC Number:
- 201-963-1
- EC Name:
- o-anisidine
- Cas Number:
- 90-04-0
- Molecular formula:
- C7H9NO
- IUPAC Name:
- 2-methoxyaniline
- Details on test material:
- - Name of test material (as cited in study report): o-Anisidin D
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, Kastengrund, SPF-breed
- Age at study initiation: 8 to 10 weeks
- Weight at study initiation: males mean: 208 g; females mean: 197 g
- Housing: grouped (5 animals per cage) in macrolon cages (type 4) in fully airconditioned rooms
- Diet: rats diet Altromin1324 (Altromin GmbH, Lage/Lippe, Germany), ad libitum
- Water: tap water, ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22+/-2
- Humidity (%): 50+/-20
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus:cylindrical plastic cage
- Exposure chamber volume: 60 L
- Method of holding animals in test chamber: each animal was in plastic tube
- Source and rate of air: laminar air stream of 800L/h at 4 bar from above
- Method of particle size determination: "Anderson-Kaskadenimpactor Mark III" of Anderson Samples Inc., Atlanta, USA
- Treatment of exhaust air: exhaust device at the basement of the exposure chamber in line with a diverse system of filters
- Temperature, humidity, pressure in air chamber: air control system of Hartman&Braun
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution:
at dose 2.17 mg/l: <0.6 µm to 7 µm
at dose 3.87mg/l: <0.6 µm to 4.8 µm
- MMAD (Mass median aerodynamic diameter):
at dose 2.17 mg/l: 1.71
at dose 3.87mg/l: 1.58 - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- gas chromatography
- Duration of exposure:
- 4 h
- Concentrations:
- 2.17 and 3.87 mg/l (highest technically feasible exposure concentration)
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: twice daily
- Frequency of weighing: weekly
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 3.87 mg/L air
- Exp. duration:
- 4 h
- Remarks on result:
- other: higest technically feasible exposure concentration, no animals died during the 14 days observation period
- Mortality:
- - no deaths occured
- Clinical signs:
- other: - impairment of movement, respiration and reflexes were observed, as well as blood nasal discharge and cyanosis - all animals were free of clinical signs from day 8 after application
- Body weight:
- - body weight development in two female animals was impaired during the first week of the test. Nevertheless, they gained weight within the second week of the experiment.
- in all other animals body weight development was not impaired - Gross pathology:
- - no macrosopically visible changes were found
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- Exposure of male and female Wistar rats to the highest technically feasible concentration of 3.87 mg test item o-Anisidin D per L air for 4 hours did not result in the death of the animals during a 14 days observation period, resulting in a LC50 value of > 3.87mg/L air. The test results are adequate to fulfil the endpoint requirements.
- Executive summary:
Acute inhalation toxicity of the test item o-Anisidin D has been investigated in male and female Wistar rats according to a standard acute method (OECD TG 403). They were exposed to 2.17 or 3.87 mg test substance per liter air for 4 h (maximal applicable dose). All animals survived the 14 days observation period. No macroscopic visible changes were observed at necropsy at the end of the observation period, resulting in a LC50 value of > 3.87 mg/L for the inhalation of aerosol.
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