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EC number: 258-004-5 | CAS number: 52556-42-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- , difference in range-finding test slightly above 2 °C (+0.2 °C). This slight deviation did not affect the result of the range-finding study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
- Sampling method: 3 mL samples were taken at t=0 and t=96
- Sample storage conditions before analysis: storage in a freezer (stability under conditions was determined in previous project) - Vehicle:
- no
- Details on test solutions:
- Preparation of test solution started with the highest test concentration of nominal 400 mg/L. This concentration corresponded to 100 mg a.i./L. No special treatment other than a short period of magnetic stirring (15 minutes) was applied to ensure complete dissolution in the test medium. The pH of this solution was above 9.0 (9.4 and 9.5, respectively in range-finding and final test) and as a consequence adjusted to 7.8 using 1M HCl (Merck, Darmstadt, Germany). The lower test concentrations were prepared by subsequent dilutions in test medium. The final test solutions were all clear and colourless.
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: carp
- Strain: Linnaeus 1758
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen, University and Research Centre, NL
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): range-finding: 2.4 ± 0.2 cm, final test: 2.6 ± 0.2 cm
- Weight at study initiation (mean and range, SD): range-finding: 0.43 ± 0.09 g, final test: 0.53 ± 0.10 g
- Method of breeding:
- Feeding during test
- Food type: -
- Amount: -
- Frequency: no feeding from 24 hours prior to the test and during the total test period
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): adjusted ISO medium
- Type and amount of food: pelleted fish food (Nutra 3.0 T, TROUW nutrition, Putten, NL)
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no information availble - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 180 mg CaCO3
- Test temperature:
- control: 21.8-22.7 °C
100 mg/L: 21.7-23.1 °C - pH:
- control: 7.5-7.9
100 mg a.i./L: 7.4-7.9 - Dissolved oxygen:
- control: 8.5-9.2 mg/L (6.2 mg/L after 29 hours)
100 mg/L: 8.4-9.2 mg/L (5.5 mg/L after 29 hours)
aeration was introduced after 29 hours, as the oxygen concentration tended to drop below the optimum level for testing with carp - Nominal and measured concentrations:
- 0.1, 1.0, 10 and 100 mg a.i./L
Analysis of the samples taken at the start and the end of the test showed that measured concentrations were in agreement with nominal (100 %). - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 litres
- Material, size, headspace, fill volume: all-glass, 7.7 L test solution
- Aeration: introduced after 29 hours
- No. of organisms per vessel: 3 fishes/concentration (range-finding test), 7 fishes/concentration (limit test)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):1
- Biomass loading rate: 0.48 fish/litre
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: adjusted ISO medium
OTHER TEST CONDITIONS
- Adjustment of pH: only before testing period
- Photoperiod: 16 h daily
- Light intensity: o information available
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : lethality (24, 48, 72, 96 h)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 for range-finding test
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 0.1, 1.0, 10 mg/L - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol (PCP)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- behaviour
- Details on results:
- - Behavioural abnormalities: no clinical effects were observed
- Results with reference substance (positive control):
- - Results with reference substance valid? yes, findings of LC50(96h) were within the range of historical data
- Mortality: yes
- LC50: 0.15 mg/L (95% c.i.: 0.10-0.22 mg/L) - Reported statistics and error estimates:
- no statistics reported due to absence of effects in test
- Sublethal observations / clinical signs:
Table 1. Incidence of mortality and total mortality during range-finding test
Concentration HAPS (mg a.i./L)
Initial number of fish
Cumulative mortality
Total mortality (%)
4 h
24 h
48 h
72 h
96 h
0.1
3
0
0
0
0
0
0
1.0
3
0
0
0
0
0
0
10
3
0
0
0
0
0
0
100
3
0
0
0
0
0
0
Table 2. Incidence of mortality and total mortality during limit test
Concentration HAPS (mg a.i./L)
Initial number of fish
Cumulative mortality
Total mortality (%)
3¼h
24 h
48 h
72 h
96 h
Control
7
0
0
0
0
0
0
100
7
0
0
0
0
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- No visible effects observed in carp under test conditions up to highest test concentrations.
- Executive summary:
Based on the results of a preceding range-finding test a final test was performed as a limit test. Seven carp per test group were exposed to a control and a HAPS concentration of 100 mg a.i./L. The total test period was 96 hours and a static test system was applied. Samples for analytical confirmation of actual exposure concentrations were taken at the start and the end of the test period.
Analysis of the samples taken at the start and the end of the limit test showed that measured concentrations were in agreement with nominal (100 %). The study met the acceptability criteria prescribed by the protocol and was considered valid.
HAPS induced no visible or lethal effects in carp at or below 100 mg a.i./L (NOEC). The 96h-LC50 exceeded 100 mg a.i./L (corrected for purity and based on analytically confirmed nominal concentrations).
Reference
Description of key information
In absence of visible effects in available study with fish key value is set to the maximal concentration used in the study for the further assessment.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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