2-(octylthio)ethanol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: According to NICNAS source listed below, the study was conducted with methods similar to OECD guidelines.

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

Prevention, Pesticides and Toxic Substances Reregistration Eligibility Decision (RED) Hydroxyethyl Octyl Sulfide

Author:

U.S. EPA

Year:

1995

Bibliographic source:

EPA 738-R-96-001

Reference 2

Reference Type:

secondary source

Title:

Full Public Report Ethyl Octyl Sulphide

Author:

National Industrial Chemicals Notification and Assessment Scheme

Year:

1998

Bibliographic source:

NA/487

Reference 3

Reference Type:

study report

Title:

Unnamed

Year:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Ethanol, 2-(octylthio)-

IUPAC Name:

Ethanol, 2-(octylthio)-

Constituent 2

Reference substance name:

2-(octylthio)ethanol

EC Number:

222-598-4

EC Name:

2-(octylthio)ethanol

Cas Number:

3547-33-9

Molecular formula:

C10H22OS

IUPAC Name:

2-(octylsulfanyl)ethan-1-ol

Constituent 3

Reference substance name:

Hydroxyethyl octyl sulfide

IUPAC Name:

Hydroxyethyl octyl sulfide

Details on test material:

- Name of test material (as cited in study report): Hydroxyethyl octyl sulfide
- Substance type: Active
- Physical state: Liquid
- Analytical purity: 96.9 %

Method

Target gene:

Histidine locus

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA98, TA100, TA1535 and TA1538

Metabolic activation:

with and without

Metabolic activation system:

Rat liver S9

Test concentrations with justification for top dose:

0, 3, 10, 33 and 333 ug/plate

Vehicle / solvent:

No data

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

No data

Evaluation criteria:

No data

Statistics:

No data

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA98, TA100, TA1535 and TA1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Additional information on results:

ADDITIONAL INFORMATION ON CYTOTOXICITY: At doses above 100 ug/plate, in the absence of activation, toxicity was moderate to extreme.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

NICNAS also lists TA1537 as having been tested.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Based on the results of the study, the test substance failed to induce a mutagenic response in the Ames assay either in the presence or absence of metabolic activation up to a concentration level of 333 ug/plate.

Executive summary:

In a bacterial reverse mutation assay,Salmonella typhimurium, conducted with methods similar to OECD 471 guidelines, S. typhimurium strains TA98, TA100, TA1535 and TA1538 were treated with ethanol, 2-(octylthio) (96.9%) at concentrations of 0, 3, 10, 33 and 333 µg/plate in the presence and absence of rat liver S9 activation. No induced mutations were observed in the indicator strains in either the presence or absence of metabolic activation. At doses above 100 µg/plate, in the absence of activation, toxicity was moderate to extreme.