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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP, guideline study, published in peer reviewed literature, limitations in reporting but otherwise acceptable for assessment.

Data source

Reference
Reference Type:
publication
Title:
Brine shrimp bioassay and seawater BOD of petrochemicals
Author:
Price, Waggy and Conway
Year:
1974
Bibliographic source:
Water Pollution Control Federation 46(1), 63-77

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: APHA method n° 219 (1971)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Toluene
EC Number:
203-625-9
EC Name:
Toluene
Cas Number:
108-88-3
Molecular formula:
C7H8
IUPAC Name:
toluene

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic, adapted
Details on inoculum:
3ml settled domestic waste water, filtered through glass wool. Adapted.
Duration of test (contact time):
20 d
Initial test substance concentrationopen allclose all
Initial conc.:
3 mg/L
Based on:
test mat.
Initial conc.:
7 mg/L
Based on:
test mat.
Initial conc.:
10 mg/L
Based on:
test mat.
Initial conc.:
> 3 - < 30 mg/L
Based on:
other: oxygen demand
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Tested in duplicate. When the dissolved oxygen dropped below 4mg/l the contents were reaerated. The bottles were opened for sampling about 5 times during the 20 day test.

Results and discussion

% Degradationopen allclose all
Parameter:
other: BOD/THOD
Value:
73
Sampling time:
5 d
Parameter:
other: BOD/THOD
Value:
74
Sampling time:
10 d
Parameter:
other: BOD/THOD
Value:
80
Sampling time:
15 d
Parameter:
other: BOD/THOD
Value:
86
Sampling time:
20 d

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable
Conclusions:
Toluene biodegrades rapidly under the conditions of this test.