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EC number: 410-190-0 | CAS number: 132983-41-6 MCP 968
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented study report similar or equivalent to OECD 474. GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- A mixture of isomers of: mono-(2-tetradecyl)naphthalenes; di-(2-tetradecyl)naphthalenes; tri-(2-tetradecyl)naphthalenes
- EC Number:
- 410-190-0
- EC Name:
- A mixture of isomers of: mono-(2-tetradecyl)naphthalenes; di-(2-tetradecyl)naphthalenes; tri-(2-tetradecyl)naphthalenes
- Cas Number:
- 132983-41-6
- Molecular formula:
- Can vary from C24H36 (mono rxn product) to C52H92 (tri rxn product)
- IUPAC Name:
- 2,3,6-tritetradecylnaphthalene; 2,3-ditetradecylnaphthalene; 2-tetradecylnaphthalene
- Details on test material:
- - Name of test material (as cited in study report): MCP 968
Constituent 1
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- male/female
Administration / exposure
- Route of administration:
- dermal
- Vehicle:
- neat
- Details on exposure:
- The test material was applied evenly over the back of the animal begining at the spine and scapula working posteriorly and laterally.
- Duration of treatment / exposure:
- animals were dosed daily, five days per week for four weeks.
- Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 125, 500, 2000 mg/kg/day
Basis:
other: dermal application
- No. of animals per sex per dose:
- 20 (10 males and 10 femals per dose)
- Control animals:
- yes, concurrent no treatment
Examinations
- Tissues and cell types examined:
- Femurs were taken from five animals per sex per dose group. Bone marrow was removed from the femurs and dissaggregated in heat inactivated fetal bovine serum containing 25 mM EDTA. A nearly pure erythrocyte fraction was then obtained by passing the bone marrow cell suspension through a cellulose column using a procedure based on the method developed by Romagna.
- Details of tissue and slide preparation:
- The resulting erythrocyte fraction was collected by centrifugation, and the cells were resuspended in a small volume of fetal bovine serum. Two bone marrow slides were made for each animal. Slides were randomized by a computer-generated random numbers table. One thousand PCEs and 1000 NCEs were scored to determine the percentage of micronucleated erythrocytes. One thousand erythrocytes were scored to determine the ratio of PCEs to NCEs.Slides were dried on a slide warmer at 57 °C and fixed in absolute methanol for fifteen minutes. After the slides had dried, the slides were placed in acridine orange (0.125 mg/ml in Giordano's buffer, pH 6.4-6.5) solution for 7 minutes, rinsed in Giordano's buffer, washed in Giordano's buffer for an additional 10 minutes, and finally rinsed in fresh Giordano's buffer. Fluorescent microscopy was used to evaluate the erythrocytes.
- Evaluation criteria:
- To determine if the test chemical caused cell toxicity in the bone marrow, PCEs and NCEs were counted until 1000 total erythrocytes were tallied for each animal, and the ratios of PCEs to NCEs was calculated. A smaller ratio for the test substance-treated animals compared to the controls would indicate that the test substance had induced toxicity in the bone marrow. Toxicity can reduce the sensitivity of the assay to detect the induction of micronuclei by preventing the formation of new erythrocytes. However, the presence of toxicity in bone marrow would indicate that the test substance had reached the assay tissue.
- Statistics:
- ANOVA and GLM models were applied to the data. The statistical analyses compared test values to negative control data; a significant increase in micronuclei is an indication of clastogenic activity by the test agent.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- not applicable
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
The in vivo micronucleus assay of the test material was negative. This finding does warrant the classification of the test material as a genotoxin under the new Regulation (EC) 1272/2008 on classification, labeling, and packaging of substances and mixtures (CLP) or under the Directive 67/518/EEC for dangerous substances and Directive 1999/45/EC for preparations. - Executive summary:
The micronucleus assay was used to determine if the test material caused a significant increase in the number of micronucleated red blood cells from bone marrow of rats treated dermally over thirteen weeks, five days a week at doses of 125, 500, and 2000 mg/kg. The test material was not cytotoxic to red blood cell formation nor did it induce any statistically significant increase in the formation of micronucleated PCEs or NCEs in the bone marrow of dermally-treated rats. The test material was not considered to a genotoxin or clastogen under the conditions of the test. This finding does not warrant the classification of the test material as a genotoxin under the new Regulation (EC) 1272/2008 on classification, labeling, and packaging of substances and mixtures (CLP) or under the Directive 67/518/EEC for dangerous substances and Directive 1999/45/EC for preparations.
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