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EC number: 225-060-7 | CAS number: 4635-87-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 03 SEPTEMBER 2008 to 13 JANUARY 2009
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- This study test the ready biodegradability of the test substance according to standard guidelines (OECD 310). Since the test substance is suspected to be volatile this precaution is welcome. The report is fully detailed and despite it is not GLP, results presented are highly reliable. Therefore this data can be considered of high reliability with the only restriction it is not GLP.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- Deviations:
- no
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge was collected from the aeration tank of a full-scale wastewater treatment plant which treats predominantly domestic sewage (ETE Jundiai-SP/Brazil).
- Laboratory culture: no
- Method of cultivation:no
- Storage conditions:no data
- Storage length: none
- Preparation of inoculum for exposure: The activated sludge was treated by allowing to settle three times for 30 minutes. For each settling the supernatant was rejected and the solids were redispersed in the mineral medium. In order to lower carbon organic content and to reduce the blank CO2 evolution, the activated sludge was aerated overnight. The concentration of the treated activated sludge was adjusted to 1000 mg/L dry solids. In order to achieve a final concentration of 4 mg/L activated sludge dry solids, the treated activated sludge was diluted with the mineral medium.
- Pretreatment: no
- Concentration of sludge: 4 mg/L
- Initial cell/biomass concentration: no data
- Water filtered: no data
- Type and size of filter used, if any: none - Duration of test (contact time):
- 28 d
- Initial conc.:
- 19.91 other: mgC/L
- Based on:
- ThIC
- Parameter followed for biodegradation estimation:
- inorg. C analysis
- Details on study design:
- TEST CONDITIONS
- Composition of medium: KH2PO4 (85 mg/L), K2HPO4 (217.5 mg/L), Na2HPO4, 2H20 (334 mg/L), NH4Cl (5 mg/L), CaCl2, 2H2O (36.4 mg/L), MgSO4, 7H20 (22.5 mg/L), FeCl3, 6H2O (0.25 mg/L)
- Additional substrate: none
- Solubilising agent (type and concentration if used): none
- Test temperature: 22 +- 2°C
- pH: 7.62
- pH adjusted: no
- CEC (meq/100 g): no data
- Aeration of dilution water: no data
- Suspended solids concentration: 4 mg/L
- Continuous darkness: incubation in the dark
TEST SYSTEM
- Number of culture flasks/concentration: 15 bottles for blank control, test and reference treatments. 4 bottles for inhibition controls.
- Method used to create aerobic conditions: shaking in a rotary apparatus at 160-10 rpm
- Test performed in closed vessels due to significant volatility of test substance: 120mL-bottles sealed with butyl rubber stoppers and aluminium crimp seals.
SAMPLING
- Sampling frequency: on day 2, 5, 7, 14, 21, and 28
- Sampling method: see "analytical method"
- Sterility check if applicable: no abiotic control
- Sample storage before analysis: no data
CONTROL AND BLANK SYSTEM
- Inoculum blank: 80 mL of inoculated medium
- Abiotic sterile control: none
- Inhibition control: 80 mL of inoculated medium containing test substance (19.91 mg/L TOC) and reference substance (20 mg/L TOC)
- Other: reference bottles (80 mL of inoculated medium containing the reference substance at 20 mg/L TOC)
STATISTICAL METHODS:
no statistics were performed - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- none
- Test performance:
- met all validity criteria
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 48.81
- Sampling time:
- 5 d
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 68.12
- Sampling time:
- 7 d
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 69.31
- Sampling time:
- 14 d
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 81.3
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 83.94
- Sampling time:
- 28 d
- Details on results:
- On day 2, 1.07% of degradation was measured.
The ihibition control showed the test substance was not toxic to the inoculum with 90.62 % of biodegradation after 28 days. - Results with reference substance:
- Sodium benzoate was degraded at 74.93% after 2 days and 97.06% after 28 days of test. Furthermore, this reference substance passed the OECD "10-day window" criterion.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance degraded at 83.94% after 28 days based on inorganic carbon analysis. The 10-day window started on day 5, and the pass level of 60 % degradation was attained during these 10 days. Thus the test substance is considered as readily biodegradable.
- Executive summary:
This study tests the ready biodegradability of the test substance according to standard guidelines (OECD 310). The report is fully detailed and despite it is not GLP, results presented are highly reliable.
Biodegradation of the test substance was measured by inorganic carbon analysis on day 2, 5, 7, 14, 21, and 28 in duplicate but on day 28 where five replicates (bottles) were measured. The inoculum used was sampled from a WWTP receiving domestic sewage and was aged carefully prior to testing. Suspended matter concentration in the inoculum was adjusted to 4 mg/L. Blank, test, reference, and toxicity bottles were filled in with mineral medium plus inoculum, test substance, sodium benzoate or both respectively. Each 120 mL-bottles were filled up to 80 mL and sealed with butyl rubber cap and aluminum crimp seals. Concentration of the test substance was 19.91 mg/L TOC.
The 10d-window started before day 5 (48.81%) and 69.31% degradation was calculated on day 14. The test was terminated on day 28 drawing 83.94% of degradation of the test substance. All validity criteria were met.
Therefore the test substance is considered to be readily biodegradable meeting the 10d-window.
Reference
Description of key information
Based on reliable data, the test substance is readily biodegradable and meets the 10-day window.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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