Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 600-033-6 | CAS number: 1001416-18-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
- Endpoint:
- fish short-term toxicity test on embryo and sac-fry stages
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, no deviations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1400 (Fish Early-life Stage Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-Propanone, 2-hydroxy-2-methyl-, 1-(4-C10-13-alkylphenyl) derivs.
- Cas Number:
- 1001416-18-7
- Molecular formula:
- Not applicable - UVCB
- IUPAC Name:
- 1-Propanone, 2-hydroxy-2-methyl-, 1-(4-C10-13-alkylphenyl) derivs.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 4.1, 8.1, 16, 33, 65 µg/L (nominal conc.)
- Sampling method: Samples from test solutions were removed from the approximate midpoint of each aquarium using a pipette.
- Sample storage conditions before analysis: No data
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A 2.6 mg/mL diluter stock solution was prepared prior to test initiation and as needed throughout the definitive exposure by placing, for example, approximately 0.1302 g of test substance in a 50-mL volumetric flask and bringing it to volume with dimethylformamide, DMF (CAS No. 68-12-2). The resultant solution was observed to be clear and colorless following preparation.
A 520 μL/mL solvent stock solution was prepared by placing 519 mL of DMF in a 1000-mL volumetric flask and bringing it to volume with deionized water. The resultant solution was observed to be clear and colorless following preparation.
Prior to test initiation, a Harvard Apparatus syringe pump in conjunction with a 50-mL Glenco® gas-tight syringe was calibrated to deliver 0.0485 mL/cycle of the diluter stock solution (2.6 mg/mL) into the diluter's chemical mixing chamber, which received 1.94 L of dilution water per cycle. The solution contained in the mixing chamber constituted the highest nominal test concentration (65 μg/L) and was subsequently proportionally diluted (50%) to provide the remaining nominal exposure concentrations (33, 16, 8.1 and 4.1 μg/L).
The concentration of DMF in the solution in the mixing chamber and the high test concentration constituted the highest DMF concentration (0.025 mL/L). A Fluid Metering Inc., pump was calibrated to deliver 0.244 mL/cycle of the solvent stock solution (520 μL/mL) to 5.065 L of dilution water per cycle which was subsequently delivered to the solvent control and treatment vessels. The DMF concentration in the solvent control and the treatment levels was 0.025 mL/L), which was equal to that of the high test concentration. A set of control vessels was also established which contained the same dilution water and was maintained under the same conditions as the treatment level vessels, but contained no solvent or test substance.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Dimethylformamide
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 0.025 mL/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The stock solution was clear and colourless
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Source: Fathead minnow eggs were obtained from brood stock maintained at Springborn Smithers. The brood stock originated from the U.S. Environmental Research Laboratory, Duluth, Minnesota.
METHOD FOR PREPARATION AND COLLECTION OF FERTILIZED EGGS
- Numbers of parental fish (i.e. of females used to provide required number of eggs): No information
- Method of collection of fertilised eggs: No information
- Subsequent handling of eggs: No information
POST-HATCH FEEDING
- Start date: When hatch was designated as being complete
- Type/source of feed: live brine shrimp nauplii (Artemia salina)
- Amount given: ad libitum
- Frequency of feeding: Three times per day
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 32 d
- Post exposure observation period:
- No post exposure observation period
Test conditions
- Hardness:
- Total hardness as CaCO3: 68 to 88 mg/L
- Test temperature:
- 25 - 27 °C
- pH:
- 6.4 - 7.2
- Dissolved oxygen:
- Control: 6.8-8.4 mg/L
Solvent control: 4.9-7.9 mg/L
Test concentrations (4.1, 8.1 33, 65 µg/L): 5.2-8.1 mg/L; 16 µg/L: 2.2.-7.8 mg/L - Salinity:
- No information
- Nominal and measured concentrations:
- Nominal test concentrations: 4.1, 8.1, 16, 33, 65 µg/L
Measured test concentrations: 3.1, 6.2, 15, 31, 59 µg/L - Details on test conditions:
- TEST SYSTEM
- Emybro cups: Embryo incubation cups were round glass jars (5 cm O.D., 8 cm high) with Nitex® screen bottoms with 475-μm screen openings
- Test vessel: aquaria
- Type (delete if not applicable): open / closed
- Material, size, fill volume: glass, 30x15x20 cm, 250 ml
- Aeration: no information
- Type of flow-through: intermittent-flow proportional diluter
- Renewal rate of test solution (frequency/flow rate): 90 % replacement of test solution within ca. 7 hours
- No. of fertilized eggs/embryos per vessel: 30
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: max. 0.066 g/L of flowing test solution per day or 0.50 g/L at any time in any replicate exposure aquarium based on control organism wet weight at test termination.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: on site well water, aerated prior to use
- Total organic carbon: no information
- Particulate matter: no information
- Metals, pesticides:Representative samples of the dilution water source were analyzed periodically for the presence of pesticides, PCBs and toxic metals by GeoLabs, Inc., Braintree, Massachusetts. None of these compounds were detected at concentrations considered toxic in any of the water samples analyzed, in agreement with ASTM (2005) standard practices.
- Chlorine: no information
- Alkalinity: 18-24 mg/L as CaCO3
- Conductivity: 450 to 470 μmhos/cm
- Salinity: no information
- Culture medium different from test medium: no
- Intervals of water quality measurement: weekly
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours of light, 8 hours of darkness
- Light intensity: 64-89 footcandles (680-960 lux)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :embryo hatching success, percent of embryos that produced normal larvae (at completion of hatch),
survival, growth and weight of larvae (at test termination(28 days post hatch))
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations: 0.063, 0.13, 0.25, 0.5, 1 mg/L
- Results used to determine the conditions for the definitive study: yes
POST-HATCH DETAILS
- Begin of post-hatch period: on day of hatch (test day 4)
- No. of hatched eggs (alevins)/treatment released to the test chamber: 15 per replicate (total of 60 organisms
- Release of alevins from incubation cups to test chamber on day no.: test day 4
FERTILIZATION SUCCESS STUDY
- Number of eggs used: no information
- Removal of eggs to check the embryonic development on day no.: no information - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 59 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: hatching success, percent normal larvae at completion of hatch, survival, total length and dry weight of fathead minnow larvae
- Duration:
- 32 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 59 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: hatching success, percent normal larvae at completion of hatch, survival, total length and dry weight of fathead minnow larvae
- Details on results:
- - Larval survival::
Control:93 %
Solvent control: 88 %
Pooled control: 92 %
3.1 µg/L: 87 %
6.2 µg/L: 97 %
15 µg/L: 87 %
31 µg/L: 90 %
59 µg/L: 90 %
- Days to hatch or time to release of young: 4
- Hatching success:
Control: 93 %
Solvent control: 95 %
Pooled control: 94 %
3.1 µg/L: 90 %
6.2 µg/L: 93 %
15 µg/L: 85 %
31 µg/L: 87 %
59 µg/L: 93 %
- Normal larvae at hatch:
Control: 98 %
Solvent control: 99 %
Pooled control: 99 %
3.1 µg/L: 100 %
6.2 µg/L: 100 %
15 µg/L: 87 %
31 µg/L: 100 %
59 µg/L: 98 %
- Length of larvae:
Control: 27.7 mm
Solvent control: 28.2 mm
Pooled control: 28.0 mm
3.1 µg/L: 28.0 mm
6.2 µg/L: 27.7 mm
15 µg/L: 27.8 mm
31 µg/L: 27.6 mm
59 µg/L: 28.5 mm
- Dry weight of larvae:
Control: 0.0497 g
Solvent control: 0.0499 g
Pooled control: 0.0498 g
3.1 µg/L: 0.0509 g
6.2 µg/L: 0.0485 g
15 µg/L: 0.0506 g
31 µg/L: 0.0482 g
59 µg/L: 0.0532 g - Results with reference substance (positive control):
- Not applicable
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Exposure of fertilized eggs of pimephales promelas to the test substance at concentrations up to 59 µg/L for 32 days did not affect the embryo hatching success, development of normal larvae, larval survival, length and dry weight of larvae.
- Executive summary:
In an early life satage study according to OECD Guideline 210 and OPPTS Draft Guideline 850.1400 fertilized eggs of Fathead Minnow (Pimephales promelas) were exposed to the test substance at nominal concentrations of 4.1, 8.1, 16, 33 and 65 µg/L (measured concentrations: 4.1, 8.1, 16, 33 and 59 µg/L) under flow through conditions. Dimethylformamide was used as a vehicle in a concentration of 0.025 mL/L. After completion of hatch at test day 4 the larvae were exposed for another 28 days to the test substance. The embryo hatching success was assessed and at termination of the test the development of normal larvae, larval survival, length and dry weight of larvae were assessed. No adverse effects were observed. Therefore a NOEC of 59 µg/L and a LOEC of > 59 µg/L were derived.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.