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EC number: 275-833-8 | CAS number: 71675-87-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2 December 2014 - 30 December 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Name of test material (as cited in study report): Acilian
- batch No.of test material:GS00012
- Expiration date of the lot/batch:February 28, 2015
- Purity test date:March 13, 2014
- Purity: 98.0 – 101.5% (nominal)
100.9% (analytical), according to certificate of analysis
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:At room temperature (20 ± 5 °C), in the dark and dry conditions
- Stability under test conditions: yes - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge :Microorganisms from a domestic waste water treatment plant was supplied by the sewage plant Rossdorf, Germany
- Preparation of inoculum for exposure: The aerobic activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and centrifuged again. This procedure was done three times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water and aerated overnight. This suspension was used for the experiment.
- Concentration of sludge: Stock suspension of 3.5 g/L on dry matter base (final sludge concentration in test flasks: 28.7 mg sludge/L) - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- ca. 102.7 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Fifty mL of stock solution a) and 5 mL of the stock solutions b) to d) were combined and filled up to a final volume of 5000 mL with deionised water.
Stock solutions:
a) 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4 x 2 H2O, 0.5 g NH4Cl filled up with deionised water to 1000 mL volume; The pH-value was 7.4.
b) 11.25 g MgSO4 x 7 H2O filled up with deionised water to 500 mL volume
c) 18.2 g CaCl2 x 2 H2O filled up with deionised water to 500 mL volume
d) 0.125 g FeCl3 x 6 H2O filled up with deionised water to 500 mL volume
In order to avoid precipitation of iron hydroxide in the stock solution d) after storage and before use, one drop of concentrated HCl per litre was added.
- Test temperature: 22°C ± 1°C.
- pH: 7.5 to 7.6 (measured at the start of the test)
7.3 to 7.9 (measured at the end of the test)
- pH adjusted: NO
- Suspended solids concentration: 28.7 mg sludge/L
- Continuous darkness: yes
- Water:deionised water
TEST SYSTEM
- Number of culture flasks/concentration: 2 flask - Test item: flask 1and 2 (Total - 7 flasks, see part CONTROL AND BLANK SYSTEM below)
- Method used to create aerobic conditions: Stirring
- Measuring equipment: automated Software AMR Wincontrol - to measure Temperature
pH-electrode WTW pH 340i. - to measure pH
Measurement of oxygen:(BSB/BODSensor - System, Aqualytic Dortmund, Germany)
- Test performed in closed vessels due to significant volatility of test substance:yes
- Test performed in open system: No
- Details of trap for CO2 :It was absorbed in an aqueous solution (45%) of potassium hydroxide.
- Measuremnt of oxygen: The consumption of oxygen was determined by measuring the change of pressure in the flasks. The change of pressure in the test flasks was measured by means of a manometric method each day.
SAMPLING
- Sampling frequency: every day, 28 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes - FLASK 3 and 4
- Abiotic sterile control:Yes - FLASK 6 (Poisoned with 5 mL HgCl2 solution in test water (stock solution of 1 g/L))
- Toxicity control:Yes - FLASK 7
- Procedure control:Yes - FLASK 5 - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- SLBK0666V, 99.9%
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 0
- Sampling time:
- 28 d
- Details on results:
- The oxygen demand of the inoculum control (medium and inoculum) was 30 mg O2/L, of the test item was 25 mg O2/L and for toxicity control was 185 mg O2/L at the day 28.The difference of duplicate values for the degradation of the test item at the plateau, at the end of the test and at the end of the 10-day window was less than 20%. The difference of duplicate values at day 28 differed by 0%. At the end of the test at day 28, the degradation of test item was 0% and for the toxicity control the biodegradation was 49% after 28 days of incubation. Test item did not reach 60% of biodegradation during the incubation period of 28 days and therefore the 10 window could not be determined.
In the toxicity test, containing both the test substance and a reference compound more than 25% biodegradation (based on total ThOD) occurred within 14 days, therefore the test substance can be assumed to be not inhibitory.
ABIOTIC CONTROL: as the oxygen demand was 0 mg/L during the test, there was no need to use correction of degradation of the test item and toxicity control - Results with reference substance:
- The reference item sodium benzoate was degraded to more than 60% after 3 days of incubation. It was sufficiently degraded to 81% after 14 days and to 82% after 28 days of incubation. The percentage biodegradation of the reference item confirms the suitability of the used aerobic activated sludge inoculum.
- Validity criteria fulfilled:
- yes
- Remarks:
- Differences of replicates < 20%.The reference item reached the pass level >=60%on day 3 .The oxygen uptake of the inoculum blank was 30 mg O2/l in 28 days.The pH values of flasks was inside the range 6.0 - 8.5,Oxygen consumption by the test item is < 60%
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test substance is considered to be not readily biodegradable under aerobic aqueous conditions.
- Executive summary:
The study of the 28 day ready aerobic biodegradability of test item, was carried out according GLP principles and OECD 301F/EC C.4 – D manometric respirometry methods, at the temperature 22 °C. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. The test item contains nitrogen; therefore the evaluation of biodegradation has to be based ThODNH4 and ThONO3. The 102.7 mg/L of test item was inoculated with activated sludge (28.7 mg/L SS) and incubated under aerobic conditions in a closed respirometer flask at the constant temperature. A reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. A blank test, toxicity test and Abiotic sterile control test were run in parallel. The reference item was sufficiently degraded to 81% after 14 days and to 82% after 28 days of incubation. In the toxicity test, containing both the test item and a reference item, on the 14th test day the biodegradation (based on ThODNH4 ) attained 45 % and after 28 days of incubtaion 49% ( ThODNH4 ) - test item can be asumed to be not inhibitory.
At the 28th day of the test the measured aerobic biodegradation of the test item equals 0 %. According to OECD criteria the pass level for ready biodegradability for respirometric methods are 60% of ThOD.
Thus the test item cannot be deemed to be readily biodegradable.
Reference
Nitrogen is part of the molecular structure of the test item; therefore nitrification (ThODNO3) has to be considered for the evaluation of results.
Table No.2: Cumulative Biochemical Oxygen Demand (mg O2/L) in Test Flasks during the Test
Period of 28 Days
Time days |
FLASK No. |
||||||
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
1 |
0 |
0 |
0 |
0 |
60 |
0 |
60 |
2 |
0 |
0 |
0 |
0 |
85 |
0 |
85 |
3 |
0 |
0 |
0 |
0 |
110 |
0 |
110 |
4 |
5 |
0 |
10 |
10 |
120 |
0 |
120 |
5 |
5 |
0 |
10 |
10 |
125 |
0 |
130 |
6 |
5 |
5 |
10 |
10 |
135 |
0 |
135 |
7 |
5 |
5 |
10 |
10 |
140 |
0 |
140 |
8 |
10 |
10 |
15 |
15 |
145 |
0 |
145 |
9 |
10 |
10 |
15 |
15 |
145 |
0 |
145 |
10 |
10 |
10 |
15 |
15 |
150 |
0 |
150 |
11 |
10 |
10 |
15 |
15 |
155 |
0 |
155 |
12 |
10 |
10 |
15 |
15 |
155 |
0 |
155 |
13 |
15 |
15 |
20 |
15 |
155 |
0 |
160 |
14 |
15 |
15 |
20 |
15 |
155 |
0 |
160 |
15 |
15 |
15 |
20 |
15 |
160 |
0 |
165 |
16 |
20 |
20 |
20 |
20 |
160 |
0 |
170 |
17 |
20 |
20 |
25 |
20 |
160 |
0 |
170 |
18 |
20 |
20 |
25 |
20 |
160 |
0 |
170 |
19 |
20 |
20 |
25 |
20 |
160 |
0 |
175 |
20 |
20 |
20 |
25 |
20 |
160 |
0 |
175 |
21 |
20 |
20 |
25 |
20 |
160 |
0 |
175 |
22 |
20 |
20 |
25 |
20 |
160 |
0 |
175 |
23 |
20 |
20 |
25 |
20 |
160 |
0 |
175 |
24 |
20 |
20 |
25 |
25 |
165 |
0 |
180 |
25 |
20 |
20 |
25 |
25 |
165 |
0 |
180 |
26 |
20 |
20 |
25 |
25 |
165 |
0 |
180 |
27 |
20 |
20 |
25 |
25 |
165 |
0 |
180 |
28 |
25 |
25 |
30 |
30 |
170 |
0 |
185 |
Flasks 1 and 2: Acilian
Flasks 3 and 4: inoculum control
Flask 5: reference (procedure control)
Flask 6: abiotic control
Flask 7: toxicity control
Table No.3: Percentage Biodegradation of Test Item, of Sodium Benzoate and of the Toxicity
Control based on ThODNH4
Time days |
FLASK No. |
|||
|
1[%] |
2[%] |
5[%] |
7[%] |
1 |
0 |
0 |
35 |
19 |
2 |
0 |
0 |
50 |
27 |
3 |
0 |
0 |
63 |
34 |
4 |
0 |
0 |
68 |
36 |
5 |
0 |
0 |
71 |
39 |
6 |
0 |
0 |
75 |
40 |
7 |
0 |
0 |
78 |
42 |
8 |
0 |
0 |
79 |
42 |
9 |
0 |
0 |
79 |
|
10 |
0 |
0 |
81 |
42 |
11 |
0 |
0 |
81 |
43 |
12 |
0 |
0 |
84 |
45 |
13 |
0 |
0 |
81 |
45 |
14 |
0 |
0 |
81 |
45 |
15 |
0 |
0 |
81 |
46 |
16 |
0 |
0 |
82 |
47 |
17 |
0 |
0 |
81 |
46 |
18 |
0 |
0 |
81 |
46 |
19 |
0 |
0 |
81 |
48 |
20 |
0 |
0 |
81 |
48 |
21 |
0 |
0 |
81 |
48 |
22 |
0 |
0 |
81 |
48 |
23 |
0 |
0 |
79 |
47 |
24 |
0 |
0 |
82 |
49 |
25 |
0 |
0 |
82 |
49 |
26 |
0 |
0 |
82 |
49 |
27 |
0 |
0 |
82 |
49 |
28 |
0 |
0 |
82 |
49 |
1 ThODNH4 of Acilian: 1.425 mg O2/mg test item
2 ThODNH4 of sodium benzoate: 1.666 mg O2/mg reference item
Table No.4: Percentage Biodegradation of Test Item and of the Toxicity Control based on ThODNO3, and of Sodium Benzoate based on ThODNH4
Time days |
FLASK No. |
|||
|
1[%] |
2[%] |
5[%] |
7[%] |
1 |
0 |
0 |
35 |
17 |
2 |
0 |
0 |
50 |
25 |
3 |
0 |
0 |
63 |
31 |
4 |
0 |
0 |
68 |
33 |
5 |
0 |
0 |
71 |
36 |
6 |
0 |
0 |
75 |
37 |
7 |
0 |
0 |
78 |
39 |
8 |
0 |
0 |
79 |
39 |
9 |
0 |
0 |
79 |
39 |
10 |
0 |
0 |
81 |
40 |
11 |
0 |
0 |
81 |
41 |
12 |
0 |
0 |
84 |
41 |
13 |
0 |
0 |
81 |
41 |
14 |
0 |
0 |
81 |
41 |
15 |
0 |
0 |
81 |
43 |
16 |
0 |
0 |
82 |
44 |
17 |
0 |
0 |
81 |
43 |
18 |
0 |
0 |
81 |
43 |
19 |
0 |
0 |
81 |
44 |
20 |
0 |
0 |
81 |
44 |
21 |
0 |
0 |
81 |
44 |
22 |
0 |
0 |
81 |
44 |
23 |
0 |
0 |
79 |
44 |
24 |
0 |
0 |
82 |
45 |
25 |
0 |
0 |
82 |
45 |
26 |
0 |
0 |
82 |
45 |
27 |
0 |
0 |
82 |
45 |
28 |
0 |
0 |
82 |
45 |
Table No.5: pH values at the End of the Test
Flask No. |
Treatment |
pH-value |
1 |
Anicilian |
7.3 |
2 |
Anicilian |
7.3 |
3 |
Inoculum control |
7.6 |
4 |
Inoculum control |
7.6 |
5 |
Reference item (procedure control) |
7.9 |
Description of key information
Key study: OECD guideline 301F, GLP study. The test substance is considered to be not readily biodegradable under aerobic aqueous conditions.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Key study : The study of the 28 day ready aerobic biodegradability of test item, was carried out according GLP principles and according to OECD 301F/EC C.4 – D manometric respirometry methods, at the temperature 22 °C.The biodegradation was followed by the oxygen uptake of the microorganisms during exposure.The consumption of oxygen was determined by measuring the change of pressure in the flasks.
The evaluation of biodegradation has to be based ThODNH4 and ThODNO3 because test item contains nitrogen. Test item in concentration 102,7 mg/L was inoculated with activated sludge (28.7 mg/L SS) in closed flask at the constant temperature. A reference item was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. A blank test, toxicity test and Abiotic sterile control test were run in parallel. Reference item reach the level of biodegradation within 14 days,the oxygen demand of the blank control was 30 mg O2/L and according the toxicity control the test item can be assumed to be not inhibitory. At the end of the test, at day 28, the degradation of Acilian was 0% (ThODNH4, ThODNO3). Thus the test item cannot be deemed to be readily biodegradable.
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