7-aminonaphthalene-1,3,5-trisulphonic acid

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted following OECD guideline, but report does not mention whether GLP was followed and sufficient data is available for interpretation of results.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

Principles of method if other than guideline:

OECD guideline 209 followed.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

7-aminonaphthalene-1,3,5-trisulphonic acid

EC Number:

248-394-5

EC Name:

7-aminonaphthalene-1,3,5-trisulphonic acid

Cas Number:

27310-25-4

Molecular formula:

C10H9NO9S3

IUPAC Name:

7-aminonaphthalene-1,3,5-trisulfonic acid

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): FAT 93527/A
- Physical state: Powder
- Analytical purity: ca. 50%
- Lot/batch No.: 0031026A0
- Expiration date of the lot/batch: January 10, 2006
- Storage condition of test material: room temperature

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
None

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- Concentrations: 1,10, and 100 mg test item/L
- Sampling method: To obtain the nominal test concentrations of: 1,10, and 100 mg test item/L the following volumes were taken from a stock solution of 0.2g/L: 0.5, 5, and 50 ml_ and filled up to 100 ml_ with tap water after adding of 3.2 mL synthetic sewage feed and 30 ml_ sludge.
- Sample storage conditions before analysis: The test item solutions were prepared close to the test start.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Water
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): To obtain the nominal test concentrations of: 1,10, and 100 mg test item/L the following volumes were taken from a stock solution of 0.2 g/L: 0.5, 5, and 50 ml_ and filled up to 100 ml_ with tap water after
adding of 3.2 mL synthetic sewage feed and 30 ml_ sludge.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Laboratory culture: Not a laboratory culture. -Activated sludge of a communal sewage treatment plant (ARA Therwil) collected on April 09, 2001
- Preparation of inoculum for exposure: The inoculum was prepared 2 days before test begin.
- Pretreatment: The freshly collected activated sludge was washed 3 times with tap water. Subsequently the sludge was aerated. A sample of the activated
sludge was taken to determine the dry weight of the suspended solids.
- Initial biomass concentration: The final concentrations of the inoculum was about 4.0 g/L suspended solids.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

None

Test conditions

Hardness:

No data

Test temperature:

23.2°C

pH:

7.9 - 8

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

nominal test concentrations: 1,10, and 100 mg test item/L

Details on test conditions:

TEST SYSTEM
- Type (delete if not applicable): closed
- Aeration: 0.1 - 0.2 L/min. by Pasteurpipette


TEST MEDIUM / WATER PARAMETERS

Test Medium: A synthetic sewage feed was made by dissolving the following amounts of substances in 1 litre of deionized water:
peptone: 16.0 g
meat extract: 11.0g
urea: 3.0 g
NaCI: 0.7 g
CaCI2. 2H2O: 0.4 g
MgSO4 . 7H2O: 0.2 g
K2HPO4: 2.8 g

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

other: IC20

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed was measured after a contact time of 3 hours. The respiration rate of the same activated sludge in the presence of various concentrations of the test item under otherwise identical conditions was also measured. The inhibitory effect of test item at a particular concentrations was expressed as a percentage of the mean respiration rates of two controls. If possible IC50 and IC20 were calculated from inhibition values at different concentrations.

Results with reference substance (positive control):

None

Reported statistics and error estimates:

No statistics applied

Any other information on results incl. tables

Calculation of the IC50 and IC20 values:

The respiration rate of each test concentration was calculated as a percentage of the mean of the two control respiration rates. Since no 50% and 20% inhibition were observed, the IC50 and IC20 could not be calculated.

Validity of the test:

The test was considered valid because the respiration rates of the two controls were within 15% of each other.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

IC50 > 100 mg/L
IC20 > 100 mg/L

Executive summary:

The objective of this study was the estimation of toxicity of FAT 93527/A to activated sludge containing micro-organisms following OECD Guidelines for the Testing of Chemicals, Number 209. Activated sludge was exposed to different concentrations of the test item. The toxic effect was determined by measuring the respiration rate in relation to controls.

 

Activated sludge of a communal sewage treatment plant (ARA Therwil) collected on April 09, 2001 The inoculum was prepared 2 days before test begin. The freshly collected activated sludge was washed 3 times with tap water. Subsequently the sludge was aerated. A sample of the activated sludge was taken to determine the dry weight of the suspended solids.

 

The final concentrations of the inoculum was about 4.0 g/L suspended solids. To obtain the nominal test concentrations of: 1,10, and 100 mg test item/L the following volumes were taken from a stock solution of 0.2 g/L: 0.5, 5, and 50 ml and filled up to 100 ml with tap water after adding of 3.2 mL synthetic sewage feed and 30 ml sludge.

 

The respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed was measured after a contact time of 3 hours. The respiration rate of the same activated sludge in the presence of various concentrations of the test item under otherwise identical conditions was also measured. The inhibitory effect of test item at a particular concentrations was expressed as a percentage of the mean respiration rates of two controls.

 

The respiration rate of each test concentration was calculated as a percentage of the mean of the two control respiration rates. Since no 50% and 20% inhibition were observed, the IC50and IC20could not be calculated.

Hence it was concluded that:

IC50 (3h) of the test item FAT 93527/A: > 100 mg/L

IC20(3h) of the test item FAT 93527/A: > 100 mg/L