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EC number: 938-925-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From January 4, 2012 to February 2, 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO Standard 10634 "Water Quality - Guidance for the preparation and treatment of poorly water-soluble organic compounds for the subsequent evaluation of their biodegradability in an aqueous medium"
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands.
- Preparation of inoculum for exposure: the sludge was allowed to settle (32 minutes) and the supernatant liquid was used as inoculum.
- Concentration of sludge: 10 mL/L of mineral medium.
- Initial cell/biomass concentration: 3.4 g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 14.6 mg/L
- Based on:
- other: TOC
- Initial conc.:
- 14.65 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
1 litre mineral medium contains: 10 mL of solution (A), 1 mL of solutions (B) to (D) and Milli-RO water.
A) 8.50 g KH2PO4
21.75 g K2HPO4
67.20 g Na2HPO4.12H2O
0.50 g NH4Cl
dissolved in Milli-Q water and made up to 1 litre, pH 7.4 ± 0.2
B) 22.50 g MgSO4.7H2O dissolved in Milli-Q water and made up to 1 litre.
D) 0.25 g FeCl3.6H2O dissolved in Milli-Q water and made up to 1 litre.
- Additional substrate: 0.0125 M Ba(OH)2 (Boom, The Netherlands), stored in a sealed vessel to prevent absorption of CO2 from the air.
- Test temperature: 21.9 - 22.9ºC
- pH: 7.5 - 7.8
- pH adjusted: no
- Aeration of dilution water: The pre-incubation medium was aerated with synthetic air overnight.
- Suspended solids concentration: 3.4 g/L
- Continuous darkness: no
TEST SYSTEM
- Culturing apparatus: 2 litre all-glass brown coloured bottles.
- Number of culture flasks/concentration: 1
- Measuring equipment: CO2-absorber.
- Details of trap for CO2 and volatile organics if used: Each time the CO2-absorber nearest to the test bottle was removed for titration; each of the remaining two absorbers was moved one position in the direction of the test bottle. A new CO2-absorber was placed at the far end of the series. Phenolphthalein (1% solution in ethanol, Merck) was used as pH-indicator.
SAMPLING
- Sampling frequency: Titrations were made every second or third day during the first 10 days, and thereafter at least every fifth day until the 28th day, for the inoculum blank and test suspension. Titrations for the positive and toxicity control were made at least 14 days.
- Sampling method: The CO2 produced in each test bottle reacted with the barium hydroxide in the gas scrubbing bottle and produced was determined by titrating the remaining Ba(OH)2 with 0.05 M standardized HCl.
CONTROL AND BLANK SYSTEM
- Inoculum blank: only inoculum.
- Toxicity control: test substance, reference substance and inoculum.
- Other :Positive control: reference substance and inoculum. - Reference substance:
- other: Sodium acetate
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 64
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 62
- Sampling time:
- 28 d
- Details on results:
- The final degradation value after the test period of 28 days was 63% (mean value of two parallel test solution). The toxicity control was degraded 44% within 14 days and showed that no toxicity of the test subtance has reduced the activity of the inoculum. The CO2 evolution measured in the blank was in the required range.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Reaction products of n-octanol and acrylic acid, first distillation pitch was readily biodegradability (62-64%).
- Executive summary:
The test substance was tested for ready biodegradability according to OECD 301B Guideline, CO2 evolution test (with GLP). Since the organic carbon content could not be calculated, a sample of the pure test substance was taken for determination of the Total Organic Carbon (TOC) content (to be 81%). Based on the TOC content the ThCO2 was calculated to be 2.99 mg CO2/mg.
The relative biodegradation values calculated from the measurements performed during the test period revealed 64 and 62% biodegradation of test substance, for A and B, respectively. Furthermore, the batch of Reaction products of n-octanol and acrylic acid, first distillation pitch is an UVCB and therefore the 10-day window was not applied.
In the toxicity control, test substance was found not to inhibit microbial activity. Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, the test substance was designated as readily biodegradable.
Reference
Table 1. CO2production and percentage biodegradation of the test substance (bottle A).
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2(mg) |
Cumulative CO2(mg) |
Biodegradation1 (%) |
|
Blank (mean) |
bottle A |
|||||
2 |
48.03 |
47.83 |
0.20 |
0.2 |
0.2 |
0 |
5 |
47.41 |
43.45 |
3.96 |
4.4 |
4.6 |
5 |
7 |
47.57 |
43.13 |
4.44 |
4.9 |
9.4 |
11 |
9 |
47.26 |
41.71 |
5.55 |
6.1 |
15.6 |
18 |
14 |
45.79 |
38.18 |
7.61 |
8.4 |
23.9 |
27 |
19 |
44.09 |
36.84 |
7.25 |
8.0 |
31.9 |
37 |
23 |
44.17 |
36.68 |
7.49 |
8.2 |
40.1 |
46 |
27 |
45.07 |
38.64 |
6.43 |
7.1 |
47.2 |
54 |
29 |
46.25 |
41.63 |
4.62 |
5.1 |
52.3 |
60 |
29 |
48.67 |
45.98 |
2.69 |
3.0 |
55.2 |
63 |
29 |
49.96 |
49.58 |
0.38 |
0.4 |
55.7 |
64 |
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test substance: 87.3 mg CO2/2L
Table 2. CO2production and percentage biodegradation of the test substance (bottle B).
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2(mg) |
Cumulative CO2(mg) |
Biodegradation1 (%) |
|
Blank (mean) |
bottle B |
|||||
2 |
48.03 |
47.53 |
0.49 |
0.5 |
0.5 |
1 |
5 |
47.41 |
43.33 |
4.08 |
4.5 |
5.0 |
6 |
7 |
47.57 |
43.30 |
4.27 |
4.7 |
9.7 |
11 |
9 |
47.26 |
42.60 |
4.66 |
5.1 |
14.9 |
17 |
14 |
45.79 |
38.10 |
7.69 |
8.5 |
23.3 |
27 |
19 |
44.09 |
37.19 |
6.90 |
7.6 |
30.9 |
35 |
23 |
44.17 |
36.82 |
7.35 |
8.1 |
39.0 |
44 |
27 |
45.07 |
38.40 |
6.67 |
7.3 |
46.3 |
53 |
29 |
46.25 |
41.47 |
4.78 |
5.3 |
51.6 |
59 |
29 |
48.67 |
46.30 |
2.37 |
2.6 |
54.2 |
62 |
29 |
49.96 |
50.00 |
0.00 |
0.0 |
54.2 |
62 |
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test substance: 87.6 mg CO2/2L
Description of key information
Key study: OECD Guideline 301 B. GLP study. The aerobic biodegradation of test substance after 28 days of incubation attained 63% (mean) and was determined to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Key study: Experimental result on test item Reaction products of n-octanol and acrylic acid, first distillation pitch:
The readily biodegradability in an aerobic aqueous medium with Carbon Dioxide (CO2) evolution test (modified Sturm Test) was carried out according to the OECD Guideline 301B (GLP study). The aerobic biodegradation of test substance (14.6 mg/L) after 28 days of incubation with activated sludge (3.4 g/L SS) attained 63% (mean) based on CO2 evolution. Therefore, the test substance was determined to be readily biodegradable in aerobic aqueous conditions.
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