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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 23-25, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- solid: granular
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Mitsubishi-Chemical Foods Corporation; 6912911B
- Expiration date of the lot/batch: September 11, 2018
- Purity test date: June 27, 2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: below 15 degrees C in a cool, dark, well-ventilated space with container tightly closed
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 200 mg of test substance was added to 2 L of dilution water. This was stirred for 2 hrs at room temperature. Another stock solution was prepared for renewal at 24 hrs in a similar manner. The stock solution served as the highest concentration. Additional test concentration were prepared by successive dilutions of the stock solution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: German Federal Environmental Agency, Institut fur Wasser-, Boden- und Lufthygiene
- Age of parental stock (mean and range, SD): 3 weeks
- Feeding during test: No
ACCLIMATION
- Acclimation period: 4-24 hrs
- Acclimation conditions (same as test or not): yes
- Type and amount of food: algal suspension and ArtemioFluid
- Feeding frequency: daily
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: newborns were seperated by sieving and transferred with a bore Pasteur pipette
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 140-250 mg/L CaCO3
- Test temperature:
- 20.8 degrees C
- pH:
- 7.62-8.34
- Dissolved oxygen:
- 3-9 mg/L
- Nominal and measured concentrations:
- Nominal: 0, 6.25, 12.5, 25.0, 50.0, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Type (delete if not applicable): open - covered with glass panes to prevent evaporation but allow gas exchange
- Material, size, headspace, fill volume: glass, 50 mL fill volume
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): 24 hrs
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified drinking water, Cu-reduced
OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs dark
- Light intensity: 814-823 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilization, 0, 24, and 48 hrs
RANGE-FINDING STUDY
- Test concentrations: 1.0, 10.0, 100 mg/L
- Results used to determine the conditions for the definitive study: In both the 1.0 and 10.0 mg/L concentrations, one daphnid died. At the 100 mg/L concentration, two daphnids died. As the observed effects were not statistically significant, a test range of 0-100 mg/L was considered appropriate. - Reference substance (positive control):
- yes
- Remarks:
- Test sensitivity of the test organism is regularly tested using K2Cr2O7.
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: one daphnid in the 100 mg/L concentration was immobilized
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 5% immobilization at 0.90 mg/L, 50% immobilization at 1.35 mg/L, 75% immobilization at 2.00 mg/L
- Limit test: no
- Dose-response test: yes
- ECx: EC50 = 1.435 mg/L - Reported statistics and error estimates:
- No statistics were calculated as the LC50 could not be calculated.
Any other information on results incl. tables
Daphnid Immobilization
Concentration (mg/L) |
Total Daphnids |
Mobile |
Immobile |
% Immobility |
0 |
20 |
20 |
0 |
0.0 |
6.25 |
20 |
20 |
0 |
0.0 |
12.5 |
20 |
20 |
0 |
0.0 |
25.0 |
20 |
20 |
0 |
0.0 |
50.0 |
20 |
20 |
0 |
0.0 |
100 |
20 |
19 |
1 |
5.0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hr LC50 > 100 mg/L.
- Executive summary:
The toxicity of the test substance to aquatic invertebrates was determined in an OECD Guideline 202 test. Daphnids were exposed to test concentrations of 0, 6.25, 12.5, 25.0, 50.0, and 100 mg/L, with renewal at 24 hrs. Due to difficulties in analyzing the test substance, analytical concentrations were not determined. Rather, nominal concentrations were reported. Only one daphnid was immobilized at the 100 mg/L concentration. At all other concentrations, no daphnids showed immobility. The LC50 > 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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