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EC number: 947-862-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Dec - 20 Dec 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fatty acids C8-18 (even numbered), mono- and diesters with sucrose
- EC Number:
- 947-862-2
- Molecular formula:
- not applicable, substance is UVCB
- IUPAC Name:
- Fatty acids C8-18 (even numbered), mono- and diesters with sucrose
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All test concentrations incl. control
- Sampling method: Samples were taken at the beginning and end of exposure period.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test sample (0.150 g) was dissolved in dilution water (1500 mL) to prepare the stock solution of 100 mg/L. Required volumes of the stock solution and dilution water were mixed and stirred in a preparation container to prepare the test solution which was divided into each test vessel. The 100 mg/L test solution was prepared by direct addition.
- Eluate: no
- Differential loading: no (except for the highest test concentration)
- Controls: yes, test medium control
- Evidence of undissolved material: no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: The University of Sheffield, UK (obtained 9 July 1990)
- Age of daphnids: < 24 h
- Feeding during test: none
ACCLIMATION
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Chlorella vulgaris of 0.1-0.2 mg C (Organic carbon content)/day.
- Feeding frequency: daily
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 32 mg/L
- Test temperature:
- 19.9 - 20.2 °C
- pH:
- 7.8 - 8.0
- Dissolved oxygen:
- 8.7 - 9.0 mg O2/L
- Conductivity:
- 13 mS/m
- Nominal and measured concentrations:
- nominal: control, 9.53, 17.1, 30.9, 55.6, 100 mg/L
Measured (geometric mean): < LOD, 8.65, 16.1, 29.6, 52.1, 97.0 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beaker covered with a transparent plastic lid (incubated in a water bath with automatic warming/cooling unit)
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Culture medium different from test medium: no
- Intervals of water quality measurement: Water quality was measured in regular intervals (temperature, O2, pH).
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h darkness
- Light intensity: room light
EFFECT PARAMETERS MEASURED
- Immobilisation was measured after 24 and 48 h.
RANGE-FINDING STUDY
- Test concentrations: 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: 100% immobilisation was recorded at 100 mg/L whereas no immobilisation was observed at 1 and 10 mg/L. The measured test concentrations during range finder were in the range of 93.2 - 105% of nominal. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate was periodically tested in a 48 hour test design.
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 74.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test solutions were clear and colorless. - Results with reference substance (positive control):
- - Results with reference substance valid?
yes
- Relevant effect levels: EC50 (48 h) 0.12 mg/L
- Other: Test was conducted from 18 - 20 Oct 2017. - Reported statistics and error estimates:
- The EC50 value was calculated using Excel by binominal analysis.
Any other information on results incl. tables
The measured test concentrations were in the range of 80 - 120% of nominal (81.5 - 101%; see Table 2). Thus, it is justified that the effect concentrations were based on the nominal concentrations.
Table 1: Immobilisation of Daphnia magna after 24 and 48 h of exposure.
Nominal concentration [mg/L] |
Vessel |
24 h |
48 h |
||
Number of immobilized Daphnids/total Daphnids |
% |
Number of immobilized Daphnids/total Daphnids |
% |
||
Control |
A |
0 / 5 |
0 |
0 / 5 |
0 |
B |
0 / 5 |
0 / 5 |
|||
C |
0 / 5 |
0 / 5 |
|||
D |
0 / 5 |
0 / 5 |
|||
9.53 |
A |
0 / 5 |
0 |
0 / 5 |
0 |
B |
0 / 5 |
0 / 5 |
|||
C |
0 / 5 |
0 / 5 |
|||
D |
0 / 5 |
0 / 5 |
|||
17.1 |
A |
0 / 5 |
0 |
0 / 5 |
0 |
B |
0 / 5 |
0 / 5 |
|||
C |
0 / 5 |
0 / 5 |
|||
D |
0 / 5 |
0 / 5 |
|||
30.9 |
A |
0 / 5 |
0 |
0 / 5 |
0 |
B |
0 / 5 |
0 / 5 |
|||
C |
0 / 5 |
0 / 5 |
|||
D |
0 / 5 |
0 / 5 |
|||
55.6 |
A |
0 / 5 |
0 |
0 / 5 |
0 |
B |
0 / 5 |
0 / 5 |
|||
C |
0 / 5 |
0 / 5 |
|||
D |
0 / 5 |
0 / 5 |
|||
100 |
A |
3 / 5 |
80 |
5 / 5 |
100 |
B |
5 / 5 |
5 / 5 |
|||
C |
4 / 5 |
5 / 5 |
|||
D |
4 / 5 |
5 / 5 |
Table 2: Measured concentrations (mg/L + % of nominal) after 0 and 48 h of exposure)
Nominal concentration [mg/] |
Measured concentration [mg/L] (%) |
||
0 h |
48 h |
Geometric mean |
|
Control |
Not detected |
Not detected |
- |
9.53 |
9.63 (101) |
7.77 (81.5) |
8.65 (90.7) |
17.1 |
17.1 (100) |
15.1 (88.3) |
16.1 (94.1) |
30.9 |
30.8 (99.8) |
28.4 (91.9) |
29.6 (95.8) |
55.6 |
56.3 (101) |
48.3 (86.9) |
52.1 (93.8) |
100 |
97.9 (97.9) |
96.1 (96.1) |
97.0 (97.0) |
Table 3: Validity criteria for OECD 202.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
8.7 - 9.0 mg/L |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
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