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EC number: 640-454-2 | CAS number: 17318-08-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was directly pipetted into each test flask. The weight was recorded and filled up with pure water.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Bensheim, Germany
- Method of cultivation: the activated sludge was used as collected, but coarse particles were removed by settling for a short period (15 minutes) and then the upper layer decanted. During holding prior to use for one day (dose-response study) or two days (pre-test), the sludge was fed daily with 50 mL synthetic sewage feed per litre and kept aerated at room temperature until use - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 3 °C
- pH:
- Control: 6.9 to 7.1 at start and 7.5 to 7.9 at end
Positive control: 6.9 at start and 7.7 to 7.8 at end
Test solutions: 6.8 to 7.0 at start and 7.2 to 7.4 at end - Dissolved oxygen:
- Control: 6.1 to 6.8 mg O2/L at start and 5.8 to 7.7 mg O2/L at end
Positive control: 6.3 to 6.5 O2/L at start and 7.7 to 8.3 mg O2/L at end
Test solutions: 6.4 to 7.0 mg O2/L at start and 6.6 to 7.2 mg O2/L at end - Nominal and measured concentrations:
- Nominal: 10, 32, 100, 320 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: all
- Type: open
- Material, size, headspace, fill volume: Glass flasks of approximately 1 L volume, final test volume of 500 mL per flask
- Aeration: during the holding period and during the 3 hours of the respiration inhibition test, the sludge was aerated with compressed air (1.017 L/minute)
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 6
- No. of vessels per positive control (replicates): 5
- No. of vessels per abiotic control (replicates): not applicable
- Sludge concentration (weight of dry solids per volume): 3 g/L
- Nutrients provided for bacteria: synthetic sewage was prepared by mixing 80 g peptone, 55 g meat extract, 15 g urea, 3.5 g NaCl, 2.0 g CaCl2 x 2 H2O, 1.0 MgSO4 x 7 H2O, 14 g K2HPO4 filled up with 5 L of deionised water
- Nitrification inhibitor used: N-allylthiourea
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water
- Particulate matter: not reported
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Details on termination of incubation: For the measurement of the respiration rate a well-mixed sample of test medium from each flask was poured into a Karlsruher flask after exactly 3 hours incubation time and was not further aerated during measurement. The oxygen concentration was then measured with an oxygen electrode and recorded for about ten minutes. During measurement, the samples were continuously stirred on a magnetic stirrer.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3
- Range finding study
- Test concentrations: 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 85.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 909.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of respiration due to nitrification
- Results with reference substance (positive control):
- Total respiration EC50 = 2.5 mg/L
Heterotrophic respiration EC50 = 14.8 mg/L
Nitrification respiration EC50 = 0.9 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on total respiration, the test substance had no effects on activated sludge micro-organisms up to a concentration of 100 mg/L and significant dose-response related effects on total respiration at concentrations of 320 and 1000 mg/L. Therefore, the NOEC for respiration inhibition was 100 mg/L, and the EC50 was >1000 mg/L.
- Executive summary:
A respiration inhibition study with activated sludge from a sewage treatment plant receiving domestic waste water was conducted in accordance with OECD TG 209 under GLP. The 3-hour toxicity test had the purpose to evaluate the influence of the test substance on the activity of the activated sludge by measuring the respiratin rate under defined conditions. The effects on the activated sludge were studied in a pre-test and a main test. For each replicate, a test solution with a final volume of 500 mL was tested in a 1 L glass flask: 16 mL of synthetic sewage feed and an adequate volume of the test substance or of the stock solution of the reference substance was directly pipetted into each test flask, which was then filled up with pure water to 250 mL. At the start of the test, 250 mL of activated sludge inoculum with a sludge concentration of 3.0 g/L suspended solids was added. The
Reference
Description of key information
Respiration inhibition: 3-hour NOEC = 100 mg/L, 3-hour EC50 >1000 mg/L in activated sludge (GLP, OECD TG 209)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
The result of the key study was confirmed in a new study conducted to meet the data requirements for registration of the substance in a non-EEA country, which gave an EC50 value for the inhibition of total respiration of >1000 mg/L.
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