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Diss Factsheets
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EC number: 911-418-6 | CAS number: 55965-84-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- Version / remarks:
- Part B
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Test material form:
- other: Amber liquid
- Details on test material:
- - Name of test material (as cited in study report):Kathon(TM) 886F biocide
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Rohm and Haas, Batch No. 6111L019
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: Stable at room temperature
- Solubility and stability of the test substance in the solvent/vehicle: Soluble and stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: No
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dilution in water
- Final dilution of a dissolved solid, stock liquid or gel: 62.5, 125, 250, 500 and 1000 mg a.i./L
OTHER SPECIFICS: Purity of test material was 14.2%, of which 10.8% CMIT and 3.4% MIT
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 62.5, 125, 250, 500 and 1000 mg ai/L
- Sampling method: Dry weights were determined from plant shoots collected on day 21
- Sample storage conditions before analysis: Not described
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of application to soil surface (if used): Foliar application
- Controls: negative control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Water
- Concentration of vehicle in test medium (stock solution and final test solution): Not applicable
- Evaporation of vehicle before use: Not applicable
Test organisms
open allclose all
- Species:
- Trifolium pratense
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Red clover
- Species:
- Oryza sativa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Rice
- Species:
- Brassica napus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Oilseed rape
Study design
- Test type:
- vegetative vigour test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- Mean: 22.98 C
- pH:
- 7.5
- Moisture:
- Mean: 74.76 %
- Details on test conditions:
- TEST SYSTEM
- Testing facility: Greenhouse
- Test container (type, material, size): Plastic pots (10 cm x 11 cm)
- Amount of soil: Not described
- Method of seeding: Not described
- No. of seeds per container: Not described
- No. of plants (retained after thinning): 5
- No. of replicates per treatment group: 6
- No. of replicates per control: 6
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Not described
- Pesticide use history at the collection site: Not described
- Collection procedures: Not described
- Sampling depth (cm): Not described
- Soil texture (if natural soil)
- % sand: 53%
- % silt: 30%
- % clay: 17%
- Soil taxonomic classification: Sandy loam
- Soil classification system: Not described
- Organic carbon (%): 1.1 %
- Maximum water holding capacity (in % dry weigth): Not described
- CEC: Not described
- Pretreatment of soil: Not described
- Storage (condition, duration): Not described
GROWTH CONDITIONS
- Photoperiod: 14 hour photoperiod
- Light source: Not described
- Light intensity and quality: Mean moles photosynthetically active radiation: 13.2
- Day/night temperatures: Mean: 22.98 °C
- Relative humidity (%): Mean: 74.76 %
- Wind velocity: Not described
- Air exchange rate: Not described
- Watering regime and schedules: By sub-irrigation once or twice weekly
- Water source/type: Well water
- Volume applied: ca. 200 L/ha
- Interval of applications: Single application
- Method of application: Spray
- Any pest control method/fertilization (if used): Seeds were not pretreated with insecticides or fungicides.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observation periods: 0, 7, 14 and 21 days, seedling condition. Dry weights were determined from plant shoots collected on day 21.
VEHICLE CONTROL PERFORMED: yes - Nominal and measured concentrations:
- Nominal: 0, 62.5, 125, 250, 500 and 1000 mg a.i./L
Mean measured:- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: highest concentration tested
- Key result
- Species:
- Oryza sativa
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: highest concentration tested
- Key result
- Species:
- Trifolium pratense
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: highest concentration tested
- Details on results:
- VEGETATIVE VIGOUR
- Percent seed germination: Not examined
- Plant height: No effects
- Dry weight: No effects
- Abnormal seed development or appearance: Not described
- Lesions: Not described
- Swelling: Not described
- Loss of turgour: Not described
- Discoloration: Not described
- Unusual leaf/plant shape or size: Not described
- Dead plants: No effects
INJURY RATING SYSTEM: No apparent signs of treatment-related phytotoxicity to any of the three species - Reported statistics and error estimates:
- Statistical analyses were used to evaluate effects of TS application on plant biomass and survival. Analyses were performed on data from the test using the Dunnett option of the GLM (general linear model) procedure of SAS version 8 (SAS Institute, Inc. 1999. SAS Proprietary Software Version 8, Cary, North Carolina, USA). Significance was determined at the level of 0.05 and 0.01 (Dunnett’s test, p < 0.05, p < 0.01). Test data were evaluated to determine the LOEC and NOEC for plant biomass. Dunnett’s test was used to establish the LOEC and NOEC by determining which treatment groups differed significantly (p < 0.05, p < 0.01) from the control group.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 21d NOEC = 1000 mg a.i./L, the highest concentration tested
- Executive summary:
OECD Draft Guideline 208, Part B, terrestrial non-target plant test: vegetative vigour test with analytical confirmation of dosing solutions. No apparent signs of treatment-related phytotoxicity to any of the three species. 21d NOEC = 1000 mg a.i./L, the highest concentration tested.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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