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EC number: 283-393-3 | CAS number: 84608-82-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 Nov - 11 Dec 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- Version 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- Version 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Energie, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany
Test material
- Reference substance name:
- 2-hydroxy-3-(oleoyloxy)propyl 5-oxo-L-prolinate
- EC Number:
- 283-393-3
- EC Name:
- 2-hydroxy-3-(oleoyloxy)propyl 5-oxo-L-prolinate
- Cas Number:
- 84608-82-2
- Molecular formula:
- C26H45NO6
- IUPAC Name:
- 2-hydroxy-3-(oleoyloxy)propyl 5-oxo-L-prolinate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Aerobic activated sludge (microorganisms from a domestic wastewater treatment plant) was supplied by the sewage works of Rossdorf, Germany.
- Preparation of inoculum for exposure: The aerobic activated sludge was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and centrifuged again. This procedure was done three times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material
per litre were mixed with test water. This suspension was used for the experiment.
- Concentration of sludge: 28.7 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 104.1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 238.6 mg/L
- Based on:
- other:
- Remarks:
- ThOD NH4
- Initial conc.:
- 252.9 mg/L
- Based on:
- other:
- Remarks:
- ThOD NO3
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Solubilising agent: For a better distribution of the test item, silicone oil AR20 with a concentration of about 1% was added to the test flasks.
- Test temperature: 22 ± 1°C
- pH: 7.6 (measured at test start); 7.1-7.5 (measured at test end)
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 2 flasks with test item (25.6 and 25.2 mg per flask with 244 mL final volume each)
- Measuring equipment: BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany. The amount of O2 consumed by the activated sludge was calculated from the decrease of pressure in the reaction vessel. The change of pressure in the test flasks was measured by means of a manometric method (BSB/BOD-Sensor-System) each day.
- Test performed in closed vessels: The test flasks were closed gas-tight by a measuring head.
- Details of trap for CO2 and volatile organics: Potassium hydroxide solution (45%) was used for trapping the produced carbon dioxide.
SAMPLING
- Sampling frequency and method: Samples were taken every day from both test flasks, the toxicity and the reference control.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 flasks
- Abiotic control: yes, 1 flask (25.0 mg test item per flask with 246.4 mL final volume)
- Toxicity control: yes, 1 flask (25.5 mg test item and 25.3 mg reference item per flask with 244 mL final volume)
- Reference control: yes, 1 flask (25.2 mg reference item per flask with 244 mL final volume)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 80
- Sampling time:
- 28 d
- Remarks on result:
- other: based on ThOD NH4
- Parameter:
- % degradation (O2 consumption)
- Value:
- 76
- Sampling time:
- 28 d
- Remarks on result:
- other: based on ThOD NO3
- Details on results:
- The mean biodegradation percentage based on ThOD NO3 at the end of the 10-day window was 70%; the criterion of the 10 day window was passed. Nitrogen is part of the molecular structure of the test item; therefore nitrification (ThOD NO3) was considered for the evaluation of results.
The toxicity control reached 58% degradation after 14 days. The substance is therefore not considered inhibitory to microorganisms.
BOD5 / COD results
- Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 77% after 14 days and to 80% after 28 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
Any other information on results incl. tables
Table 1:Percentage Biodegradation of Test Item, of Sodium Benzoate and of the Toxicity Control based on ThOD NH4
Time (days) |
Percentage Biodegradation |
|||
Test item |
Reference substance |
Toxicity control |
||
Flask 1 [%] |
Flask 2 [%] |
Flask 5 [%] |
Flask 7 [%] |
|
1 |
12 |
13 |
32 |
17 |
2 |
19 |
21 |
41 |
25 |
3 |
29 |
32 |
64 |
40 |
4 |
40 |
42 |
67 |
45 |
5 |
48 |
53 |
70 |
50 |
6 |
56 |
61 |
73 |
53 |
7 |
60 |
65 |
73 |
55 |
8 |
67 |
70 |
73 |
56 |
9 |
69 |
72 |
73 |
57 |
10 |
73 |
74 |
76 |
58 |
11 |
72 |
75 |
74 |
59 |
12 |
73 |
76 |
76 |
58 |
13 |
75 |
76 |
76 |
58 |
14 |
74 |
77 |
77 |
58 |
15 |
75 |
78 |
76 |
57 |
16 |
75 |
80 |
76 |
57 |
17 |
77 |
80 |
76 |
57 |
18 |
77 |
80 |
78 |
57 |
19 |
76 |
81 |
77 |
56 |
20 |
78 |
81 |
77 |
56 |
21 |
78 |
83 |
77 |
56 |
22 |
78 |
83 |
77 |
56 |
23 |
78 |
83 |
77 |
56 |
24 |
78 |
83 |
80 |
56 |
25 |
78 |
83 |
80 |
56 |
26 |
80 |
83 |
80 |
56 |
27 |
78 |
81 |
80 |
56 |
28 |
78 |
81 |
80 |
56 |
Table 2: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
1.9% |
yes |
Percentage degradation of the reference compound has reached the pass levels by day 14. |
60% reached after 3 days |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
58% |
yes |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg/L in 28 days. |
27.5 mg/L |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Interpretation of results:
- readily biodegradable
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