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EC number: 269-923-6 | CAS number: 68391-04-8 This substance is identified by SDA Substance Name: C12-C18 alkyl dimethyl amine and SDA Reporting Number: 16-040-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: short-term (with study design considered suitable for long-term assessment)
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Justification for type of information:
- For details on endpoint-specific justification, please see read-across justification document (category approach) in the linked category of dimethylalkylamines.
- Analytical monitoring:
- no
- Vehicle:
- yes
- Species:
- other: Gramineae
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- Avena sativa (oat)2, Flämingstern
- Species:
- other: Brassicaceae
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Brassica napus (rape)2, Petronova
- Species:
- other: Leguminosae
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Trifolium pratense (red clover)1, Viola
- Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- other: Natural soil: Certified LUFA soil No. 2.3 (batch-no. Sp2.35000)
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- No
- Test temperature:
- 20±10
- pH:
- 6.5±0.2
- Moisture:
- See "Any other information on materials and methods inc. tables"!
- Nominal and measured concentrations:
- Nominal concentrations:
Conc. (mg/kg dry weight soil) : 10, 32, 100, 320, 1000
No analytical determination preformed! - Reference substance (positive control):
- yes
- Remarks:
- 8 replicates applied with 28.6 g lyophilised sewage sludge and 160 g quarts sand
- Key result
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 11.1 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: RA from C12-14-DMA; value converted based on MW
- Basis for effect:
- other: Shoot height
- Remarks on result:
- other: Orig. value source: 10 mg/kg dw
- Details on results:
- See tables below!
- Results with reference substance (positive control):
- No reference substance
- Reported statistics and error estimates:
- NOEC of biomass/ emerged seedlings:
The NOEC was determined for inhibition of shoot height, inhibition of fresh weight and the rate of emergence. Dead plants were measured, weighed and observed and included in the statistical analysis if possible.
One way analysis of variance (ANOVA) and DUNNETT'S test was carried out for the determination of statistically significant differences compared to control replicates. When running a one way analysis of variance a normality test and an equal variance test were done first. P-values for both normality and equal variance test are 0.05. The a-value for ANOVA (acceptable probability of incorrectly concluding that there is a difference) is a = 0.05.
EC-values and confidence ranges of growth:
Calculation was carried out via probit analysis. Probit values according to WEBER (1986). Calculation of the confidence intervals for EC 50 were carried out using standard procedures according to BREITIG & TÜMPLING (1982).
BREITIG & TÜMPLING (1982): Methoden der Wasseruntersuchungen Bd.11., VEB GUSTAV FISCHER VERLAG, Jena.
WEBER, E., Grundrifl der biologischen Statistik. 9. Auflage (1986). - Validity criteria fulfilled:
- no
- Remarks:
- For red clover less than 70% seedling ermgence for control (55%). This criterion was not yet established in the draft guideline version.
- Executive summary:
The study used as source investigated the effects of C12-14-DMA towards terr. plants in a Seedling Emergence and Seedling Growth test (OECD 208). The study results of the source substance were considered applicable to the target substance. Justification and applicability of the read-across approach (category approach) is outlined in the read-across report in the linked category of dimethylalkylamines.
Reference
shoot height
Species | NOEC* (mg/kg DW) | EC25 (mg/kg DW) | p= 95% (mg/kg DW) | EC50(mg/kg DW) | p=95% (mg/kg DW) |
oat | 320 | 526 | 360-769 | >1000 | 823- >1000 |
rape | 10 | 52 | 38-71 | 120 | 88-165 |
red clover | 32 | 114 | 101-130 | 159 | 141-180 |
fresh weight
Species | NOEC* (mg/kg DW) | EC25 (mg/kg DW) | p= 95% (mg/kg DW) | EC50(mg/kg DW) | p=95% (mg/kg DW) |
oat | 100 | 473 | 408-548 | 700 | 604-811 |
rape | 100 | 53 | 40-70 | 127 | 96-169 |
red clover | 32 | 129 | 112-149 | 188 | 164-217 |
seed emergence
Species | NOEC* (mg/kg DW) | EC25 (mg/kg DW) | p= 95% (mg/kg DW) | EC50(mg/kg DW) | p=95% (mg/kg DW) |
oat | 320 | 671 | 555-812 | >1000 | - |
rape | 320 | 402 | 338-478 | 586 | 493-697 |
red clover | 320 | 101 | 77-133 | 237 | 180-313 |
- not determinable
* the highest tested concentration of the test item at which no statiscally significant effect is observed
Phytotoxic effect: decrease in lenght | |||||
Species | NOEC* (mg/kg DW) | EC25 (mg/kg DW) | p= 95% (mg/kg DW) | EC50(mg/kg DW) | p=95% (mg/kg DW) |
oat | 320 | >1000 | - | >1000 | - |
rape | 100 | 233 | 214-254 | 281 | 258-306 |
red clover | n.o | n.o | - | n.o | - |
Phytotoxic effect: deformation | |||||
Species | NOEC* (mg/kg DW) | EC25 (mg/kg DW) | p= 95% (mg/kg DW) | EC50(mg/kg DW) | p=95% (mg/kg DW) |
oat | 320 | >1000 | - | >1000 | - |
rape | n.o | n.o | - | n.o | - |
red clover | n.o | n.o | - | n.o | - |
Phytotoxic effect: chlorosis | |||||
Species | NOEC* (mg/kg DW) | EC25 (mg/kg DW) | p= 95% (mg/kg DW) | EC50(mg/kg DW) | p=95% (mg/kg DW) |
oat | n.o | n.o | - | n.o | - |
rape | 100 | 275 | 164-460 | 844 | 504- >1000 |
red clover | n.o | n.o | - | n.o | - |
n.o: not observed (effects < 10% were not considered)
- not determinable
* The higutest tested dose of the test item at which no statistically significant effect is observed
Description of key information
In a reliable study (reliability category 2) on C12-14 DMA assessing toxicity in regard to seedling emergence, phytotoxic effects, shoot height and shoot fresh weight for three plant species, the most sensitive endpoint was shoot height for rape: NOEC (21 d) = 0.045 mmol/kg soil dry weight.
Key value for chemical safety assessment
- Long-term EC10, LC10 or NOEC for terrestrial plants:
- 8.41 mg/kg soil dw
Additional information
Toxicity of C12-14 DMA in regard to seedling emergence, phytotoxic effects, shoot height and shoot fresh weight of terrestrial plants was assessed in a reliable (category 2) seedling emergence test over a period of 21 days (OECD 208, compliant to GLP) at nominal concentrations of 10 -32 -100 -320 -1000 mg/kg soil dry weight (DW). Tested plant species were the monocotyledon oats (Gramineae) and the two dicotyledons rape (Brassicaceae) and red clover (Leguminosae). Validity criteria of the OECD-guideline were met, except for control seedling emergence of red clover (<70%; reason for RL 2 – no other restrictions regarding reliability). The following NOECs (21 d) were determined:
Shoot height: oat 320 mg/kg DW; rape 10 mg/kg DW; red clover 32 mg/kg DW
Fresh weight: oat 100 mg/kg DW; rape 100 mg/kg DW; red clover 32 mg/kg DW.
Phytotoxic effects: oat (decrease in length) 320 mg/kg DW; rape (decrease in length) 100 mg/kg DW; rape (chlorosis) 100 mg/kg DW
Thus, the lowest NOEC (21 d) of 0.045 mmol/kg soil dry weight (10 mg C12-14-DMA)/kg dry weight was determined for shoot height of rape, and this NOEC will be the decisive value for PNECsoil derivation for DMA category members due to plants being more sensitive to DMA than soil microorganisms or terrestrial macroorganisms.
However, molar concentrations cannot be used for environmental hazard and risk assessment. Therefore, in a conservative way the molecular weight of C10-DMA is used to transform the molar into mass-based concentration:
Key value for terrestrial plant toxicity of DMA category members: NOEC (21 d; Brassica napus; shoot height) = 8.41 mg/kg soil dry weight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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