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Diss Factsheets
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EC number: 227-105-6 | CAS number: 5657-17-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Peer-reviewed data from EU Risk Assessment Report
- Justification for type of information:
- Study is read across and slightly updated and presented under "type of information" as experimental study until the lead company has updated the Source RSS
Read across justification is presented in IUCLID chapter 13 - Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Equimolar amounts of CaCl2 were added, thus CaEDTA was formed in the stock solutions.
- Test organisms (species):
- activated sludge, domestic
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Nominal and measured concentrations:
- Na2H2EDTA was used in concentrations of 125, 250, and 500 mg/L (referred as H4EDTA).
- Details on test conditions:
- Type: aquatic
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- At the highest test concentration (500 mg/L) a significant inhibition of the respiration rate could not be detected after 30 minutes. It can be concluded that both the EC10 and NOEC values are above 500 mg/L.
Reference
Description of key information
The inhibition of the degradation activity of activated sludge is not anticipated when the test substance introduced in appropriate low concentrations
Key value for chemical safety assessment
- EC50 for microorganisms:
- 429 mg/L
Additional information
No data on the toxicity to microorganisms is available for H2 ED2A. Consistent with the other ecotoxicity endpoints, the available data from H4 EDTA are read-across to fill the datagap. All ecotox, fate and phys-chem data from H4 EDTA, Na2H2 EDTA, Na4 EDTA, HEDTA, H2 ED2A etc have been collated in a document which is compiled to justify the read across. This document is included in IUCLID 6, Chapter 13.
The toxicity of Na2H2 EDTA to microorganisms was tested in an activated sludge respiration inhibition test according to OECD guideline 209 [Van Ginkel & Stroo, 2000. The EC20 value after 30 min was measured to be > 500 mg/L (> 429.5 mg/L H4 EDTA).
For Na4 EDTA a similar low toxicity to activated sludge was measured in an respiration inhibition test according to ISO 8192 [BASF AG, 1988]. In this test a concentration of 1000 mg/L test substance caused no effect on the respiration rate of the bacteria.
Results of nitrification are available by Hu (2003). In a 24 h nitrification inhibition test the EC50 of H4 EDTA to microorganism was determined to be 2.4 mM/L, equivalent to 700 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.