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EC number: 219-102-3 | CAS number: 2359-15-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-09-23 to 1999-10-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- adopted 17 July, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- adopted 29 December, 1992
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- N,N'-methylenebis[methacrylamide]
- EC Number:
- 219-102-3
- EC Name:
- N,N'-methylenebis[methacrylamide]
- Cas Number:
- 2359-15-1
- Molecular formula:
- C9H14N2O2
- IUPAC Name:
- N,N'-methylenebis(2-methylacrylamide)
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from the aeration tank of a municipal biological waste water treatment plant, not adapted, not pre-conditioned. The activated sludge was used immediately after sampling from the treatment plant without adaptation.
- Storage conditions: none
- Preparation of inoculum for exposure: Prior to the test the sludge was washed twice with tap water.
- Concentration of sludge: 30 mg/L
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- O2 consumption was determined compared to the theoretical O2 demand
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: see Table 1
- Test temperature: 22 ±
- pH: 7.4 ± 0.2
- pH adjusted: yes with NaOH or H2SO4
- Suspended solids concentration: 30 mg/L
TEST SYSTEM
- Culturing apparatus: The test vessels were agitated on an orbital shaker (100 r.p.m.) for a maximum test period of 28 days.
- Number of culture flasks/concentration: 2
- Measuring equipment: O2 uptake was measured by gas chromatography. Dissolved organic carbon (DOC) was determined in duplicate with a Shimadzu 5050 TOO-Analyzer using the NPOC-mode. For each determination 3 single injections were performed. The chemical O2 demand (COD), as a measure of the amount of O2 from potassium chromate that reacts with the oxidizable test substance, was determined using the Spectroquant COD Cell Test (E. MERCK, Darmstadt, Germany).
SAMPLING
- Sampling frequency: Samples were taken on day 0, 1, 4, 8, 12, 14, 18, 21, 25, and 28.
CONTROL AND BLANK SYSTEM
- Inoculum blank: lnoculum blank containing inoculum and test mediummeasured in two replicates
- Abiotic sterile control: not conducted
- Toxicity control: not conducted
STATISTICAL METHODS:
The percentage biodegradation was calculated from the BOD determinations and the ThOD or COD using the following equation: D = BOD/ThOD x 100 or D = BOD/COD x 100
D is the degradation at time t in %
ThOD is the theoretical O2 demand of the test substance in mg calculated from the elemental composition of the test substance (Cc,Hh,Clcl,Nn,Nana,Oo,Pp,Ss) according to: ThOD = 16(2c + 0.5(h-cl-3n) + 3s + 2.5p + 0.5na - o) mg/mg / Molecular weight
or, in case where nitrification of the nitrogen is assumed, according to: ThOD = 16(2c + 0.5(h - cl) + 2.5n + 3s + 2.5p + 0.5na - o) mg/mg / Molecular weight
BOD = a -bm/Co x V = mg O2 uptake by test substance - mg O2 uptake by blank / mg test substance in vessel
a is the mean O2 uptake of the two test units at time of determination in mg/L
bm is the mean O2 uptake of the mean of two inoculum blanks at time of determination in mg/L
C0 is the initial nominal concentration of the test substance in mg/L
V is the total volume of the test suspension in L
The mean percentage biodegradation of the test article rounded to the nearest full percent was specifically calculated at the end of the test and at the end of the 10-d window. The ultimate biodegradation was calculated from the DOC determinations as follows:
DT = [1- CT-C(B)T/ Co - C(B)o] x 100
DT is the degradation at the end of test in %
CT is the mean DOC value of the test unit at the end of the test in mg/L
C(B)T is the mean DOC value of the inoculum blank at the end of the test in mg/L
C(B)0 is the mean initial DOC value of the inoculum blank in mg/L
C0 is the mean initial DOC value in the test unit in mg/L
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1.3
- Sampling time:
- 8 d
- Remarks on result:
- other: Two replicates no standard deviation was measured
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1.6
- Sampling time:
- 12 d
- Remarks on result:
- other: Two replicates no standard deviation was measured
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 17.4
- Sampling time:
- 28 d
- Remarks on result:
- other: Two replicates no standard deviation was measured
BOD5 / COD results
- Results with reference substance:
- The degradation in precent of sodium benzoate was 69.8 % after 8 days, 76.8 % after 12 days and 87.5 % after 28 days.
Any other information on results incl. tables
Table 2: O2 uptake of the test units, the inoculum blank and the corresponding degradation data
|
Inoculum + blank |
Test unit No. 1 containing test material |
Test unit No. 2 containing test material |
|
||||
Time (days) |
Total O2 uptake in test sample (mg) |
Total O2 uptake in test sample (mg) |
BOD (mgO2/mg Substance) |
Degradation (%) |
Total O2 uptake in test sample (mg) |
BOD (mgO2/mg Substance) |
Degradation (%) |
Mean degradation of 1 +2 in (%) |
0 |
0.00 |
0.00 |
0.00 |
0.0 |
0.00 |
0.00 |
0.0 |
0.0 |
1 |
-0.11 |
1.23 |
0.03 |
1.4 |
0.46 |
0.01 |
0.6 |
1.0 |
4 |
4.18 |
4.76 |
0.01 |
0.6 |
5.40 |
0.03 |
1.2 |
0.9 |
8 |
4.54 |
5.50 |
0.02 |
1.0 |
6.22 |
0.04 |
1.7 |
1.3 |
12 |
9.49 |
10.73 |
0.03 |
1.3 |
11.38 |
0.05 |
1.9 |
1.6 |
14 |
6.67 |
8.29 |
0.04 |
1.6 |
9.11 |
0.06 |
2.5 |
2.1 |
18 |
7.89 |
8.98 |
0.03 |
1.1 |
10.25 |
0.06 |
2.4 |
1.8 |
21 |
9.53 |
11.03 |
0.04 |
1.5 |
10.51 |
0.02 |
1.0 |
1.3 |
25 |
10.92 |
16.77 |
0.15 |
5.9 |
17.21 |
0.16 |
6.4 |
6.2 |
28 |
10.72 |
25.52 |
0.37 |
15.0 |
30.21 |
0.49 |
19.8 |
17.4 |
Table 3: O2 uptake of the procedure control
|
Inoculum + blank |
Procedure control containing sodium bezoate |
||
Time (days) |
Total O2 uptake in test sample (mg) |
Total O2 uptake in test sample (mg) |
BOD (mgO2/mg Substance) |
Degradation (%) |
0 |
0.00 |
0.00 |
0.00 |
0.0 |
1 |
-0.11 |
2.56 |
0.07 |
4.0 |
4 |
4.18 |
40.10 |
0.90 |
53.9 |
8 |
4.54 |
51.03 |
1.16 |
69.8 |
12 |
9.49 |
60.64 |
1.28 |
76.8 |
14 |
6.67 |
62.09 |
1.39 |
83.2 |
18 |
7.89 |
64.27 |
1.41 |
84.7 |
21 |
9.53 |
65.81 |
1.41 |
84.5 |
25 |
10.92 |
67.79 |
1.42 |
85.4 |
28 |
10.72 |
68.99 |
1.46 |
87.5 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- In this study, the degradation of the test item was 17 % after 28 days. The substance is therefore considered to be not readily biodegradable under the conditions of the test.
- Executive summary:
In the present study conducted according to OECD test guideline 301F (adopted 27 July, 1992) activated sludge from a waste water treatment plant was incubated with N,N’-methylenebis[methacrylamide] for 28 days under aerobic, static conditions. During the test the oxygen consumption and the DOC concentrations were determined. The degradation of N,N’-methylenebis[methacrylamide] was 17 % after 28 days, thus the substance did not pass the 10 days window and was not degraded during the study. N,N’-methylenebis[methacrylamide] is therefore considered to be not readily biodegradable under the conditions of the test.
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