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EC number: 202-358-5 | CAS number: 94-71-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 18 march 2010 to 15 july 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study conducted according to standardised guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-ethoxyphenol
- EC Number:
- 202-358-5
- EC Name:
- 2-ethoxyphenol
- Cas Number:
- 94-71-3
- Molecular formula:
- C8H10O2
- IUPAC Name:
- 2-ethoxyphenol
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
The concentrations of the test item GUETOL were analyzed in the duplicate test media samples from the nominal concentrations of 3.2 to 100 mg/L from both sampling times (0 and 48 hours). The samples of the nominal test concentration of 1.0 mg/L were not analyzed since the concentration was below the 48 hour NOEC determined in this test.
- Sampling method:
For the determination of the actual test item concentrations, duplicate samples were taken from each treatment before the test start and at the end of the test after 48 hours.
For the 48-hour stability samples, the contents of the respective replicates were combined prior to sampling.
From the control, only one of the duplicate samples was analyzed per sampling time.
- Sample storage conditions before analysis:
All samples were stored deep-frozen (at about -20 °C) immediately after sampling. Based on pre-experiments for investigation of the storage stability (non-GLP), the test item was found to be stable in the test water under these storage conditions.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest nominal concentration of 100 mg/L was prepared in a completely filled and tightly closed stirring vessel by dissolving 58.6 mg of the test item in 580 mL of test water stirring intensely for 10 minutes at room temperature. Adequate volumes of this test medium were diluted with test water to prepare the test media with the lower test item concentrations. The test media were prepared just before introduction of the daphnids (i.e., start of the test).
- Eluate: No
- Differential loading: No
- Controls: a control (test water without test item) was tested in parallel; four replicates.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The appearance of the test media was visually recorded at the start of the test and after 24 and 48 hours. No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions throughout the entire test duration.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus, Clone 5
- Source: originally supplied by the University of Sheffield/UK in 1992.
- Age at study initiation): At the start of the test, the test organisms were 6-24 hours old and were not be first brood progeny.
- Method of breeding: The clone (defined by the supplier as clone 5) is successfully bred in Harlan Laboratories under temperature and light conditions identical to those of the tests. The cultivation of the parental daphnids is performed in reconstituted water of the quality identical to the water quality used in the tests (in respect to pH, main ions, and total hardness). During breeding, daphnids are generally fed three times a week with an algal suspension of the green algae Desmodesmus subspicatus CHODAT, Strain No. 86.81 SAG or a mixture of this algal suspension and a commercial fish diet (Tetra Min® Hauptfutter, supplied by TETRA-Werke, 49304 Melle / Germany).
- Feeding during test: no
ACCLIMATION: not applicable
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- The test was performed in a temperature-controlled room with continuous monitoring of the room temperature. The water temperature was maintained at 20-21 °C.
- pH:
- At the beginning and end of the test period, the pH value of the test media was in the range of 7.8 to 7.9
- Dissolved oxygen:
- The test water was aerated prior to the start of the study until oxygen saturation was reached. During the test period, the test water was not aerated. At the beginning and end of the test period, the dissolved oxygen concentrations in the test media and control were at least 8.2 mg/L,
- Nominal and measured concentrations:
- Nominal: 1.0, 3.2, 10, 32, and 100 mg/L.
Measured: At the start and at the end of the test, the analytically determined concentrations of the test item in the test media were between 96 and 103% of the nominal values. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50-mL glass test tubes
- Type (delete if not applicable): closed; The test tubes were made tight with glass stoppers to avoid losses of the volatile substance.
- Material, size, headspace, fill volume: glass/ 50 mL/ 0/about 50 mL of test medium
- Aeration: The test water was aerated prior to the start of the study until oxygen saturation was reached. During the test period, the test water was not aerated.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): NA
- Biomass loading rate: 1 daphnia per mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted test water according to ISO 6341 was used in the study. It consisted of analytical grade salts dissolved in purified water at the following nominal concentrations
CaCl2 × 2H2O: 294 mg/L
MgSO4 × 7H2O: 123 mg/L
NaHCO3: 65 mg/L
KCl: 5.8 mg/L
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4
- Conductivity: no data
- Culture medium different from test medium: no
- Intervals of water quality measurement: At the start and at the end of the test, the pH values, dissolved oxygen concentrations and water temperature were determined at each treatment.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16-hour light to 8-hour dark cycle with a 30-minute transition period.
- Light intensity: approximately between 520 and 680 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study
- Test concentrations: 0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits: 14-20 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits: 14-20 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: not reported
- Observations on body length and weight: NA
- Other biological observations: not reported
- Mortality of control: no daphnids showed immobilization or other signs of disease or stress (e.g., discoloration or unusual behavior such as trapping at the surface water).
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions throughout the entire test duration.
- Effect concentrations exceeding solubility of substance in test medium: NA - Results with reference substance (positive control):
- The result of the latest positive control test in March 2010 (48-hour EC50: 0.43 mg/L,study C86933) proved the sensitivity of the test organisms and the validity of the test design (internal historical range of 48-hour EC50 from 2000 to 2010: 0.43‑1.1 mg/L).
- Reported statistics and error estimates:
- The 24- and 48-hour EC50 and the 95% confidence limits were calculated by Probit Analysis using linear maximum likelyhood regression.
Any other information on results incl. tables
At the start and end of the test, the analytically determined concentrations of the test item in the test media were between 96 and 103% of the nominal values (see analytical results and Table 2 in the corresponding appendix). Thus, the correct dosing of the test item GUETOL was confirmed. Furthermore, it has been demonstrated that the test item was stable during the test period of 48 hours under the conditions of the test, and the reported biological results are based on the nominal concentrations of the test item.
Effect of GUETOL on the Mobility of Daphnia magna in the definitive test
Nominal |
No. of |
Immobilized |
Immobilized |
||
(mg/L) |
|
No. |
% |
No. |
% |
Control |
20 |
0 |
0 |
0 |
0 |
1.0 |
20 |
0 |
0 |
0 |
0 |
3.2 |
20 |
0 |
0 |
0 |
0 |
10 |
20 |
1 |
5 |
1 |
5 |
32 |
20 |
20 |
100 |
20 |
100 |
100 |
20 |
20 |
100 |
20 |
100 |
Effect of GUETOL on the Mobility of Daphnia magna in the range finding test
Nominal test item concentration
|
No. of |
Immobilized |
Immobilized |
||
(mg/L) |
|
No. |
% |
No. |
% |
Control |
10 |
0 |
0 |
0 |
0 |
1.0 |
10 |
0 |
0 |
1 |
0 |
10 |
10 |
10 |
100 |
10 |
10 |
100 |
10 |
10 |
100 |
10 |
100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- In the control, no daphnids showed immobilization or other signs of disease or stress. Furthermore, the dissolved oxygen concentration at the end of the test was ≥3 mg/L in the control and test vessels.
- Conclusions:
- This study is classified as Klimisch 1 and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Based on the results of this study, guetol would be considered as harmful for aquatic invertebrates. - Executive summary:
The acute toxicity of the test item GUETOL to Daphnia magna was determined in a 48‑hour static test (Harlan, 2010), according to the EU C.2, and the OECD 202.
The nominal test item concentrations tested were 1.0, 3.2, 10, 32, and 100 mg/L. Additionally, a control group (test water without test item) was tested in parallel. The test was performed using glass tubes completely filled with test medium that were tightly sealed with glass stoppers to avoid losses of test item (closed system).
At the start and at the end of the test, the analytically determined concentrations of the test item in the test media were between 96 and 103% of the nominal values. Thus, the correct dosing of the test item GUETOL was confirmed. Furthermore, it has been demonstrated that the test item was stable during the test period of 48 hours under the conditions of the test, and the reported biological results are based on the nominal concentrations of the test item.
The biological test result was as follows:
– 48-hour EC50:
16 mg/L (95%CL: 14 and 20 mg/L)
Based on the results of this study, guetol would be considered as harmful for aquatic invertebrates.
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