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EC number: 915-277-1 | CAS number: 32052-51-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
The study on skin irritation revealed, with regard to the irritation index (5.13 of max. 8.0), a “highly irritating” potential of the test substance.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1984-09-25 to 1984-10-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- (1981)
- Deviations:
- no
- GLP compliance:
- no
- Species:
- rabbit
- Strain:
- other: Small white Russian
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS:
- Strain: Small white Russian, Chbb-SPF
- Sex: male and female
- Source: Dr. Karl Thomae GmbH, Biberach (Germany)
- Weight at study initiation: 2.2 - 2.6 kg
- Number of animals: 3 males, 3 females
Housing conditions:
- Room temperature: 20°C (+/- 1°C)
- Humidity: 60% (+/- 5%); light: 12 hours/day
- Air exchange: 15-fold/h
- Access to drinking water: ad libitum
- Diet: K4 diet for rabbits (Ssniff; Soest) - Type of coverage:
- occlusive
- Preparation of test site:
- shaved
- Vehicle:
- unchanged (no vehicle)
- Amount / concentration applied:
- 0.5 ml, undiluted
- Duration of treatment / exposure:
- 4 hour(s)
- Number of animals:
- 6
- Details on study design:
- ADMINISTRATION/EXPOSURE
- Area of exposure: 6 cm2
- Total volume applied: 0.5 ml
- Occlusion: mull patch, polyethylene film, elastic dressing
- Removal of test substance: washing with deionized water
- Post exposure period: 3 weeks
EXAMINATIONS
- Scoring system: OECD; evaluation: VCI notes on safety data sheet and Appendix VI of 79/831/EEC
- Examination time points: 1, 24, 48, 72 hours, 6, 8, 10, 14, and 21 days after patch removal; evaluation based on readings 1-4 - Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: 1, 24, 48, 72 h
- Score:
- 5.13
- Max. score:
- 8
- Reversibility:
- not reversible
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 24, 48, 72 h
- Score:
- 2.67
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 24, 48, 72 h
- Score:
- 2.44
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritant / corrosive response data:
- AVERAGE SCORE
- Erythema: 2.67
- Edema: 2.44
- irritation index: 5.13/8
REVERSIBILITY: Not reversible. Necrosis within 4 hours, not within 3 minutes. Eschars, incrustation, or scars by end of study in all animals. - Other effects:
- no other effects
- Conclusions:
- Under the conditions of this study, the test item 2,2,4 -/2,4,4 -trimethylhexane-1,6 -diisocyanate showed severe damage on the skin
of male and female rabbits. All rabbits showed eschars, incrustation, or scars by end of study. - Executive summary:
A study was performed to examine skin irritating effects of 2,2,4-/2,4,4-trimethylhexane-1,6-diisocyanate according to OECD TG 404 in rabbits. Undiluted test substance was applied in the study. In the study three rabbits per sex were exposed occlusively for four hours. Necrosis within 4 hours, not within 3 minutes was observed . All rabbits showed eschars, incrustation, or scars by end of study. The result was, with regard to the irritation index (5.13 of max. 8.0), described as “highly irritating”.
Reference
no further results
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1984-09-25 to 1984-10-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- (1981)
- Deviations:
- no
- GLP compliance:
- no
- Species:
- rabbit
- Strain:
- other: Small white Russian
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS:
- Strain: Small white Russian, Chbb-SPF
- Sex: male/female - Source: Dr. Karl Thomae GmbH, Biberach (Germany)
- Weight at study initiation: 2.3 - 2.5 kg
- Number of animals: 3 males, 3 females
- Controls: untreated eye
Housing conditions:
- Room temperature: 20°C (+/- 1°C)
- Humidity: 60% (+/- 5%); light: 12 hours/day
- Air exchange: 15-fold/h
- Access to drinking water: ad libitum
- Diet: K4 diet for rabbits (Ssniff; Soest) - Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated eye
- Amount / concentration applied:
- undiluted
Amount applied: 0.1 ml - Duration of treatment / exposure:
- not rinsed
- Observation period (in vivo):
- 1, 24, 48, 72 hours, 6, 8, 10, 13, 15, 17, and 21 days after treatment
- Number of animals or in vitro replicates:
- 3 males, 3 females
- Details on study design:
- EXAMINATIONS
- Ophtalmoscopic examination: 1, 24, 48, 72 hours, 6, 8, 10, 13, 15, 17, and 21 days after treatment
- Scoring system: Draize (1959); evaluation: VCI notes on safety data sheet and Appendix VI of 79/831/EEC
- Tool used to assess score: sodium fluorescein / ophthalmic lamp / visual inspections - Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.22
- Max. score:
- 4
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0.61
- Max. score:
- 2
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 2.61
- Max. score:
- 3
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.39
- Max. score:
- 4
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 21 d
- Score:
- 23.62
- Max. score:
- 110
- Irritant / corrosive response data:
- AVERAGE SCORE
- Cornea: 1.22
- Iris: 0.61
- Conjunctivae (Redness): 2.61
- Conjunctivae (Chemosis): 1.39
- Overall irritation score: 23.62/110
REVERSIBILITY: Not reversible within 3 weeks. Eyelids were incrusted in 5 animals on days 15 and 17 and in 1 animal on day 21. - Other effects:
- OTHER EFFECTS: Brightness reduced in eyes of 3 animals, redness in iris of all animals (24 until maximum 72 hours), sticking and dryness in the
eyes of the females (days 2-10), eyelids inelastic in all animals from day 3 on and incrusted later, loss of hair in all animals by end of study - Conclusions:
- Under the conditions of this study, the test item 2,2,4 -/2,4,4 -trimethylhexane-1,6 -diisocyanate showed severe damage on the eyes and mucosa
of male and female rabbits.y. The effects are not reversible within 3 weeks. - Executive summary:
The acute eye irritation properties of 2,2,4 -/2,4,4 -trimethylhexane-1,6 -diisocyanate were estimated in a Draize test according to OECD TG 405. An amount of 0.1 ml of the undiluted test substance was instilled into the left eye of three male and three female albino rabbits, and the untreated right eye served as control. The eye remained unrinsed and there was no removal of test substance. The undiluted substance produced reduced brightness in eyes of 3 animals, redness in iris of all animals (24 until maximum 72 hours), sticking and dryness in the eyes of the females (days 2 -10), eyelids inelastic in all animals from day 3 on and incrusted later, loss of hair in all animals by end of study. The effects are not reversible within 3 weeks.
Reference
no further results
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Justification for selection of skin irritation / corrosion endpoint:
key study
Justification for selection of eye irritation endpoint:
key study
Effects on skin irritation/corrosion: highly irritating
Effects on eye irritation: slightly irritating
Justification for classification or non-classification
According to the criteria of EC Directive 67/548/EEC and EC Regulation 1272/2008 and subsequent regulations on classification, labelling and packaging of substances and mixtures and based on the results of the studies for 2,2,4(or 2,4,4)-trimethylhexane-1,6-diisocyanate, the test substance is classified for skin corrosion/irritation as category 1C, H314.
The classification "corrosive to skin" also implies corresponding effects on eyes. Therefore, no classification according this hazard class is necessary based on CLP regulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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