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EC number: 219-330-3 | CAS number: 2416-94-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented study report which meets basic scientific principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,3,6-trimethylphenol
- EC Number:
- 219-330-3
- EC Name:
- 2,3,6-trimethylphenol
- Cas Number:
- 2416-94-6
- Molecular formula:
- C9H12O
- IUPAC Name:
- 2,3,6-trimethylphenol
- Details on test material:
- - Name of test material (as cited in study report): 2,3,6-Trimethylphenol
- Analytical purity: 99.4%
- Impurities (identity and concentrations): no data
- Storage condition of test material: room temperature
No further data.
Constituent 1
Method
- Target gene:
- For Salmonella typhimurium strains, the amino acid histidine locus is the target gene.
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S-9, induced with Aroclor 1254
- Test concentrations with justification for top dose:
- 20 - 5000 µg/plate; see below
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: no data
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene, N-methyl-N'-nitro-N-nitroso-guanidine, 4-nitro-o-phenylendiamine, 9-aminoacridine; depending on strain and activation condition
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation) and preincubation;
- Evaluation criteria:
- In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results - Statistics:
- no data
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >= 2500 µg/plate (SPT); >= 1500 µg/plate (PIT)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
No increase in the number of revertants was observed with any tester strain under any test condition.
Bacteriotoxicity (indicated by reduced bacterial growth) was observed in the SPT at doses >= 2500 µg/plate and in the PIT at 1500 µg/plate.
The test substance was completely soluble in the vehicle chosen; no precipitation was noted at any concentration tested.
Applicant's summary and conclusion
- Executive summary:
REPORT SUMMARY
The substance 2,3,6-Trimethylphenol was tested for mutagenicity in the Ames test.
Strains: TA 1535, TA 100, TA 1537, TA 98
Dose range: 20 µg - 5000 µg/plate (SPT; TA 100, TA 98)
4 µg - 2500 µg/plate (SPT; TA 1535, TA 1537)
4 µg - 1500 µg/plate (PIT; all tester strains )
Test conditions: Standard plate test ( SPT) and preincubation test ( PIT) both with and without metabolic activation (rat liver S-9 mix) .
Solubility: Complete solubility of the test substance in DMSO.
Toxicity: A bacteriotoxic effect was observed in the standard plate test at doses => 2500 µg/plate and in the preincubation test at 1500 µg/plate.
Mutagenicity: An increase in the number of his+ revertants was not observed both in the standard plate test and in the preincubation test either without S-9 mix or after the addition of a metabolizing system.
Assessment:
According to the results of the present study, the test substance 2,3,6-Trimethylphenol is not mutagenic in the Ames test under the experimental conditions chosen here.
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