2,3,6-trimethylphenol

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

no data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented stud report which meets basic scientific principles; pre-GLP study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

2 animals, treatment time: 1, 5, 15 min, 20 hours; onservation after 24 hours and 8 days

Principles of method if other than guideline:

BASF-Test

GLP compliance:

no

Remarks:

pre-GLP study

Test material

Test material information

Constituent 1

Reference substance name:

2,3,6-trimethylphenol

EC Number:

219-330-3

EC Name:

2,3,6-trimethylphenol

Cas Number:

2416-94-6

Molecular formula:

C9H12O

IUPAC Name:

2,3,6-trimethylphenol

Details on test material:

- Name of test material (as cited in study report): 2,3,6-Trimethyl-Phenol (TMP)
Analytical purity: 99%

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

light/dark rhythm: 12/12 hours

Test system

Type of coverage:

semiocclusive

Preparation of test site:

not specified

Vehicle:

water

Controls:

other: untreated sites of the same animals

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): no data
- Concentration (if solution): 50% aqueous suspension
No further data

Duration of treatment / exposure:

1 minute, 5 minutes, 15 minutes, 20 hours (dorsal skin)
20 hours (inner auricle)

Observation period:

8 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: back and ear
- % coverage: no data
- Type of wrap if used: no data

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no data

SCORING SYSTEM:
BASF scores, convertible into Draize scores, readings at 24 hours and 8 days after beginning of application

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 0-8 days

Score:

>= 1 - <= 3

Max. score:

3

Reversibility:

not reversible

Remarks on result:

other: necrosis

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 0-8 days

Score:

0

Max. score:

0

Irritant / corrosive response data:

see table

Any other information on results incl. tables

Draize scores (erythema and edema) and findings:

Site	Exposure  time	24-hour reading		8-day reading
		Animal no. 1	Animal no. 2	Animal no. 1	Animal no. 2
Back	1 min	Erythema: 2	No finding	No finding	No finding
	5 min	Erythema: 2	No finding	No finding	No finding
	15 min	Erythema: 3	Erythema: 1	Slight desquamation	No finding
	20 h	Centre: whitish-anemic Surrounding area: Erythema: 2	Erythema: 2 Necrotic focus (area ca. 1 cm²)	Superficial necrosis	Erythema: 2 Superficial necrosis
Ear	20 h	Erythema: 2 Spotted anemic		Erythema: 2 Superficial necrosis	Encrustation

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Executive summary:

A 50% aqueous preparation applied for up to 20 hours under semiocclusive conditions was irritating to the skin of rabbits.