Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- , see "prinicples of method if other than guideline"
- Principles of method if other than guideline:
- ln OECD Guideline 201 and EU Method C.3, different compositions of stock solution ll are
stated. The study was conducted in accordance with OECD Guideline 201, therefore, the
composition of stock solution ll must be stated as deviation from EU Method C.3. - GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.8 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: limit of water solubility
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 3.8 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: limit of water solubility
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 3.8 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: limit of water solubility
Any other information on results incl. tables
Two experiments were performed.
In the first experiment, the measured values were lower than expected and in the chromatograms,
a contamination with another test item was observed. Therefore, the experiment
was repeated. In the second experiment, a higher concentrated stock solution was used to
prepare a test solution in the range of water solubility. The results of the first experiment
aren't reported.
The main study was performed as a limit test using one concentration in the range of water
solubility. The water solubility was determined as approximately 8 µg/L. At the start and at
the end of the test, the content of the test item in the test solutions was determined using
GC. ln both experiments the measured values at the start of the test were in the same
range (about half of the water solubility). That means the solubility of the test item in algae
medium enriched with nutrient medium and algae was lower than the solubility in water. As
the test item wasn't stable in under test condilions, the measured concentrations test item
after72 hours were lower than LOQ.
The treatment was used to incubate the unicellular freshwater green algae Desmodesmus
subspicatus for a period of 72 hours. The cell concentration of each replicate was determined
by measuring the absorption of the cuvettes at 440 nm every 24 hours with a spectral
photometer. With these measured values, the number of cells was calculated (linear
correlation between cell concentration and absorption given). Then the growth rate, the
area under the growth curve (AUC) and the Yield were determined.
The determination of the results was based on the measured start concentration.
The EC50s of potassium dichromate was tested in a current reference test. The values
were within the normal range of the laboratory.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Allvalidity criteria were met.
The EC50s of potassium dichromate were determined in a current reference test. For the
estimation of the EC50s of the positive control, the fits showed sufficient statistical correspondence
of the data with the dose-response-equation. The values were within the normal
range of the laboratory as well as in correspondence with the values from the international
ring testwhich are stated in EU Method C.3.
The study was performed as a limit test in the range of water solubility. The water solubility
was determined as approximately 8 µg/L. To produce a test solution in the range of water
solubility, a stock solution containing 100 mg/L test item in methanol was used for the
preparation of the test solution (0.1 ml/L) in the first experiment. The measured concentration
at the beginning was lower than expected (about half of water solubility). Therefore the
experiment was repeated using a stock solution containing 200 mg/L.
The measured concentration in the second experiment was in the same range. The lower
measured values might be caused by lower solubility of test item in algae medium enriched
with nutrient medium and alga.
In the both experiments, no inhibition could be observed.
The result of the test can be considered valid.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)
Vaikka kemikaalivirasto tarjoaa paljon verkkoaineistoa kielelläsi, tämä sivu on osittain englanniksi. Lisätietoa viraston monikielisyydestä.
Tervetuloa kemikaaliviraston verkkosivustolle. Internet Explorer 7 (ja aiemmat versiot) ei täysin tue tätä sivustoa. Päivitä Internet Explorerin uudempi versio.
Tällä verkkosivustolla käytetään evästeitä parhaan mahdollisen käyttäjäkokemuksen varmistamiseksi.
Lue lisää evästeiden käytöstä.